Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16235

Experiment: Cytidine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16225 and BWI76_RS16230 are separated by 36 nucleotidesBWI76_RS16230 and BWI76_RS16235 overlap by 4 nucleotidesBWI76_RS16235 and BWI76_RS16240 are separated by 990 nucleotides BWI76_RS16225: BWI76_RS16225 - arginine:ornithine antiporter, at 3,365,468 to 3,366,850 _RS16225 BWI76_RS16230: BWI76_RS16230 - dihydromonapterin reductase, at 3,366,887 to 3,367,612 _RS16230 BWI76_RS16235: BWI76_RS16235 - membrane protein, at 3,367,609 to 3,367,944 _RS16235 BWI76_RS16240: BWI76_RS16240 - anti-adapter protein IraM, at 3,368,935 to 3,369,282 _RS16240 Position (kb) 3367 3368Strain fitness (log2 ratio) -2 -1 0 1 2at 3367.111 kb on + strand, within BWI76_RS16230at 3367.342 kb on - strand, within BWI76_RS16230at 3367.527 kb on + strandat 3367.557 kb on - strandat 3367.647 kb on + strand, within BWI76_RS16235at 3367.654 kb on + strand, within BWI76_RS16235at 3367.816 kb on - strand, within BWI76_RS16235at 3368.773 kb on - strandat 3368.900 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cytidine (N)
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3,367,111 + BWI76_RS16230 0.31 +0.2
3,367,342 - BWI76_RS16230 0.63 +0.2
3,367,527 + +1.8
3,367,557 - +0.8
3,367,647 + BWI76_RS16235 0.11 -2.3
3,367,654 + BWI76_RS16235 0.13 -0.7
3,367,816 - BWI76_RS16235 0.62 -1.2
3,368,773 - -1.2
3,368,900 - +0.3

Or see this region's nucleotide sequence