Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3197 and Dsui_3198 are separated by 93 nucleotides Dsui_3198 and Dsui_3199 are separated by 142 nucleotides Dsui_3199 and Dsui_3200 overlap by 1 nucleotides Dsui_3200 and Dsui_3201 are separated by 20 nucleotides
Dsui_3197: Dsui_3197 - aspartate-semialdehyde dehydrogenase, gamma-proteobacterial, at 3,433,033 to 3,434,166
_3197
Dsui_3198: Dsui_3198 - 3-isopropylmalate dehydrogenase, at 3,434,260 to 3,435,324
_3198
Dsui_3199: Dsui_3199 - 3-isopropylmalate dehydratase, small subunit, at 3,435,467 to 3,436,105
_3199
Dsui_3200: Dsui_3200 - putative small secreted protein, at 3,436,105 to 3,436,227
_3200
Dsui_3201: Dsui_3201 - 3-isopropylmalate dehydratase, large subunit, at 3,436,248 to 3,437,654
_3201
Position (kb)
3434
3435
3436 Strain fitness (log2 ratio)
-1
0
1 at 3434.206 kb on - strand at 3434.222 kb on + strand at 3434.223 kb on + strand at 3434.231 kb on - strand at 3434.258 kb on + strand at 3435.349 kb on - strand at 3435.373 kb on + strand at 3435.381 kb on - strand at 3436.237 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 3,434,206 - +0.0 3,434,222 + +0.7 3,434,223 + +0.7 3,434,231 - -0.0 3,434,258 + +0.7 3,435,349 - +0.4 3,435,373 + -0.3 3,435,381 - +0.0 3,436,237 - +0.5
Or see this region's nucleotide sequence