Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3494 and Dsui_3495 are separated by 83 nucleotides Dsui_3495 and Dsui_3496 overlap by 1 nucleotides Dsui_3496 and Dsui_3497 are separated by 15 nucleotides Dsui_3497 and Dsui_3498 overlap by 8 nucleotides
Dsui_3494: Dsui_3494 - (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate reductase, at 3,759,745 to 3,760,674
_3494
Dsui_3495: Dsui_3495 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 3,760,758 to 3,761,201
_3495
Dsui_3496: Dsui_3496 - sulfite oxidase-like oxidoreductase, at 3,761,201 to 3,761,761
_3496
Dsui_3497: Dsui_3497 - lipoprotein signal peptidase, at 3,761,777 to 3,762,277
_3497
Dsui_3498: Dsui_3498 - isoleucyl-tRNA synthetase, at 3,762,270 to 3,765,062
_3498
Position (kb)
3761
3762 Strain fitness (log2 ratio)
-1
0
1 at 3761.125 kb on - strand, within Dsui_3495 at 3761.179 kb on - strand at 3761.254 kb on + strand at 3761.327 kb on + strand at 3761.444 kb on + strand, within Dsui_3496 at 3761.462 kb on + strand at 3761.539 kb on - strand, within Dsui_3496 at 3761.539 kb on - strand, within Dsui_3496 at 3761.545 kb on - strand, within Dsui_3496 at 3761.551 kb on + strand at 3761.552 kb on + strand, within Dsui_3496 at 3761.702 kb on + strand, within Dsui_3496
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 3,761,125 - Dsui_3495 0.83 +1.5 3,761,179 - +1.5 3,761,254 + +0.6 3,761,327 + +0.6 3,761,444 + Dsui_3496 0.43 -1.7 3,761,462 + +0.6 3,761,539 - Dsui_3496 0.60 -0.1 3,761,539 - Dsui_3496 0.60 -0.3 3,761,545 - Dsui_3496 0.61 -0.2 3,761,551 + +0.6 3,761,552 + Dsui_3496 0.63 -1.0 3,761,702 + Dsui_3496 0.89 -1.0
Or see this region's nucleotide sequence