Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0047 and Dsui_0048 are separated by 165 nucleotides Dsui_0048 and Dsui_0049 are separated by 43 nucleotides
Dsui_0047: Dsui_0047 - putative integral membrane protein, at 43,748 to 44,518
_0047
Dsui_0048: Dsui_0048 - phosphoribosylaminoimidazole-succinocarboxamide synthase, at 44,684 to 45,622
_0048
Dsui_0049: Dsui_0049 - uncharacterized membrane-anchored protein, at 45,666 to 46,976
_0049
Position (kb) 44
45
46 Strain fitness (log2 ratio) -2
-1
0
1 at 43.694 kb on + strand at 43.815 kb on - strand at 43.851 kb on - strand, within Dsui_0047 at 43.851 kb on - strand, within Dsui_0047 at 43.851 kb on - strand, within Dsui_0047 at 43.874 kb on - strand, within Dsui_0047 at 44.022 kb on - strand, within Dsui_0047 at 44.184 kb on - strand, within Dsui_0047 at 44.535 kb on + strand at 44.547 kb on + strand at 44.563 kb on + strand at 44.595 kb on + strand at 44.632 kb on - strand at 44.701 kb on - strand at 45.632 kb on - strand at 45.666 kb on + strand at 45.681 kb on - strand at 45.681 kb on - strand at 45.802 kb on + strand, within Dsui_0049 at 45.802 kb on + strand at 45.802 kb on + strand at 45.802 kb on + strand at 45.810 kb on - strand, within Dsui_0049 at 45.810 kb on - strand, within Dsui_0049 at 45.812 kb on + strand, within Dsui_0049 at 45.822 kb on - strand, within Dsui_0049 at 45.822 kb on - strand, within Dsui_0049 at 45.863 kb on + strand, within Dsui_0049 at 45.866 kb on + strand, within Dsui_0049 at 45.907 kb on - strand, within Dsui_0049 at 46.044 kb on + strand, within Dsui_0049 at 46.052 kb on - strand, within Dsui_0049 at 46.306 kb on + strand, within Dsui_0049 at 46.323 kb on + strand, within Dsui_0049 at 46.389 kb on + strand, within Dsui_0049 at 46.416 kb on - strand, within Dsui_0049 at 46.607 kb on + strand, within Dsui_0049
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 43,694 + -2.0 43,815 - +0.4 43,851 - Dsui_0047 0.13 -0.2 43,851 - Dsui_0047 0.13 -0.1 43,851 - Dsui_0047 0.13 -0.1 43,874 - Dsui_0047 0.16 -0.5 44,022 - Dsui_0047 0.36 -0.5 44,184 - Dsui_0047 0.57 -0.0 44,535 + +0.8 44,547 + +0.8 44,563 + +0.8 44,595 + +0.8 44,632 - -0.0 44,701 - +0.9 45,632 - -0.2 45,666 + +0.8 45,681 - -0.1 45,681 - -0.0 45,802 + Dsui_0049 0.10 -0.2 45,802 + +0.8 45,802 + +0.8 45,802 + -0.2 45,810 - Dsui_0049 0.11 +0.2 45,810 - Dsui_0049 0.11 -0.6 45,812 + Dsui_0049 0.11 +0.8 45,822 - Dsui_0049 0.12 +0.7 45,822 - Dsui_0049 0.12 -0.1 45,863 + Dsui_0049 0.15 -1.5 45,866 + Dsui_0049 0.15 -0.2 45,907 - Dsui_0049 0.18 +0.7 46,044 + Dsui_0049 0.29 -0.2 46,052 - Dsui_0049 0.29 +0.2 46,306 + Dsui_0049 0.49 -0.8 46,323 + Dsui_0049 0.50 -1.5 46,389 + Dsui_0049 0.55 -1.8 46,416 - Dsui_0049 0.57 -0.1 46,607 + Dsui_0049 0.72 -1.2
Or see this region's nucleotide sequence