Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2969 and Dsui_2970 are separated by 13 nucleotides Dsui_2970 and Dsui_2971 overlap by 4 nucleotides Dsui_2971 and Dsui_2972 are separated by 106 nucleotides Dsui_2972 and Dsui_2973 are separated by 5 nucleotides
Dsui_2969: Dsui_2969 - uroporphyrin-III C-methyltransferase, at 3,175,599 to 3,176,366
_2969
Dsui_2970: Dsui_2970 - putative Fe-S oxidoreductase, at 3,176,380 to 3,176,670
_2970
Dsui_2971: Dsui_2971 - putative esterase of the alpha-beta hydrolase superfamily, at 3,176,667 to 3,177,560
_2971
Dsui_2972: Dsui_2972 - leucyl/phenylalanyl-tRNA--protein transferase, at 3,177,667 to 3,178,383
_2972
Dsui_2973: Dsui_2973 - putative arginyl-tRNA:protein arginylyltransferase, at 3,178,389 to 3,179,162
_2973
Position (kb)
3176
3177
3178 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3175.690 kb on + strand at 3175.713 kb on + strand, within Dsui_2969 at 3175.828 kb on + strand, within Dsui_2969 at 3175.975 kb on + strand, within Dsui_2969 at 3176.067 kb on + strand, within Dsui_2969 at 3176.102 kb on - strand, within Dsui_2969 at 3176.173 kb on + strand, within Dsui_2969 at 3176.253 kb on - strand, within Dsui_2969 at 3176.269 kb on + strand, within Dsui_2969 at 3176.406 kb on + strand at 3176.414 kb on - strand, within Dsui_2970 at 3176.436 kb on + strand, within Dsui_2970 at 3176.479 kb on - strand, within Dsui_2970 at 3176.526 kb on + strand, within Dsui_2970 at 3176.850 kb on + strand, within Dsui_2971 at 3176.858 kb on - strand, within Dsui_2971 at 3176.863 kb on + strand, within Dsui_2971 at 3176.935 kb on + strand at 3176.943 kb on - strand, within Dsui_2971 at 3177.060 kb on - strand, within Dsui_2971 at 3177.128 kb on + strand at 3177.198 kb on - strand, within Dsui_2971 at 3177.245 kb on + strand at 3177.245 kb on + strand, within Dsui_2971 at 3177.289 kb on + strand at 3177.403 kb on - strand, within Dsui_2971 at 3177.432 kb on - strand, within Dsui_2971 at 3177.632 kb on - strand at 3177.637 kb on - strand at 3177.643 kb on - strand at 3177.680 kb on - strand at 3177.834 kb on + strand, within Dsui_2972 at 3177.834 kb on - strand at 3177.839 kb on + strand, within Dsui_2972 at 3177.864 kb on - strand, within Dsui_2972 at 3177.922 kb on + strand, within Dsui_2972 at 3177.955 kb on - strand at 3178.163 kb on + strand, within Dsui_2972 at 3178.233 kb on + strand, within Dsui_2972 at 3178.241 kb on + strand, within Dsui_2972 at 3178.241 kb on + strand, within Dsui_2972 at 3178.441 kb on - strand at 3178.457 kb on - strand at 3178.486 kb on + strand, within Dsui_2973
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 3,175,690 + +0.8 3,175,713 + Dsui_2969 0.15 -1.2 3,175,828 + Dsui_2969 0.30 -0.5 3,175,975 + Dsui_2969 0.49 -0.2 3,176,067 + Dsui_2969 0.61 +1.6 3,176,102 - Dsui_2969 0.65 -1.5 3,176,173 + Dsui_2969 0.75 -1.2 3,176,253 - Dsui_2969 0.85 +0.8 3,176,269 + Dsui_2969 0.87 -2.0 3,176,406 + -1.5 3,176,414 - Dsui_2970 0.12 -1.9 3,176,436 + Dsui_2970 0.19 +1.8 3,176,479 - Dsui_2970 0.34 +0.2 3,176,526 + Dsui_2970 0.50 -0.8 3,176,850 + Dsui_2971 0.20 -0.2 3,176,858 - Dsui_2971 0.21 -0.9 3,176,863 + Dsui_2971 0.22 -0.2 3,176,935 + +0.8 3,176,943 - Dsui_2971 0.31 +0.6 3,177,060 - Dsui_2971 0.44 +0.8 3,177,128 + -1.5 3,177,198 - Dsui_2971 0.59 -1.2 3,177,245 + -0.2 3,177,245 + Dsui_2971 0.65 +0.8 3,177,289 + +0.8 3,177,403 - Dsui_2971 0.82 -0.5 3,177,432 - Dsui_2971 0.86 -1.2 3,177,632 - +0.8 3,177,637 - +0.8 3,177,643 - +0.8 3,177,680 - +0.8 3,177,834 + Dsui_2972 0.23 -1.5 3,177,834 - +0.8 3,177,839 + Dsui_2972 0.24 +0.8 3,177,864 - Dsui_2972 0.27 -0.8 3,177,922 + Dsui_2972 0.36 -0.2 3,177,955 - -0.8 3,178,163 + Dsui_2972 0.69 -0.2 3,178,233 + Dsui_2972 0.79 +0.3 3,178,241 + Dsui_2972 0.80 +0.0 3,178,241 + Dsui_2972 0.80 -2.7 3,178,441 - +0.8 3,178,457 - +0.8 3,178,486 + Dsui_2973 0.13 +0.7
Or see this region's nucleotide sequence