Experiment: PYE 25C survival rep2 12 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00069 and CCNA_00070 are separated by 72 nucleotides CCNA_00070 and CCNA_00071 are separated by 4 nucleotides CCNA_00071 and CCNA_00072 are separated by 43 nucleotides
CCNA_00069: CCNA_00069 - O-sialoglycoprotein endopeptidase, at 70,801 to 71,904
_00069
CCNA_00070: CCNA_00070 - porphobilinogen deaminase, at 71,977 to 72,945
_00070
CCNA_00071: CCNA_00071 - uroporphyrinogen-III synthase, at 72,950 to 73,636
_00071
CCNA_00072: CCNA_00072 - hypothetical protein, at 73,680 to 74,576
_00072
Position (kb)
71
72
73 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 71.967 kb on + strand at 71.975 kb on - strand at 72.967 kb on + strand at 72.967 kb on + strand at 72.967 kb on + strand at 73.615 kb on + strand at 73.623 kb on - strand at 73.632 kb on - strand at 73.650 kb on - strand at 73.650 kb on - strand at 73.667 kb on - strand at 73.723 kb on + strand at 73.731 kb on + strand at 73.739 kb on - strand at 73.776 kb on + strand, within CCNA_00072 at 73.786 kb on + strand, within CCNA_00072 at 73.800 kb on + strand, within CCNA_00072 at 73.822 kb on + strand, within CCNA_00072 at 73.830 kb on - strand, within CCNA_00072 at 73.830 kb on - strand, within CCNA_00072 at 73.830 kb on - strand, within CCNA_00072 at 73.830 kb on - strand, within CCNA_00072 at 73.831 kb on - strand, within CCNA_00072 at 73.890 kb on - strand, within CCNA_00072
Per-strain Table
Position Strand Gene LocusTag Fraction PYE 25C survival rep2 12 days remove 71,967 + -1.7 71,975 - -0.2 72,967 + -0.5 72,967 + +0.5 72,967 + -0.1 73,615 + -0.4 73,623 - -1.1 73,632 - -0.4 73,650 - -0.7 73,650 - -1.2 73,667 - -1.4 73,723 + -1.7 73,731 + +0.4 73,739 - -2.3 73,776 + CCNA_00072 0.11 -2.0 73,786 + CCNA_00072 0.12 -1.8 73,800 + CCNA_00072 0.13 -2.0 73,822 + CCNA_00072 0.16 -1.4 73,830 - CCNA_00072 0.17 -2.5 73,830 - CCNA_00072 0.17 -0.9 73,830 - CCNA_00072 0.17 -1.5 73,830 - CCNA_00072 0.17 -1.3 73,831 - CCNA_00072 0.17 -0.9 73,890 - CCNA_00072 0.23 -3.7
Or see this region's nucleotide sequence