Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0087 and Dsui_0088 are separated by 178 nucleotides Dsui_0088 and Dsui_0089 are separated by 55 nucleotides
Dsui_0087: Dsui_0087 - mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase, at 85,418 to 86,854
_0087
Dsui_0088: Dsui_0088 - tRNA-Ala, at 87,033 to 87,108
_0088
Dsui_0089: Dsui_0089 - methyl-accepting chemotaxis protein, at 87,164 to 88,798
_0089
Position (kb)
87
88 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 86.184 kb on + strand, within Dsui_0087 at 86.218 kb on + strand, within Dsui_0087 at 86.284 kb on + strand, within Dsui_0087 at 86.292 kb on - strand, within Dsui_0087 at 86.394 kb on - strand, within Dsui_0087 at 86.433 kb on - strand, within Dsui_0087 at 86.455 kb on - strand, within Dsui_0087 at 86.544 kb on - strand, within Dsui_0087 at 86.554 kb on - strand, within Dsui_0087 at 86.564 kb on - strand, within Dsui_0087 at 86.584 kb on + strand, within Dsui_0087 at 86.698 kb on + strand at 86.698 kb on + strand, within Dsui_0087 at 86.801 kb on - strand at 86.825 kb on - strand at 86.833 kb on + strand at 86.879 kb on + strand at 86.919 kb on + strand at 86.929 kb on + strand at 86.929 kb on + strand at 86.937 kb on - strand at 86.973 kb on + strand at 87.016 kb on + strand at 87.017 kb on - strand at 87.021 kb on + strand at 87.040 kb on - strand at 87.040 kb on - strand at 87.056 kb on + strand, within Dsui_0088 at 87.064 kb on - strand, within Dsui_0088 at 87.087 kb on + strand, within Dsui_0088 at 87.094 kb on - strand, within Dsui_0088 at 87.136 kb on + strand at 87.136 kb on + strand at 87.136 kb on + strand at 87.136 kb on + strand at 87.136 kb on + strand at 87.136 kb on + strand at 87.144 kb on - strand at 87.144 kb on - strand at 87.180 kb on - strand at 87.237 kb on + strand at 87.250 kb on - strand at 87.278 kb on - strand at 87.284 kb on - strand at 87.323 kb on - strand at 87.330 kb on + strand, within Dsui_0089 at 87.340 kb on + strand, within Dsui_0089 at 87.343 kb on - strand, within Dsui_0089 at 87.402 kb on + strand, within Dsui_0089 at 87.490 kb on + strand, within Dsui_0089 at 87.549 kb on - strand, within Dsui_0089 at 87.564 kb on - strand, within Dsui_0089 at 87.587 kb on - strand, within Dsui_0089 at 87.768 kb on + strand, within Dsui_0089 at 87.783 kb on + strand, within Dsui_0089 at 87.818 kb on + strand, within Dsui_0089 at 87.882 kb on - strand, within Dsui_0089 at 87.882 kb on - strand, within Dsui_0089 at 87.910 kb on - strand, within Dsui_0089 at 87.910 kb on - strand, within Dsui_0089 at 87.976 kb on + strand, within Dsui_0089 at 87.990 kb on + strand, within Dsui_0089 at 88.064 kb on + strand, within Dsui_0089 at 88.067 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 86,184 + Dsui_0087 0.53 +0.8 86,218 + Dsui_0087 0.56 -0.2 86,284 + Dsui_0087 0.60 -0.2 86,292 - Dsui_0087 0.61 -3.4 86,394 - Dsui_0087 0.68 -2.8 86,433 - Dsui_0087 0.71 -3.2 86,455 - Dsui_0087 0.72 -2.4 86,544 - Dsui_0087 0.78 -3.6 86,554 - Dsui_0087 0.79 -2.8 86,564 - Dsui_0087 0.80 -2.7 86,584 + Dsui_0087 0.81 -0.2 86,698 + +0.8 86,698 + Dsui_0087 0.89 +0.8 86,801 - -3.0 86,825 - -2.9 86,833 + +0.8 86,879 + -1.8 86,919 + +0.8 86,929 + +0.8 86,929 + -0.8 86,937 - -0.3 86,973 + +0.8 87,016 + -1.2 87,017 - -1.2 87,021 + +0.8 87,040 - -0.2 87,040 - +0.0 87,056 + Dsui_0088 0.30 +0.8 87,064 - Dsui_0088 0.41 -0.1 87,087 + Dsui_0088 0.71 -0.2 87,094 - Dsui_0088 0.80 -0.2 87,136 + -0.2 87,136 + -1.2 87,136 + +0.8 87,136 + -0.2 87,136 + -1.2 87,136 + +0.8 87,144 - +1.3 87,144 - +0.0 87,180 - +0.0 87,237 + -0.8 87,250 - +0.6 87,278 - -0.0 87,284 - +0.1 87,323 - -0.8 87,330 + Dsui_0089 0.10 +0.8 87,340 + Dsui_0089 0.11 -0.8 87,343 - Dsui_0089 0.11 -0.6 87,402 + Dsui_0089 0.15 +0.8 87,490 + Dsui_0089 0.20 -1.5 87,549 - Dsui_0089 0.24 +0.1 87,564 - Dsui_0089 0.24 +0.8 87,587 - Dsui_0089 0.26 +0.2 87,768 + Dsui_0089 0.37 -0.2 87,783 + Dsui_0089 0.38 -0.2 87,818 + Dsui_0089 0.40 -0.2 87,882 - Dsui_0089 0.44 +0.2 87,882 - Dsui_0089 0.44 +0.7 87,910 - Dsui_0089 0.46 +0.1 87,910 - Dsui_0089 0.46 -0.2 87,976 + Dsui_0089 0.50 +0.8 87,990 + Dsui_0089 0.51 +0.8 88,064 + Dsui_0089 0.55 +0.8 88,067 + +0.8
Or see this region's nucleotide sequence