Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2696 and Dsui_2697 are separated by 57 nucleotides Dsui_2697 and Dsui_2698 are separated by 2 nucleotides Dsui_2698 and Dsui_2699 are separated by 51 nucleotides
Dsui_2696: Dsui_2696 - (protein-PII) uridylyltransferase, at 2,847,694 to 2,850,285
_2696
Dsui_2697: Dsui_2697 - peptide deformylase, at 2,850,343 to 2,850,891
_2697
Dsui_2698: Dsui_2698 - purine nucleoside phosphorylase, at 2,850,894 to 2,851,646
_2698
Dsui_2699: Dsui_2699 - hypoxanthine-guanine phosphoribosyltransferase, at 2,851,698 to 2,852,246
_2699
Position (kb)
2850
2851 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2849.376 kb on - strand, within Dsui_2696 at 2849.420 kb on + strand, within Dsui_2696 at 2849.428 kb on - strand, within Dsui_2696 at 2849.428 kb on - strand at 2849.463 kb on + strand, within Dsui_2696 at 2849.498 kb on - strand, within Dsui_2696 at 2849.584 kb on + strand, within Dsui_2696 at 2849.695 kb on + strand, within Dsui_2696 at 2849.772 kb on - strand, within Dsui_2696 at 2849.855 kb on - strand, within Dsui_2696 at 2849.890 kb on - strand, within Dsui_2696 at 2849.890 kb on - strand, within Dsui_2696 at 2849.952 kb on + strand, within Dsui_2696 at 2850.050 kb on - strand at 2850.124 kb on + strand at 2850.124 kb on + strand at 2850.221 kb on + strand at 2850.221 kb on + strand at 2850.281 kb on - strand at 2850.293 kb on - strand at 2850.299 kb on - strand at 2850.462 kb on + strand, within Dsui_2697 at 2850.470 kb on - strand, within Dsui_2697 at 2850.490 kb on - strand, within Dsui_2697 at 2850.584 kb on - strand, within Dsui_2697 at 2850.619 kb on + strand at 2850.674 kb on - strand, within Dsui_2697 at 2850.722 kb on - strand, within Dsui_2697 at 2850.821 kb on - strand, within Dsui_2697 at 2851.056 kb on - strand, within Dsui_2698 at 2851.114 kb on + strand, within Dsui_2698 at 2851.114 kb on + strand at 2851.132 kb on + strand, within Dsui_2698 at 2851.163 kb on + strand, within Dsui_2698 at 2851.182 kb on + strand, within Dsui_2698 at 2851.263 kb on + strand at 2851.517 kb on - strand, within Dsui_2698 at 2851.547 kb on + strand, within Dsui_2698 at 2851.614 kb on + strand at 2851.621 kb on - strand at 2851.622 kb on - strand at 2851.864 kb on + strand at 2851.864 kb on + strand, within Dsui_2699
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 2,849,376 - Dsui_2696 0.65 -0.3 2,849,420 + Dsui_2696 0.67 +1.3 2,849,428 - Dsui_2696 0.67 +0.7 2,849,428 - +0.7 2,849,463 + Dsui_2696 0.68 +1.3 2,849,498 - Dsui_2696 0.70 +0.7 2,849,584 + Dsui_2696 0.73 +0.8 2,849,695 + Dsui_2696 0.77 +1.3 2,849,772 - Dsui_2696 0.80 +0.7 2,849,855 - Dsui_2696 0.83 -1.2 2,849,890 - Dsui_2696 0.85 -1.2 2,849,890 - Dsui_2696 0.85 +0.8 2,849,952 + Dsui_2696 0.87 +0.9 2,850,050 - -2.4 2,850,124 + +1.1 2,850,124 + +1.9 2,850,221 + +1.1 2,850,221 + +1.6 2,850,281 - +0.8 2,850,293 - +0.8 2,850,299 - -1.8 2,850,462 + Dsui_2697 0.22 +0.8 2,850,470 - Dsui_2697 0.23 +0.6 2,850,490 - Dsui_2697 0.27 -0.6 2,850,584 - Dsui_2697 0.44 -0.2 2,850,619 + +0.8 2,850,674 - Dsui_2697 0.60 -0.3 2,850,722 - Dsui_2697 0.69 -0.3 2,850,821 - Dsui_2697 0.87 +0.5 2,851,056 - Dsui_2698 0.22 -0.1 2,851,114 + Dsui_2698 0.29 -0.8 2,851,114 + +0.8 2,851,132 + Dsui_2698 0.32 +0.8 2,851,163 + Dsui_2698 0.36 +0.8 2,851,182 + Dsui_2698 0.38 -0.2 2,851,263 + -0.8 2,851,517 - Dsui_2698 0.83 +0.1 2,851,547 + Dsui_2698 0.87 -1.6 2,851,614 + -1.2 2,851,621 - -0.5 2,851,622 - -0.8 2,851,864 + -0.8 2,851,864 + Dsui_2699 0.30 -0.8
Or see this region's nucleotide sequence