Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0019 and Dsui_0020 are separated by 110 nucleotides Dsui_0020 and Dsui_0021 overlap by 8 nucleotides Dsui_0021 and Dsui_0022 are separated by 109 nucleotides
Dsui_0019: Dsui_0019 - malate synthase A, at 16,558 to 18,156
_0019
Dsui_0020: Dsui_0020 - HAD-superfamily subfamily IIA hydrolase, TIGR01458, at 18,267 to 19,067
_0020
Dsui_0021: Dsui_0021 - putative metalloendopeptidase, at 19,060 to 19,293
_0021
Dsui_0022: Dsui_0022 - hypothetical protein, at 19,403 to 20,341
_0022
Position (kb) 19
20 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 18.098 kb on + strand at 18.120 kb on + strand at 18.145 kb on + strand at 18.146 kb on - strand at 18.151 kb on + strand at 18.216 kb on + strand at 18.224 kb on - strand at 18.231 kb on - strand at 18.231 kb on - strand at 18.233 kb on + strand at 18.233 kb on + strand at 18.278 kb on + strand at 18.284 kb on + strand at 18.290 kb on - strand at 18.330 kb on - strand at 18.391 kb on + strand at 18.507 kb on + strand at 18.515 kb on - strand, within Dsui_0020 at 18.527 kb on + strand, within Dsui_0020 at 18.580 kb on - strand, within Dsui_0020 at 18.580 kb on - strand, within Dsui_0020 at 18.633 kb on + strand, within Dsui_0020 at 18.727 kb on + strand, within Dsui_0020 at 18.845 kb on + strand, within Dsui_0020 at 18.881 kb on - strand, within Dsui_0020 at 18.939 kb on + strand at 18.947 kb on - strand, within Dsui_0020 at 18.950 kb on + strand at 18.988 kb on + strand at 18.988 kb on + strand at 19.032 kb on + strand at 19.059 kb on - strand at 19.095 kb on + strand, within Dsui_0021 at 19.102 kb on - strand, within Dsui_0021 at 19.286 kb on - strand at 19.296 kb on - strand at 19.301 kb on - strand at 19.343 kb on + strand at 19.353 kb on - strand at 19.368 kb on + strand at 19.434 kb on - strand at 19.434 kb on - strand at 19.434 kb on - strand at 19.531 kb on + strand, within Dsui_0022 at 19.584 kb on + strand, within Dsui_0022 at 19.592 kb on - strand at 19.700 kb on + strand, within Dsui_0022 at 19.702 kb on - strand, within Dsui_0022 at 19.723 kb on + strand, within Dsui_0022 at 19.775 kb on - strand at 19.818 kb on + strand, within Dsui_0022 at 19.897 kb on + strand, within Dsui_0022 at 19.905 kb on - strand, within Dsui_0022 at 19.981 kb on + strand, within Dsui_0022 at 20.059 kb on - strand at 20.086 kb on - strand, within Dsui_0022 at 20.105 kb on - strand, within Dsui_0022 at 20.145 kb on - strand, within Dsui_0022 at 20.177 kb on - strand at 20.293 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 18,098 + +0.6 18,120 + +0.4 18,145 + +0.4 18,146 - +0.8 18,151 + -0.8 18,216 + +0.8 18,224 - +0.8 18,231 - +0.8 18,231 - +0.8 18,233 + -0.8 18,233 + -0.8 18,278 + +0.8 18,284 + -2.8 18,290 - +1.8 18,330 - -2.5 18,391 + -0.2 18,507 + +0.8 18,515 - Dsui_0020 0.31 -0.2 18,527 + Dsui_0020 0.32 -0.8 18,580 - Dsui_0020 0.39 +1.1 18,580 - Dsui_0020 0.39 -0.0 18,633 + Dsui_0020 0.46 -1.2 18,727 + Dsui_0020 0.57 -1.2 18,845 + Dsui_0020 0.72 -0.8 18,881 - Dsui_0020 0.77 -0.5 18,939 + +0.8 18,947 - Dsui_0020 0.85 +1.8 18,950 + -0.8 18,988 + -0.8 18,988 + +0.8 19,032 + +0.8 19,059 - -0.2 19,095 + Dsui_0021 0.15 -1.2 19,102 - Dsui_0021 0.18 +0.5 19,286 - +1.3 19,296 - +0.4 19,301 - -0.1 19,343 + +1.8 19,353 - +1.8 19,368 + -0.9 19,434 - -1.2 19,434 - -1.2 19,434 - -2.7 19,531 + Dsui_0022 0.14 -0.2 19,584 + Dsui_0022 0.19 -0.2 19,592 - +0.8 19,700 + Dsui_0022 0.32 +0.8 19,702 - Dsui_0022 0.32 -2.2 19,723 + Dsui_0022 0.34 +0.8 19,775 - +0.8 19,818 + Dsui_0022 0.44 -0.0 19,897 + Dsui_0022 0.53 -0.8 19,905 - Dsui_0022 0.53 +0.8 19,981 + Dsui_0022 0.62 +0.1 20,059 - +0.8 20,086 - Dsui_0022 0.73 -1.2 20,105 - Dsui_0022 0.75 -0.8 20,145 - Dsui_0022 0.79 -0.2 20,177 - +0.8 20,293 - +0.8
Or see this region's nucleotide sequence