Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2405 and Dsui_2406 are separated by 199 nucleotides Dsui_2406 and Dsui_2407 are separated by 12 nucleotides Dsui_2407 and Dsui_2408 overlap by 1 nucleotides Dsui_2408 and Dsui_2409 are separated by 15 nucleotides
Dsui_2405: Dsui_2405 - uracil-DNA glycosylase, family 4, at 2,556,837 to 2,557,694
_2405
Dsui_2406: Dsui_2406 - hypothetical protein, at 2,557,894 to 2,558,496
_2406
Dsui_2407: Dsui_2407 - beta-propeller domain-containing protein, methanol dehydrogenase, at 2,558,509 to 2,559,339
_2407
Dsui_2408: Dsui_2408 - putative membrane protein, at 2,559,339 to 2,559,845
_2408
Dsui_2409: Dsui_2409 - Major Facilitator Superfamily transporter, at 2,559,861 to 2,561,126
_2409
Position (kb)
2558
2559
2560 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2557.699 kb on + strand at 2557.896 kb on - strand at 2557.896 kb on - strand at 2557.938 kb on + strand at 2557.941 kb on - strand at 2557.946 kb on - strand at 2557.976 kb on - strand at 2557.983 kb on + strand, within Dsui_2406 at 2557.983 kb on + strand, within Dsui_2406 at 2557.983 kb on + strand, within Dsui_2406 at 2557.983 kb on + strand, within Dsui_2406 at 2557.991 kb on - strand at 2557.991 kb on - strand at 2557.991 kb on - strand, within Dsui_2406 at 2558.064 kb on + strand, within Dsui_2406 at 2558.067 kb on - strand at 2558.072 kb on - strand at 2558.177 kb on + strand, within Dsui_2406 at 2558.195 kb on - strand at 2558.238 kb on + strand, within Dsui_2406 at 2558.243 kb on + strand, within Dsui_2406 at 2558.288 kb on + strand, within Dsui_2406 at 2558.420 kb on - strand, within Dsui_2406 at 2558.445 kb on + strand at 2558.448 kb on + strand at 2558.738 kb on + strand at 2558.745 kb on + strand, within Dsui_2407 at 2558.753 kb on + strand at 2558.791 kb on + strand, within Dsui_2407 at 2559.041 kb on + strand, within Dsui_2407 at 2559.041 kb on + strand, within Dsui_2407 at 2559.178 kb on - strand, within Dsui_2407 at 2559.208 kb on + strand, within Dsui_2407 at 2559.208 kb on - strand at 2559.451 kb on + strand, within Dsui_2408 at 2559.454 kb on + strand, within Dsui_2408 at 2559.512 kb on - strand, within Dsui_2408 at 2559.512 kb on - strand, within Dsui_2408 at 2559.548 kb on - strand, within Dsui_2408 at 2559.555 kb on - strand, within Dsui_2408 at 2559.593 kb on - strand, within Dsui_2408 at 2559.593 kb on - strand, within Dsui_2408 at 2559.593 kb on - strand at 2559.593 kb on - strand, within Dsui_2408 at 2559.610 kb on + strand, within Dsui_2408 at 2559.610 kb on + strand, within Dsui_2408 at 2559.610 kb on + strand, within Dsui_2408 at 2559.618 kb on - strand at 2559.618 kb on - strand, within Dsui_2408 at 2559.618 kb on - strand at 2559.618 kb on - strand, within Dsui_2408 at 2559.676 kb on - strand, within Dsui_2408 at 2559.703 kb on + strand, within Dsui_2408 at 2559.703 kb on + strand, within Dsui_2408 at 2559.772 kb on - strand, within Dsui_2408 at 2559.790 kb on + strand, within Dsui_2408 at 2559.849 kb on - strand at 2559.862 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 2,557,699 + -0.3 2,557,896 - +0.7 2,557,896 - -0.3 2,557,938 + +3.7 2,557,941 - -0.3 2,557,946 - +0.7 2,557,976 - -0.3 2,557,983 + Dsui_2406 0.15 +2.7 2,557,983 + Dsui_2406 0.15 +0.1 2,557,983 + Dsui_2406 0.15 -0.9 2,557,983 + Dsui_2406 0.15 -0.3 2,557,991 - +0.7 2,557,991 - -2.1 2,557,991 - Dsui_2406 0.16 +0.7 2,558,064 + Dsui_2406 0.28 +0.4 2,558,067 - -1.3 2,558,072 - -0.3 2,558,177 + Dsui_2406 0.47 -0.3 2,558,195 - -0.9 2,558,238 + Dsui_2406 0.57 -0.2 2,558,243 + Dsui_2406 0.58 -1.1 2,558,288 + Dsui_2406 0.65 -1.7 2,558,420 - Dsui_2406 0.87 -1.3 2,558,445 + -0.8 2,558,448 + -1.9 2,558,738 + -0.9 2,558,745 + Dsui_2407 0.28 +0.4 2,558,753 + -0.3 2,558,791 + Dsui_2407 0.34 +0.5 2,559,041 + Dsui_2407 0.64 +0.7 2,559,041 + Dsui_2407 0.64 -0.1 2,559,178 - Dsui_2407 0.81 -1.6 2,559,208 + Dsui_2407 0.84 -0.8 2,559,208 - -1.3 2,559,451 + Dsui_2408 0.22 -1.6 2,559,454 + Dsui_2408 0.23 +0.0 2,559,512 - Dsui_2408 0.34 -1.3 2,559,512 - Dsui_2408 0.34 -2.9 2,559,548 - Dsui_2408 0.41 -2.3 2,559,555 - Dsui_2408 0.43 -0.9 2,559,593 - Dsui_2408 0.50 -0.3 2,559,593 - Dsui_2408 0.50 +0.7 2,559,593 - +0.7 2,559,593 - Dsui_2408 0.50 -0.9 2,559,610 + Dsui_2408 0.53 -0.1 2,559,610 + Dsui_2408 0.53 -1.6 2,559,610 + Dsui_2408 0.53 -1.2 2,559,618 - -0.3 2,559,618 - Dsui_2408 0.55 +0.7 2,559,618 - +0.7 2,559,618 - Dsui_2408 0.55 +0.7 2,559,676 - Dsui_2408 0.66 -3.1 2,559,703 + Dsui_2408 0.72 -0.7 2,559,703 + Dsui_2408 0.72 -0.5 2,559,772 - Dsui_2408 0.85 +0.2 2,559,790 + Dsui_2408 0.89 -1.6 2,559,849 - +0.5 2,559,862 - +1.3
Or see this region's nucleotide sequence