Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3212 and Dsui_3213 are separated by 330 nucleotides Dsui_3213 and Dsui_3214 are separated by 125 nucleotides
Dsui_3212: Dsui_3212 - acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, at 3,446,689 to 3,448,356
_3212
Dsui_3213: Dsui_3213 - CBS domain-containing protein, at 3,448,687 to 3,449,136
_3213
Dsui_3214: Dsui_3214 - ATP-dependent exonuclase V beta subunit, helicase and exonuclease domain-containing, at 3,449,262 to 3,452,603
_3214
Position (kb)
3448
3449
3450 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3447.770 kb on + strand at 3447.783 kb on - strand, within Dsui_3212 at 3447.799 kb on - strand, within Dsui_3212 at 3447.852 kb on + strand, within Dsui_3212 at 3447.860 kb on - strand, within Dsui_3212 at 3447.937 kb on + strand, within Dsui_3212 at 3447.945 kb on - strand, within Dsui_3212 at 3447.948 kb on + strand, within Dsui_3212 at 3447.956 kb on - strand, within Dsui_3212 at 3448.039 kb on - strand, within Dsui_3212 at 3448.056 kb on - strand, within Dsui_3212 at 3448.061 kb on - strand, within Dsui_3212 at 3448.084 kb on - strand, within Dsui_3212 at 3448.102 kb on - strand, within Dsui_3212 at 3448.120 kb on - strand, within Dsui_3212 at 3448.144 kb on - strand, within Dsui_3212 at 3448.202 kb on + strand at 3448.252 kb on - strand at 3448.254 kb on + strand at 3448.256 kb on + strand at 3448.259 kb on - strand at 3448.264 kb on - strand at 3448.366 kb on - strand at 3448.421 kb on - strand at 3448.472 kb on + strand at 3448.480 kb on - strand at 3448.480 kb on - strand at 3448.480 kb on - strand at 3448.480 kb on - strand at 3448.480 kb on - strand at 3448.603 kb on - strand at 3448.690 kb on + strand at 3448.809 kb on - strand, within Dsui_3213 at 3448.900 kb on - strand, within Dsui_3213 at 3449.065 kb on + strand at 3449.065 kb on + strand at 3449.087 kb on + strand at 3449.095 kb on - strand at 3449.095 kb on - strand at 3449.095 kb on - strand at 3449.095 kb on - strand at 3449.183 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 3,447,770 + -0.3 3,447,783 - Dsui_3212 0.66 +1.9 3,447,799 - Dsui_3212 0.67 +0.9 3,447,852 + Dsui_3212 0.70 -0.9 3,447,860 - Dsui_3212 0.70 +0.8 3,447,937 + Dsui_3212 0.75 -0.3 3,447,945 - Dsui_3212 0.75 +0.2 3,447,948 + Dsui_3212 0.75 -2.9 3,447,956 - Dsui_3212 0.76 +0.4 3,448,039 - Dsui_3212 0.81 +0.7 3,448,056 - Dsui_3212 0.82 +0.9 3,448,061 - Dsui_3212 0.82 +0.9 3,448,084 - Dsui_3212 0.84 +0.8 3,448,102 - Dsui_3212 0.85 +1.0 3,448,120 - Dsui_3212 0.86 +0.7 3,448,144 - Dsui_3212 0.87 +1.2 3,448,202 + -0.3 3,448,252 - +0.9 3,448,254 + -1.3 3,448,256 + -0.9 3,448,259 - +0.9 3,448,264 - +1.4 3,448,366 - +0.6 3,448,421 - +0.0 3,448,472 + -0.3 3,448,480 - -0.3 3,448,480 - +0.7 3,448,480 - +0.7 3,448,480 - +0.7 3,448,480 - +0.7 3,448,603 - -0.3 3,448,690 + -1.3 3,448,809 - Dsui_3213 0.27 +1.3 3,448,900 - Dsui_3213 0.47 +0.9 3,449,065 + +0.7 3,449,065 + -0.3 3,449,087 + -1.3 3,449,095 - +0.6 3,449,095 - +0.7 3,449,095 - +0.8 3,449,095 - +0.8 3,449,183 - +0.4
Or see this region's nucleotide sequence