Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0103 and Dsui_0104 are separated by 107 nucleotides Dsui_0104 and Dsui_0105 are separated by 64 nucleotides Dsui_0105 and Dsui_0106 are separated by 252 nucleotides
Dsui_0103: Dsui_0103 - transcriptional regulator, at 101,774 to 102,364
_0103
Dsui_0104: Dsui_0104 - CBS-domain-containing membrane protein, at 102,472 to 102,966
_0104
Dsui_0105: Dsui_0105 - NAD-dependent aldehyde dehydrogenase, at 103,031 to 104,461
_0105
Dsui_0106: Dsui_0106 - hypothetical protein, at 104,714 to 105,490
_0106
Position (kb)
103
104
105 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 102.071 kb on - strand, within Dsui_0103 at 102.076 kb on - strand, within Dsui_0103 at 102.157 kb on + strand at 102.168 kb on + strand at 102.176 kb on - strand, within Dsui_0103 at 102.186 kb on - strand, within Dsui_0103 at 102.223 kb on + strand at 102.352 kb on - strand at 102.368 kb on - strand at 102.495 kb on + strand at 102.511 kb on + strand at 102.511 kb on + strand at 102.511 kb on + strand at 102.519 kb on - strand at 102.519 kb on - strand at 102.542 kb on + strand at 102.585 kb on - strand at 102.696 kb on + strand, within Dsui_0104 at 102.728 kb on + strand, within Dsui_0104 at 102.748 kb on + strand at 102.842 kb on - strand, within Dsui_0104 at 102.965 kb on - strand at 102.967 kb on - strand at 102.975 kb on - strand at 103.104 kb on - strand at 103.125 kb on - strand at 103.156 kb on - strand at 103.158 kb on + strand at 103.246 kb on - strand, within Dsui_0105 at 103.324 kb on - strand at 103.544 kb on - strand, within Dsui_0105 at 103.548 kb on - strand, within Dsui_0105 at 103.586 kb on - strand at 103.592 kb on - strand at 103.613 kb on + strand at 103.726 kb on + strand at 103.726 kb on + strand, within Dsui_0105 at 103.734 kb on - strand at 103.734 kb on - strand, within Dsui_0105 at 103.734 kb on - strand, within Dsui_0105 at 103.785 kb on + strand, within Dsui_0105 at 103.829 kb on + strand at 103.830 kb on + strand at 103.926 kb on - strand, within Dsui_0105 at 104.029 kb on - strand at 104.042 kb on - strand, within Dsui_0105 at 104.072 kb on + strand, within Dsui_0105 at 104.073 kb on - strand, within Dsui_0105 at 104.149 kb on - strand, within Dsui_0105 at 104.211 kb on + strand at 104.227 kb on + strand at 104.463 kb on + strand at 104.494 kb on + strand at 104.502 kb on - strand at 104.513 kb on + strand at 104.551 kb on - strand at 104.553 kb on + strand at 104.696 kb on + strand at 104.715 kb on - strand at 104.715 kb on - strand at 104.744 kb on - strand at 104.955 kb on - strand, within Dsui_0106 at 104.965 kb on + strand, within Dsui_0106 at 104.984 kb on + strand at 104.997 kb on - strand, within Dsui_0106 at 105.068 kb on + strand, within Dsui_0106 at 105.076 kb on + strand at 105.113 kb on - strand, within Dsui_0106 at 105.202 kb on + strand, within Dsui_0106 at 105.210 kb on - strand at 105.210 kb on - strand, within Dsui_0106 at 105.275 kb on - strand, within Dsui_0106 at 105.458 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 102,071 - Dsui_0103 0.50 +0.1 102,076 - Dsui_0103 0.51 +0.3 102,157 + -1.2 102,168 + -1.2 102,176 - Dsui_0103 0.68 +0.7 102,186 - Dsui_0103 0.70 +0.3 102,223 + -0.2 102,352 - +0.2 102,368 - +0.8 102,495 + -0.2 102,511 + +0.8 102,511 + +0.8 102,511 + -0.2 102,519 - -0.2 102,519 - -0.8 102,542 + +0.8 102,585 - -0.2 102,696 + Dsui_0104 0.45 -1.8 102,728 + Dsui_0104 0.52 -0.2 102,748 + -0.2 102,842 - Dsui_0104 0.75 +0.8 102,965 - -0.8 102,967 - -0.8 102,975 - -1.5 103,104 - +0.8 103,125 - +0.8 103,156 - +2.5 103,158 + +0.8 103,246 - Dsui_0105 0.15 +0.8 103,324 - -0.8 103,544 - Dsui_0105 0.36 -1.2 103,548 - Dsui_0105 0.36 -0.2 103,586 - +0.8 103,592 - +0.8 103,613 + -0.8 103,726 + +0.8 103,726 + Dsui_0105 0.49 -0.8 103,734 - -0.2 103,734 - Dsui_0105 0.49 -2.7 103,734 - Dsui_0105 0.49 -1.5 103,785 + Dsui_0105 0.53 -2.4 103,829 + -0.2 103,830 + -0.2 103,926 - Dsui_0105 0.63 -2.1 104,029 - +0.8 104,042 - Dsui_0105 0.71 +0.8 104,072 + Dsui_0105 0.73 -0.8 104,073 - Dsui_0105 0.73 -0.8 104,149 - Dsui_0105 0.78 -1.7 104,211 + +0.8 104,227 + -0.2 104,463 + -0.2 104,494 + +0.8 104,502 - -0.8 104,513 + +0.8 104,551 - +0.8 104,553 + +0.8 104,696 + -0.8 104,715 - +0.0 104,715 - -0.8 104,744 - +0.8 104,955 - Dsui_0106 0.31 -0.2 104,965 + Dsui_0106 0.32 -1.5 104,984 + -1.2 104,997 - Dsui_0106 0.36 -1.8 105,068 + Dsui_0106 0.46 -0.2 105,076 + +0.8 105,113 - Dsui_0106 0.51 -0.8 105,202 + Dsui_0106 0.63 -2.4 105,210 - +3.6 105,210 - Dsui_0106 0.64 -1.2 105,275 - Dsui_0106 0.72 -0.8 105,458 - -2.1
Or see this region's nucleotide sequence