Experiment: BHIS with Sulfaquinoxaline 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3214 and BT3215 are separated by 16 nucleotides BT3215 and BT3216 are separated by 7 nucleotides BT3216 and BT3217 are separated by 30 nucleotides BT3217 and BT3218 overlap by 4 nucleotides
BT3214: BT3214 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI ptt file), at 4,106,571 to 4,107,629
BT3214
BT3215: BT3215 - conserved hypothetical protein (NCBI ptt file), at 4,107,646 to 4,107,981
BT3215
BT3216: BT3216 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine (NCBI ptt file), at 4,107,989 to 4,108,471
BT3216
BT3217: BT3217 - hypothetical protein (NCBI ptt file), at 4,108,502 to 4,108,693
BT3217
BT3218: BT3218 - hypothetical protein (NCBI ptt file), at 4,108,690 to 4,108,923
BT3218
Position (kb)
4107
4108 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4107.684 kb on + strand, within BT3215 at 4107.684 kb on + strand at 4107.712 kb on + strand, within BT3215 at 4107.734 kb on + strand, within BT3215 at 4107.907 kb on + strand, within BT3215 at 4107.932 kb on + strand, within BT3215 at 4107.932 kb on + strand, within BT3215 at 4107.974 kb on + strand at 4107.974 kb on + strand at 4108.563 kb on - strand, within BT3217 at 4108.571 kb on + strand, within BT3217 at 4108.571 kb on + strand, within BT3217 at 4108.572 kb on - strand, within BT3217 at 4108.583 kb on - strand, within BT3217 at 4108.613 kb on + strand, within BT3217 at 4108.614 kb on - strand, within BT3217 at 4108.624 kb on - strand, within BT3217 at 4108.643 kb on + strand, within BT3217 at 4108.648 kb on - strand, within BT3217 at 4108.687 kb on + strand at 4108.694 kb on - strand at 4108.708 kb on - strand at 4108.729 kb on + strand, within BT3218 at 4108.756 kb on - strand, within BT3218 at 4108.756 kb on - strand, within BT3218 at 4108.756 kb on - strand, within BT3218 at 4108.787 kb on + strand, within BT3218 at 4108.788 kb on - strand, within BT3218 at 4108.868 kb on + strand, within BT3218 at 4108.877 kb on - strand, within BT3218 at 4108.877 kb on - strand, within BT3218 at 4108.908 kb on + strand at 4108.909 kb on - strand at 4108.911 kb on - strand at 4108.911 kb on - strand at 4108.917 kb on + strand at 4108.939 kb on - strand at 4108.958 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Sulfaquinoxaline 0.2 mM remove 4,107,684 + BT3215 0.11 -2.2 4,107,684 + +0.5 4,107,712 + BT3215 0.20 -0.5 4,107,734 + BT3215 0.26 -2.3 4,107,907 + BT3215 0.78 -1.3 4,107,932 + BT3215 0.85 -2.7 4,107,932 + BT3215 0.85 -0.8 4,107,974 + -1.4 4,107,974 + -1.1 4,108,563 - BT3217 0.32 -1.1 4,108,571 + BT3217 0.36 -0.9 4,108,571 + BT3217 0.36 -1.1 4,108,572 - BT3217 0.36 -0.2 4,108,583 - BT3217 0.42 -1.0 4,108,613 + BT3217 0.58 -0.5 4,108,614 - BT3217 0.58 +2.6 4,108,624 - BT3217 0.64 -0.9 4,108,643 + BT3217 0.73 -0.5 4,108,648 - BT3217 0.76 +0.0 4,108,687 + -0.9 4,108,694 - +0.0 4,108,708 - -1.5 4,108,729 + BT3218 0.17 +0.3 4,108,756 - BT3218 0.28 +0.2 4,108,756 - BT3218 0.28 -1.4 4,108,756 - BT3218 0.28 -0.6 4,108,787 + BT3218 0.41 -1.2 4,108,788 - BT3218 0.42 +1.0 4,108,868 + BT3218 0.76 -1.3 4,108,877 - BT3218 0.80 -0.5 4,108,877 - BT3218 0.80 -0.5 4,108,908 + -1.1 4,108,909 - -0.5 4,108,911 - -1.0 4,108,911 - +0.5 4,108,917 + -1.5 4,108,939 - +0.0 4,108,958 + -1.3
Or see this region's nucleotide sequence