Experiment: Formate/Acetate-Thiosulfate (60/30-15 mM) + 0.1% Yeast Extract
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1562 and DVU1563 are separated by 254 nucleotides DVU1563 and DORF35977 are separated by 220 nucleotides DORF35977 and tRNA-Arg are separated by 114 nucleotides tRNA-Arg and tRNA-Arg are separated by 19 nucleotides tRNA-Arg and tRNA-Arg are separated by 32 nucleotides
DVU1562: DVU1562 - HAMP domain protein (TIGR), at 1,641,270 to 1,644,164
DVU1562
DVU1563: DVU1563 - sensory box histidine kinase/response regulator (TIGR), at 1,644,419 to 1,647,538
DVU1563
DORF35977: DORF35977 - no description, at 1,647,759 to 1,648,106
DORF35977
DVU_tRNA-Arg-1: tRNA-Arg - tRNA with anticodon ACG for Arg (VIMSS-AUTO), at 1,648,221 to 1,648,297
tRNA-Arg
DVU_tRNA-Arg-2: tRNA-Arg - tRNA with anticodon ACG for Arg (VIMSS-AUTO), at 1,648,317 to 1,648,393
tRNA-Arg
DVU_tRNA-Arg-3: tRNA-Arg - tRNA with anticodon ACG for Arg (VIMSS-AUTO), at 1,648,426 to 1,648,502
tRNA-Arg
Position (kb)
1644
1645
1646
1647
1648 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1643.506 kb on + strand, within DVU1562 at 1644.183 kb on + strand at 1644.325 kb on - strand at 1644.385 kb on + strand at 1644.399 kb on - strand at 1644.402 kb on + strand at 1644.410 kb on - strand at 1644.410 kb on - strand at 1644.410 kb on - strand at 1644.450 kb on + strand at 1644.566 kb on - strand at 1644.567 kb on - strand at 1644.624 kb on - strand at 1644.635 kb on + strand at 1644.738 kb on + strand, within DVU1563 at 1644.740 kb on + strand, within DVU1563 at 1644.808 kb on - strand, within DVU1563 at 1644.971 kb on - strand, within DVU1563 at 1644.976 kb on - strand, within DVU1563 at 1645.015 kb on - strand, within DVU1563 at 1645.015 kb on - strand, within DVU1563 at 1645.264 kb on - strand, within DVU1563 at 1645.304 kb on - strand, within DVU1563 at 1645.323 kb on + strand, within DVU1563 at 1645.328 kb on + strand, within DVU1563 at 1645.374 kb on - strand, within DVU1563 at 1645.459 kb on - strand, within DVU1563 at 1645.459 kb on - strand, within DVU1563 at 1645.619 kb on + strand, within DVU1563 at 1645.707 kb on + strand, within DVU1563 at 1645.738 kb on + strand, within DVU1563 at 1645.843 kb on - strand, within DVU1563 at 1645.882 kb on + strand, within DVU1563 at 1645.882 kb on + strand, within DVU1563 at 1645.889 kb on + strand, within DVU1563 at 1645.890 kb on - strand, within DVU1563 at 1645.897 kb on - strand, within DVU1563 at 1645.960 kb on + strand, within DVU1563 at 1646.008 kb on + strand, within DVU1563 at 1646.022 kb on - strand, within DVU1563 at 1646.104 kb on + strand, within DVU1563 at 1646.112 kb on - strand, within DVU1563 at 1646.161 kb on + strand, within DVU1563 at 1646.178 kb on + strand, within DVU1563 at 1646.240 kb on - strand, within DVU1563 at 1646.250 kb on - strand, within DVU1563 at 1646.287 kb on - strand, within DVU1563 at 1646.373 kb on - strand, within DVU1563 at 1646.430 kb on + strand, within DVU1563 at 1646.466 kb on - strand, within DVU1563 at 1646.497 kb on + strand, within DVU1563 at 1646.548 kb on + strand, within DVU1563 at 