Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0016 and Dsui_0017 are separated by 139 nucleotides Dsui_0017 and Dsui_0018 overlap by 1 nucleotides Dsui_0018 and Dsui_0019 are separated by 52 nucleotides
Dsui_0016: Dsui_0016 - transcriptional regulator, at 13,983 to 14,891
_0016
Dsui_0017: Dsui_0017 - 2-haloalkanoic acid dehalogenase, type II, at 15,031 to 15,720
_0017
Dsui_0018: Dsui_0018 - putative permease, at 15,720 to 16,505
_0018
Dsui_0019: Dsui_0019 - malate synthase A, at 16,558 to 18,156
_0019
Position (kb) 15
16 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 14.245 kb on - strand, within Dsui_0016 at 14.266 kb on - strand, within Dsui_0016 at 14.346 kb on + strand at 14.370 kb on - strand, within Dsui_0016 at 14.396 kb on + strand, within Dsui_0016 at 14.423 kb on - strand, within Dsui_0016 at 14.521 kb on - strand, within Dsui_0016 at 14.539 kb on - strand, within Dsui_0016 at 14.606 kb on + strand, within Dsui_0016 at 14.673 kb on + strand, within Dsui_0016 at 14.708 kb on + strand, within Dsui_0016 at 14.769 kb on + strand at 14.776 kb on - strand, within Dsui_0016 at 14.776 kb on - strand, within Dsui_0016 at 14.866 kb on + strand at 14.866 kb on + strand at 15.009 kb on + strand at 15.017 kb on - strand at 15.046 kb on - strand at 15.048 kb on + strand at 15.092 kb on - strand at 15.215 kb on - strand, within Dsui_0017 at 15.218 kb on + strand, within Dsui_0017 at 15.239 kb on + strand, within Dsui_0017 at 15.246 kb on - strand, within Dsui_0017 at 15.299 kb on - strand at 15.319 kb on + strand, within Dsui_0017 at 15.365 kb on + strand, within Dsui_0017 at 15.365 kb on + strand, within Dsui_0017 at 15.365 kb on + strand, within Dsui_0017 at 15.365 kb on + strand, within Dsui_0017 at 15.373 kb on - strand, within Dsui_0017 at 15.373 kb on - strand, within Dsui_0017 at 15.384 kb on - strand, within Dsui_0017 at 15.398 kb on + strand, within Dsui_0017 at 15.510 kb on - strand, within Dsui_0017 at 15.513 kb on + strand, within Dsui_0017 at 15.535 kb on - strand, within Dsui_0017 at 15.553 kb on - strand, within Dsui_0017 at 15.654 kb on + strand at 15.667 kb on - strand at 15.692 kb on - strand at 15.918 kb on - strand, within Dsui_0018 at 15.931 kb on + strand, within Dsui_0018 at 15.940 kb on - strand, within Dsui_0018 at 16.033 kb on - strand, within Dsui_0018 at 16.276 kb on - strand at 16.319 kb on - strand, within Dsui_0018 at 16.359 kb on - strand, within Dsui_0018 at 16.448 kb on + strand at 16.467 kb on + strand at 16.487 kb on + strand at 16.544 kb on + strand at 16.544 kb on + strand at 16.548 kb on + strand at 16.552 kb on - strand at 16.562 kb on + strand at 16.562 kb on + strand at 16.564 kb on - strand at 16.570 kb on - strand at 16.595 kb on + strand at 16.659 kb on + strand at 16.695 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 14,245 - Dsui_0016 0.29 +0.3 14,266 - Dsui_0016 0.31 +1.2 14,346 + +0.8 14,370 - Dsui_0016 0.43 +0.8 14,396 + Dsui_0016 0.45 -0.8 14,423 - Dsui_0016 0.48 +0.3 14,521 - Dsui_0016 0.59 +0.2 14,539 - Dsui_0016 0.61 +0.8 14,606 + Dsui_0016 0.69 +0.8 14,673 + Dsui_0016 0.76 -0.2 14,708 + Dsui_0016 0.80 -0.2 14,769 + +0.8 14,776 - Dsui_0016 0.87 +0.3 14,776 - Dsui_0016 0.87 +0.3 14,866 + -0.2 14,866 + +0.8 15,009 + -2.0 15,017 - -1.5 15,046 - +0.8 15,048 + +0.8 15,092 - -0.2 15,215 - Dsui_0017 0.27 -1.8 15,218 + Dsui_0017 0.27 +1.2 15,239 + Dsui_0017 0.30 +0.6 15,246 - Dsui_0017 0.31 +0.8 15,299 - +0.8 15,319 + Dsui_0017 0.42 +0.1 15,365 + Dsui_0017 0.48 +1.4 15,365 + Dsui_0017 0.48 -0.3 15,365 + Dsui_0017 0.48 -0.1 15,365 + Dsui_0017 0.48 +0.2 15,373 - Dsui_0017 0.50 -0.8 15,373 - Dsui_0017 0.50 -1.5 15,384 - Dsui_0017 0.51 -0.2 15,398 + Dsui_0017 0.53 +0.2 15,510 - Dsui_0017 0.69 -1.2 15,513 + Dsui_0017 0.70 +0.6 15,535 - Dsui_0017 0.73 -0.2 15,553 - Dsui_0017 0.76 -2.7 15,654 + -1.2 15,667 - +0.8 15,692 - -1.2 15,918 - Dsui_0018 0.25 +0.8 15,931 + Dsui_0018 0.27 -2.8 15,940 - Dsui_0018 0.28 -0.2 16,033 - Dsui_0018 0.40 -1.5 16,276 - +0.8 16,319 - Dsui_0018 0.76 +0.8 16,359 - Dsui_0018 0.81 +0.8 16,448 + -1.2 16,467 + +4.0 16,487 + +0.8 16,544 + -0.2 16,544 + -0.4 16,548 + +1.0 16,552 - +0.8 16,562 + +0.2 16,562 + +0.3 16,564 - +0.8 16,570 - -0.2 16,595 + +0.4 16,659 + +0.4 16,695 - +0.8
Or see this region's nucleotide sequence