Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3104 and Dsui_3105 are separated by 54 nucleotides Dsui_3105 and Dsui_3106 are separated by 291 nucleotides Dsui_3106 and Dsui_3107 overlap by 8 nucleotides
Dsui_3104: Dsui_3104 - PAS domain S-box/diguanylate cyclase (GGDEF) domain-containing protein, at 3,322,174 to 3,324,675
_3104
Dsui_3105: Dsui_3105 - CBS domain-containing protein, at 3,324,730 to 3,326,046
_3105
Dsui_3106: Dsui_3106 - hypothetical protein, at 3,326,338 to 3,326,718
_3106
Dsui_3107: Dsui_3107 - Zn-dependent hydrolase, glyoxylase, at 3,326,711 to 3,327,349
_3107
Position (kb)
3324
3325
3326
3327 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3323.749 kb on + strand, within Dsui_3104 at 3323.817 kb on - strand, within Dsui_3104 at 3323.825 kb on - strand, within Dsui_3104 at 3324.226 kb on - strand, within Dsui_3104 at 3324.242 kb on + strand, within Dsui_3104 at 3324.295 kb on - strand, within Dsui_3104 at 3324.302 kb on - strand, within Dsui_3104 at 3324.375 kb on - strand, within Dsui_3104 at 3324.432 kb on + strand at 3324.432 kb on + strand at 3324.440 kb on - strand at 3324.523 kb on - strand at 3324.609 kb on - strand at 3324.700 kb on + strand at 3324.871 kb on - strand, within Dsui_3105 at 3324.943 kb on - strand, within Dsui_3105 at 3325.164 kb on + strand, within Dsui_3105 at 3325.172 kb on - strand, within Dsui_3105 at 3325.175 kb on - strand, within Dsui_3105 at 3325.245 kb on - strand, within Dsui_3105 at 3325.247 kb on + strand, within Dsui_3105 at 3325.255 kb on - strand, within Dsui_3105 at 3325.255 kb on - strand, within Dsui_3105 at 3325.255 kb on - strand, within Dsui_3105 at 3325.257 kb on + strand, within Dsui_3105 at 3325.534 kb on - strand, within Dsui_3105 at 3325.736 kb on + strand, within Dsui_3105 at 3325.744 kb on - strand, within Dsui_3105 at 3325.791 kb on + strand, within Dsui_3105 at 3325.798 kb on + strand, within Dsui_3105 at 3325.799 kb on - strand, within Dsui_3105 at 3326.076 kb on + strand at 3326.084 kb on - strand at 3326.084 kb on - strand at 3326.168 kb on - strand at 3326.227 kb on - strand at 3326.270 kb on - strand at 3326.344 kb on - strand at 3326.513 kb on - strand, within Dsui_3106 at 3326.572 kb on + strand, within Dsui_3106 at 3326.581 kb on + strand at 3326.647 kb on - strand, within Dsui_3106 at 3326.903 kb on - strand, within Dsui_3107 at 3326.908 kb on + strand at 3326.912 kb on - strand, within Dsui_3107 at 3326.974 kb on - strand, within Dsui_3107 at 3327.039 kb on - strand, within Dsui_3107
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 3,323,749 + Dsui_3104 0.63 -0.1 3,323,817 - Dsui_3104 0.66 -0.3 3,323,825 - Dsui_3104 0.66 -1.3 3,324,226 - Dsui_3104 0.82 -0.3 3,324,242 + Dsui_3104 0.83 +0.3 3,324,295 - Dsui_3104 0.85 -0.3 3,324,302 - Dsui_3104 0.85 +0.7 3,324,375 - Dsui_3104 0.88 -2.5 3,324,432 + -0.6 3,324,432 + +0.2 3,324,440 - -0.9 3,324,523 - -0.9 3,324,609 - -1.3 3,324,700 + -0.5 3,324,871 - Dsui_3105 0.11 -2.7 3,324,943 - Dsui_3105 0.16 -2.7 3,325,164 + Dsui_3105 0.33 -1.3 3,325,172 - Dsui_3105 0.34 -2.4 3,325,175 - Dsui_3105 0.34 -2.5 3,325,245 - Dsui_3105 0.39 -4.3 3,325,247 + Dsui_3105 0.39 +0.7 3,325,255 - Dsui_3105 0.40 -3.7 3,325,255 - Dsui_3105 0.40 -3.1 3,325,255 - Dsui_3105 0.40 -3.7 3,325,257 + Dsui_3105 0.40 +0.7 3,325,534 - Dsui_3105 0.61 -2.9 3,325,736 + Dsui_3105 0.76 +0.7 3,325,744 - Dsui_3105 0.77 -2.4 3,325,791 + Dsui_3105 0.81 -0.3 3,325,798 + Dsui_3105 0.81 -1.6 3,325,799 - Dsui_3105 0.81 -2.2 3,326,076 + +0.7 3,326,084 - -2.3 3,326,084 - -1.8 3,326,168 - -2.1 3,326,227 - +1.3 3,326,270 - +0.0 3,326,344 - +1.2 3,326,513 - Dsui_3106 0.46 +0.1 3,326,572 + Dsui_3106 0.61 -1.6 3,326,581 + +0.7 3,326,647 - Dsui_3106 0.81 -0.3 3,326,903 - Dsui_3107 0.30 -0.1 3,326,908 + +0.7 3,326,912 - Dsui_3107 0.31 +0.3 3,326,974 - Dsui_3107 0.41 -1.2 3,327,039 - Dsui_3107 0.51 +0.8
Or see this region's nucleotide sequence