Experiment: ALP with Fusidic 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0014 and Dsui_0015 are separated by 134 nucleotides Dsui_0015 and Dsui_0016 are separated by 39 nucleotides Dsui_0016 and Dsui_0017 are separated by 139 nucleotides Dsui_0017 and Dsui_0018 overlap by 1 nucleotides
Dsui_0014: Dsui_0014 - putative membrane protein, at 12,763 to 13,377
_0014
Dsui_0015: Dsui_0015 - putative signal-transduction protein containing cAMP-binding and CBS domains, at 13,512 to 13,943
_0015
Dsui_0016: Dsui_0016 - transcriptional regulator, at 13,983 to 14,891
_0016
Dsui_0017: Dsui_0017 - 2-haloalkanoic acid dehalogenase, type II, at 15,031 to 15,720
_0017
Dsui_0018: Dsui_0018 - putative permease, at 15,720 to 16,505
_0018
Position (kb) 13
14
15 Strain fitness (log2 ratio) -2
-1
0
1
2 at 13.011 kb on + strand, within Dsui_0014 at 13.040 kb on + strand, within Dsui_0014 at 13.040 kb on + strand, within Dsui_0014 at 13.048 kb on - strand at 13.048 kb on - strand, within Dsui_0014 at 13.127 kb on - strand at 13.223 kb on - strand, within Dsui_0014 at 13.225 kb on - strand at 13.238 kb on + strand, within Dsui_0014 at 13.253 kb on + strand, within Dsui_0014 at 13.289 kb on + strand, within Dsui_0014 at 13.297 kb on - strand, within Dsui_0014 at 13.308 kb on - strand, within Dsui_0014 at 13.357 kb on - strand at 13.457 kb on + strand at 13.510 kb on - strand at 13.522 kb on - strand at 13.525 kb on + strand at 13.525 kb on + strand at 13.598 kb on - strand, within Dsui_0015 at 13.609 kb on + strand, within Dsui_0015 at 13.678 kb on + strand, within Dsui_0015 at 13.678 kb on + strand at 13.696 kb on + strand at 13.786 kb on + strand, within Dsui_0015 at 13.786 kb on - strand at 13.853 kb on - strand at 13.935 kb on + strand at 14.245 kb on - strand, within Dsui_0016 at 14.266 kb on - strand, within Dsui_0016 at 14.346 kb on + strand at 14.370 kb on - strand, within Dsui_0016 at 14.396 kb on + strand, within Dsui_0016 at 14.423 kb on - strand, within Dsui_0016 at 14.521 kb on - strand, within Dsui_0016 at 14.539 kb on - strand, within Dsui_0016 at 14.606 kb on + strand, within Dsui_0016 at 14.673 kb on + strand, within Dsui_0016 at 14.708 kb on + strand, within Dsui_0016 at 14.769 kb on + strand at 14.776 kb on - strand, within Dsui_0016 at 14.776 kb on - strand, within Dsui_0016 at 14.866 kb on + strand at 14.866 kb on + strand at 15.009 kb on + strand at 15.017 kb on - strand at 15.046 kb on - strand at 15.048 kb on + strand at 15.092 kb on - strand at 15.215 kb on - strand, within Dsui_0017 at 15.218 kb on + strand, within Dsui_0017 at 15.239 kb on + strand, within Dsui_0017 at 15.246 kb on - strand, within Dsui_0017 at 15.299 kb on - strand at 15.319 kb on + strand, within Dsui_0017 at 15.365 kb on + strand, within Dsui_0017 at 15.365 kb on + strand, within Dsui_0017 at 15.365 kb on + strand, within Dsui_0017 at 15.365 kb on + strand, within Dsui_0017 at 15.373 kb on - strand, within Dsui_0017 at 15.373 kb on - strand, within Dsui_0017 at 15.384 kb on - strand, within Dsui_0017 at 15.398 kb on + strand, within Dsui_0017 at 15.510 kb on - strand, within Dsui_0017 at 15.513 kb on + strand, within Dsui_0017 at 15.535 kb on - strand, within Dsui_0017 at 15.553 kb on - strand, within Dsui_0017 at 15.654 kb on + strand at 15.667 kb on - strand at 15.692 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.02 mg/ml remove 13,011 + Dsui_0014 0.40 +2.6 13,040 + Dsui_0014 0.45 +2.3 13,040 + Dsui_0014 0.45 +2.4 13,048 - -0.2 13,048 - Dsui_0014 0.46 -0.2 13,127 - +0.8 13,223 - Dsui_0014 0.75 -0.2 13,225 - +0.8 13,238 + Dsui_0014 0.77 +2.2 13,253 + Dsui_0014 0.80 +2.0 13,289 + Dsui_0014 0.86 +2.4 13,297 - Dsui_0014 0.87 -1.2 13,308 - Dsui_0014 0.89 -0.8 13,357 - +0.8 13,457 + +0.8 13,510 - +0.8 13,522 - -0.8 13,525 + -0.8 13,525 + +0.8 13,598 - Dsui_0015 0.20 -0.2 13,609 + Dsui_0015 0.22 +0.8 13,678 + Dsui_0015 0.38 -0.2 13,678 + +0.8 13,696 + -0.2 13,786 + Dsui_0015 0.63 -1.5 13,786 - +0.8 13,853 - -0.2 13,935 + -1.2 14,245 - Dsui_0016 0.29 +0.3 14,266 - Dsui_0016 0.31 +1.2 14,346 + +0.8 14,370 - Dsui_0016 0.43 +0.8 14,396 + Dsui_0016 0.45 -0.8 14,423 - Dsui_0016 0.48 +0.3 14,521 - Dsui_0016 0.59 +0.2 14,539 - Dsui_0016 0.61 +0.8 14,606 + Dsui_0016 0.69 +0.8 14,673 + Dsui_0016 0.76 -0.2 14,708 + Dsui_0016 0.80 -0.2 14,769 + +0.8 14,776 - Dsui_0016 0.87 +0.3 14,776 - Dsui_0016 0.87 +0.3 14,866 + -0.2 14,866 + +0.8 15,009 + -2.0 15,017 - -1.5 15,046 - +0.8 15,048 + +0.8 15,092 - -0.2 15,215 - Dsui_0017 0.27 -1.8 15,218 + Dsui_0017 0.27 +1.2 15,239 + Dsui_0017 0.30 +0.6 15,246 - Dsui_0017 0.31 +0.8 15,299 - +0.8 15,319 + Dsui_0017 0.42 +0.1 15,365 + Dsui_0017 0.48 +1.4 15,365 + Dsui_0017 0.48 -0.3 15,365 + Dsui_0017 0.48 -0.1 15,365 + Dsui_0017 0.48 +0.2 15,373 - Dsui_0017 0.50 -0.8 15,373 - Dsui_0017 0.50 -1.5 15,384 - Dsui_0017 0.51 -0.2 15,398 + Dsui_0017 0.53 +0.2 15,510 - Dsui_0017 0.69 -1.2 15,513 + Dsui_0017 0.70 +0.6 15,535 - Dsui_0017 0.73 -0.2 15,553 - Dsui_0017 0.76 -2.7 15,654 + -1.2 15,667 - +0.8 15,692 - -1.2
Or see this region's nucleotide sequence