1646.650 kb on + strand, within DVU1563 at 1646.650 kb on + strand, within DVU1563 at 1646.658 kb on - strand, within DVU1563 at 1646.799 kb on + strand, within DVU1563 at 1646.799 kb on + strand, within DVU1563 at 1646.884 kb on - strand, within DVU1563 at 1647.028 kb on - strand, within DVU1563 at 1647.100 kb on + strand, within DVU1563 at 1647.118 kb on + strand, within DVU1563 at 1647.181 kb on + strand, within DVU1563 at 1647.200 kb on - strand, within DVU1563 at 1647.243 kb on - strand at 1647.326 kb on - strand at 1647.326 kb on - strand at 1647.326 kb on - strand at 1647.326 kb on - strand at 1647.575 kb on - strand at 1647.909 kb on + strand, within DORF35977 at 1647.917 kb on - strand, within DORF35977 at 1647.981 kb on - strand, within DORF35977 at 1648.038 kb on - strand, within DORF35977 at 1648.258 kb on - strand, within tRNA-Arg at 1648.258 kb on - strand, within tRNA-Arg
Per-strain Table
Position Strand Gene LocusTag Fraction Formate/Acetate-Thiosulfate (60/30-15 mM) + 0.1% Yeast Extract remove 1,643,506 + DVU1562 0.77 +0.9 1,644,183 + +1.1 1,644,325 - +2.1 1,644,385 + -0.3 1,644,399 - -0.3 1,644,402 + -0.4 1,644,410 - -0.5 1,644,410 - -0.5 1,644,410 - -0.2 1,644,450 + +0.1 1,644,566 - -0.1 1,644,567 - +0.1 1,644,624 - +1.1 1,644,635 + +0.1 1,644,738 + DVU1563 0.10 +1.4 1,644,740 + DVU1563 0.10 +0.2 1,644,808 - DVU1563 0.12 -0.5 1,644,971 - DVU1563 0.18 -0.9 1,644,976 - DVU1563 0.18 +0.0 1,645,015 - DVU1563 0.19 -0.9 1,645,015 - DVU1563 0.19 +0.6 1,645,264 - DVU1563 0.27 +2.1 1,645,304 - DVU1563 0.28 +0.4 1,645,323 + DVU1563 0.29 -0.7 1,645,328 + DVU1563 0.29 +1.1 1,645,374 - DVU1563 0.31 +2.1 1,645,459 - DVU1563 0.33 -0.2 1,645,459 - DVU1563 0.33 -1.1 1,645,619 + DVU1563 0.38 +0.1 1,645,707 + DVU1563 0.41 -1.4 1,645,738 + DVU1563 0.42 +0.6 1,645,843 - DVU1563 0.46 +0.4 1,645,882 + DVU1563 0.47 -0.5 1,645,882 + DVU1563 0.47 -0.2 1,645,889 + DVU1563 0.47 +0.0 1,645,890 - DVU1563 0.47 -0.2 1,645,897 - DVU1563 0.47 +0.8 1,645,960 + DVU1563 0.49 -0.4 1,646,008 + DVU1563 0.51 +0.7 1,646,022 - DVU1563 0.51 +3.6 1,646,104 + DVU1563 0.54 -0.9 1,646,112 - DVU1563 0.54 +0.8 1,646,161 + DVU1563 0.56 +0.2 1,646,178 + DVU1563 0.56 +0.3 1,646,240 - DVU1563 0.58 +0.2 1,646,250 - DVU1563 0.59 -0.6 1,646,287 - DVU1563 0.60 -0.2 1,646,373 - DVU1563 0.63 +2.1 1,646,430 + DVU1563 0.64 +3.6 1,646,466 - DVU1563 0.66 -0.8 1,646,497 + DVU1563 0.67 +0.9 1,646,548 + DVU1563 0.68 +2.1 1,646,650 + DVU1563 0.72 -0.2 1,646,650 + DVU1563 0.72 +3.1 1,646,658 - DVU1563 0.72 -0.7 1,646,799 + DVU1563 0.76 +0.6 1,646,799 + DVU1563 0.76 +2.1 1,646,884 - DVU1563 0.79 +0.6 1,647,028 - DVU1563 0.84 -0.7 1,647,100 + DVU1563 0.86 +1.1 1,647,118 + DVU1563 0.87 +2.1 1,647,181 + DVU1563 0.89 +0.1 1,647,200 - DVU1563 0.89 -0.2 1,647,243 - -0.2 1,647,326 - +0.1 1,647,326 - +0.1 1,647,326 - -0.4 1,647,326 - -0.7 1,647,575 - +0.1 1,647,909 + DORF35977 0.43 -1.1 1,647,917 - DORF35977 0.45 -0.7 1,647,981 - DORF35977 0.64 -0.5 1,648,038 - DORF35977 0.80 -0.4 1,648,258 - tRNA-Arg DVU_tRNA-Arg-1 0.48 +0.6 1,648,258 - tRNA-Arg DVU_tRNA-Arg-1 0.48 -0.3
Or see this region's nucleotide sequence