Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0044 and Dsui_0045 are separated by 6 nucleotides Dsui_0045 and Dsui_0046 are separated by 34 nucleotides Dsui_0046 and Dsui_0047 are separated by 111 nucleotides
Dsui_0044: Dsui_0044 - exodeoxyribonuclease III, at 40,231 to 41,004
_0044
Dsui_0045: Dsui_0045 - glutaredoxin-like protein, at 41,011 to 41,520
_0045
Dsui_0046: Dsui_0046 - Zn-dependent oligopeptidase, at 41,555 to 43,636
_0046
Dsui_0047: Dsui_0047 - putative integral membrane protein, at 43,748 to 44,518
_0047
Position (kb) 41
42
43
44 Strain fitness (log2 ratio) -2
-1
0
1
2 at 40.674 kb on + strand, within Dsui_0044 at 40.783 kb on + strand, within Dsui_0044 at 40.873 kb on - strand, within Dsui_0044 at 41.000 kb on - strand at 41.027 kb on + strand at 41.094 kb on - strand, within Dsui_0045 at 41.215 kb on + strand, within Dsui_0045 at 41.274 kb on + strand at 41.279 kb on + strand at 41.324 kb on + strand, within Dsui_0045 at 41.332 kb on - strand, within Dsui_0045 at 41.403 kb on - strand, within Dsui_0045 at 41.459 kb on + strand, within Dsui_0045 at 41.467 kb on - strand, within Dsui_0045 at 41.485 kb on - strand at 41.506 kb on - strand at 41.658 kb on + strand at 41.793 kb on + strand, within Dsui_0046 at 41.825 kb on + strand, within Dsui_0046 at 41.899 kb on - strand, within Dsui_0046 at 41.947 kb on - strand, within Dsui_0046 at 42.048 kb on + strand at 42.095 kb on - strand, within Dsui_0046 at 42.159 kb on + strand, within Dsui_0046 at 42.173 kb on - strand, within Dsui_0046 at 42.245 kb on - strand, within Dsui_0046 at 42.246 kb on - strand, within Dsui_0046 at 42.383 kb on - strand, within Dsui_0046 at 42.506 kb on + strand, within Dsui_0046 at 42.513 kb on + strand, within Dsui_0046 at 42.646 kb on - strand, within Dsui_0046 at 42.679 kb on - strand, within Dsui_0046 at 42.750 kb on + strand at 42.750 kb on + strand, within Dsui_0046 at 42.760 kb on + strand at 42.813 kb on - strand, within Dsui_0046 at 42.821 kb on + strand, within Dsui_0046 at 42.834 kb on - strand, within Dsui_0046 at 42.836 kb on + strand, within Dsui_0046 at 42.896 kb on + strand at 42.981 kb on + strand, within Dsui_0046 at 43.166 kb on - strand, within Dsui_0046 at 43.272 kb on - strand, within Dsui_0046 at 43.292 kb on - strand, within Dsui_0046 at 43.292 kb on - strand, within Dsui_0046 at 43.292 kb on - strand, within Dsui_0046 at 43.311 kb on - strand, within Dsui_0046 at 43.422 kb on + strand at 43.572 kb on - strand at 43.596 kb on - strand at 43.601 kb on - strand at 43.642 kb on + strand at 43.642 kb on + strand at 43.655 kb on - strand at 43.694 kb on + strand at 43.815 kb on - strand at 43.851 kb on - strand, within Dsui_0047 at 43.851 kb on - strand, within Dsui_0047 at 43.851 kb on - strand, within Dsui_0047 at 43.874 kb on - strand, within Dsui_0047 at 44.022 kb on - strand, within Dsui_0047 at 44.184 kb on - strand, within Dsui_0047 at 44.535 kb on + strand at 44.547 kb on + strand at 44.563 kb on + strand at 44.595 kb on + strand at 44.632 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 40,674 + Dsui_0044 0.57 -0.2 40,783 + Dsui_0044 0.71 -2.4 40,873 - Dsui_0044 0.83 -0.2 41,000 - -0.2 41,027 + +0.8 41,094 - Dsui_0045 0.16 -0.1 41,215 + Dsui_0045 0.40 -0.8 41,274 + +0.8 41,279 + +0.8 41,324 + Dsui_0045 0.61 -1.2 41,332 - Dsui_0045 0.63 -0.2 41,403 - Dsui_0045 0.77 +0.2 41,459 + Dsui_0045 0.88 -1.5 41,467 - Dsui_0045 0.89 +0.1 41,485 - -0.0 41,506 - -0.3 41,658 + -0.8 41,793 + Dsui_0046 0.11 -0.2 41,825 + Dsui_0046 0.13 -0.8 41,899 - Dsui_0046 0.17 +0.9 41,947 - Dsui_0046 0.19 +0.8 42,048 + +0.8 42,095 - Dsui_0046 0.26 +1.1 42,159 + Dsui_0046 0.29 +0.8 42,173 - Dsui_0046 0.30 +0.5 42,245 - Dsui_0046 0.33 +1.4 42,246 - Dsui_0046 0.33 +1.0 42,383 - Dsui_0046 0.40 +1.3 42,506 + Dsui_0046 0.46 -2.4 42,513 + Dsui_0046 0.46 -0.8 42,646 - Dsui_0046 0.52 +1.0 42,679 - Dsui_0046 0.54 +2.0 42,750 + +0.8 42,750 + Dsui_0046 0.57 -0.2 42,760 + +0.8 42,813 - Dsui_0046 0.60 +1.1 42,821 + Dsui_0046 0.61 -0.8 42,834 - Dsui_0046 0.61 +1.9 42,836 + Dsui_0046 0.62 +0.8 42,896 + +0.8 42,981 + Dsui_0046 0.68 +0.8 43,166 - Dsui_0046 0.77 +1.3 43,272 - Dsui_0046 0.82 +0.8 43,292 - Dsui_0046 0.83 +1.2 43,292 - Dsui_0046 0.83 +1.3 43,292 - Dsui_0046 0.83 +1.0 43,311 - Dsui_0046 0.84 +1.0 43,422 + +0.8 43,572 - +0.9 43,596 - +1.5 43,601 - +1.8 43,642 + -1.8 43,642 + -0.8 43,655 - +0.5 43,694 + -2.0 43,815 - +0.4 43,851 - Dsui_0047 0.13 -0.2 43,851 - Dsui_0047 0.13 -0.1 43,851 - Dsui_0047 0.13 -0.1 43,874 - Dsui_0047 0.16 -0.5 44,022 - Dsui_0047 0.36 -0.5 44,184 - Dsui_0047 0.57 -0.0 44,535 + +0.8 44,547 + +0.8 44,563 + +0.8 44,595 + +0.8 44,632 - -0.0
Or see this region's nucleotide sequence