Experiment: BHIS with Sulfaquinoxaline 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0442 and BT0443 are separated by 225 nucleotides BT0443 and BT0444 are separated by 11 nucleotides BT0444 and BT0445 are separated by 13 nucleotides
BT0442: BT0442 - glycerophosphoryl diester phosphodiesterase (NCBI ptt file), at 536,438 to 538,213
BT0442
BT0443: BT0443 - thermostable beta-glucosidase B (NCBI ptt file), at 538,439 to 538,756
BT0443
BT0444: BT0444 - hypothetical protein (NCBI ptt file), at 538,768 to 540,024
BT0444
BT0445: BT0445 - endoglucanase E precursor (EGE) (NCBI ptt file), at 540,038 to 541,138
BT0445
Position (kb) 538
539
540
541 Strain fitness (log2 ratio) -2
-1
0
1
2 at 537.915 kb on - strand, within BT0442 at 537.949 kb on + strand, within BT0442 at 538.043 kb on + strand at 538.044 kb on - strand at 538.058 kb on + strand at 538.063 kb on + strand at 538.230 kb on - strand at 538.260 kb on + strand at 538.264 kb on + strand at 538.267 kb on - strand at 538.366 kb on + strand at 538.403 kb on + strand at 538.459 kb on - strand at 538.470 kb on + strand at 538.534 kb on - strand, within BT0443 at 538.700 kb on - strand, within BT0443 at 538.761 kb on - strand at 538.761 kb on - strand at 538.802 kb on + strand at 538.948 kb on - strand, within BT0444 at 538.949 kb on + strand, within BT0444 at 538.961 kb on + strand, within BT0444 at 539.002 kb on - strand, within BT0444 at 539.026 kb on + strand, within BT0444 at 539.110 kb on + strand, within BT0444 at 539.173 kb on + strand, within BT0444 at 539.173 kb on + strand, within BT0444 at 539.273 kb on + strand, within BT0444 at 539.452 kb on + strand, within BT0444 at 539.453 kb on - strand, within BT0444 at 539.455 kb on + strand, within BT0444 at 539.491 kb on - strand, within BT0444 at 539.496 kb on - strand, within BT0444 at 539.513 kb on - strand, within BT0444 at 539.543 kb on - strand, within BT0444 at 539.667 kb on + strand, within BT0444 at 539.667 kb on + strand, within BT0444 at 539.671 kb on - strand, within BT0444 at 539.673 kb on - strand, within BT0444 at 539.673 kb on - strand, within BT0444 at 539.678 kb on + strand, within BT0444 at 539.715 kb on - strand, within BT0444 at 539.722 kb on - strand, within BT0444 at 539.743 kb on - strand, within BT0444 at 539.745 kb on + strand, within BT0444 at 539.746 kb on - strand, within BT0444 at 539.764 kb on + strand, within BT0444 at 539.765 kb on - strand, within BT0444 at 539.812 kb on + strand, within BT0444 at 539.823 kb on - strand, within BT0444 at 539.832 kb on + strand, within BT0444 at 539.833 kb on - strand, within BT0444 at 539.946 kb on + strand at 539.947 kb on - strand at 539.955 kb on + strand at 540.013 kb on - strand at 540.038 kb on - strand at 540.090 kb on + strand at 540.090 kb on + strand at 540.117 kb on - strand at 540.120 kb on - strand at 540.206 kb on + strand, within BT0445 at 540.242 kb on - strand, within BT0445 at 540.273 kb on - strand, within BT0445 at 540.273 kb on - strand, within BT0445 at 540.484 kb on + strand, within BT0445 at 540.518 kb on + strand, within BT0445 at 540.525 kb on + strand, within BT0445 at 540.525 kb on + strand, within BT0445 at 540.551 kb on + strand, within BT0445 at 540.552 kb on - strand, within BT0445 at 540.579 kb on + strand, within BT0445 at 540.584 kb on + strand, within BT0445 at 540.595 kb on + strand, within BT0445 at 540.651 kb on + strand, within BT0445 at 540.651 kb on + strand, within BT0445 at 540.736 kb on + strand, within BT0445 at 540.759 kb on - strand, within BT0445 at 540.811 kb on + strand, within BT0445 at 540.812 kb on - strand, within BT0445 at 540.813 kb on + strand, within BT0445 at 540.814 kb on - strand, within BT0445 at 540.814 kb on - strand, within BT0445 at 540.845 kb on + strand, within BT0445 at 540.847 kb on + strand, within BT0445 at 540.848 kb on - strand, within BT0445 at 540.855 kb on + strand, within BT0445 at 540.856 kb on - strand, within BT0445 at 540.858 kb on + strand, within BT0445 at 540.892 kb on + strand, within BT0445 at 540.893 kb on - strand, within BT0445 at 540.897 kb on + strand, within BT0445 at 540.897 kb on + strand, within BT0445 at 540.939 kb on - strand, within BT0445 at 540.980 kb on + strand, within BT0445 at 540.982 kb on + strand, within BT0445
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Sulfaquinoxaline 0.2 mM remove 537,915 - BT0442 0.83 -0.6 537,949 + BT0442 0.85 +1.4 538,043 + +0.3 538,044 - +0.3 538,058 + -1.5 538,063 + -0.4 538,230 - -0.1 538,260 + -1.8 538,264 + +1.2 538,267 - -0.7 538,366 + +0.6 538,403 + -1.3 538,459 - -1.8 538,470 + +1.2 538,534 - BT0443 0.30 +0.9 538,700 - BT0443 0.82 -0.1 538,761 - +0.9 538,761 - -1.4 538,802 + -1.7 538,948 - BT0444 0.14 -2.7 538,949 + BT0444 0.14 -0.5 538,961 + BT0444 0.15 +0.3 539,002 - BT0444 0.19 -1.1 539,026 + BT0444 0.21 -1.5 539,110 + BT0444 0.27 -1.5 539,173 + BT0444 0.32 -1.0 539,173 + BT0444 0.32 -0.9 539,273 + BT0444 0.40 +0.9 539,452 + BT0444 0.54 -0.1 539,453 - BT0444 0.54 -0.1 539,455 + BT0444 0.55 +0.9 539,491 - BT0444 0.58 -0.9 539,496 - BT0444 0.58 -1.7 539,513 - BT0444 0.59 -1.0 539,543 - BT0444 0.62 +0.9 539,667 + BT0444 0.72 -1.5 539,667 + BT0444 0.72 -1.3 539,671 - BT0444 0.72 -0.9 539,673 - BT0444 0.72 -0.7 539,673 - BT0444 0.72 -0.7 539,678 + BT0444 0.72 +0.9 539,715 - BT0444 0.75 -1.9 539,722 - BT0444 0.76 -0.1 539,743 - BT0444 0.78 +1.9 539,745 + BT0444 0.78 +0.9 539,746 - BT0444 0.78 -0.5 539,764 + BT0444 0.79 -1.8 539,765 - BT0444 0.79 -0.1 539,812 + BT0444 0.83 -0.1 539,823 - BT0444 0.84 -2.0 539,832 + BT0444 0.85 +0.9 539,833 - BT0444 0.85 -1.3 539,946 + -0.1 539,947 - -0.7 539,955 + +0.9 540,013 - -1.1 540,038 - +1.4 540,090 + -0.8 540,090 + -1.5 540,117 - +0.9 540,120 - -0.1 540,206 + BT0445 0.15 -0.6 540,242 - BT0445 0.19 -1.1 540,273 - BT0445 0.21 +0.2 540,273 - BT0445 0.21 +0.9 540,484 + BT0445 0.41 -0.9 540,518 + BT0445 0.44 -0.3 540,525 + BT0445 0.44 -1.2 540,525 + BT0445 0.44 -2.1 540,551 + BT0445 0.47 +0.9 540,552 - BT0445 0.47 -0.7 540,579 + BT0445 0.49 -0.5 540,584 + BT0445 0.50 +0.1 540,595 + BT0445 0.51 +0.1 540,651 + BT0445 0.56 -0.7 540,651 + BT0445 0.56 -2.1 540,736 + BT0445 0.63 -0.7 540,759 - BT0445 0.65 -1.8 540,811 + BT0445 0.70 +2.4 540,812 - BT0445 0.70 -1.6 540,813 + BT0445 0.70 -0.2 540,814 - BT0445 0.70 -0.7 540,814 - BT0445 0.70 -0.1 540,845 + BT0445 0.73 +0.9 540,847 + BT0445 0.73 -0.7 540,848 - BT0445 0.74 -0.4 540,855 + BT0445 0.74 -2.5 540,856 - BT0445 0.74 +0.9 540,858 + BT0445 0.74 -1.1 540,892 + BT0445 0.78 -0.7 540,893 - BT0445 0.78 -1.2 540,897 + BT0445 0.78 -0.9 540,897 + BT0445 0.78 +0.3 540,939 - BT0445 0.82 +1.4 540,980 + BT0445 0.86 +0.1 540,982 + BT0445 0.86 -1.3
Or see this region's nucleotide sequence