Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2385 and Dsui_2386 are separated by 111 nucleotides Dsui_2386 and Dsui_2387 overlap by 4 nucleotides Dsui_2387 and Dsui_2388 overlap by 14 nucleotides
Dsui_2385: Dsui_2385 - transcription-repair coupling factor Mfd, at 2,535,212 to 2,538,808
_2385
Dsui_2386: Dsui_2386 - pyruvate-formate lyase-activating enzyme, at 2,538,920 to 2,540,020
_2386
Dsui_2387: Dsui_2387 - hypothetical protein, at 2,540,017 to 2,540,622
_2387
Dsui_2388: Dsui_2388 - putative dioxygenase, at 2,540,609 to 2,541,415
_2388
Position (kb)
2538
2539
2540
2541 Strain fitness (log2 ratio)
-2
-1
0
1 at 2538.098 kb on - strand, within Dsui_2385 at 2538.103 kb on - strand, within Dsui_2385 at 2538.121 kb on + strand at 2538.128 kb on - strand, within Dsui_2385 at 2538.370 kb on - strand, within Dsui_2385 at 2538.556 kb on - strand at 2538.648 kb on + strand at 2539.039 kb on + strand at 2539.047 kb on - strand, within Dsui_2386 at 2539.047 kb on - strand, within Dsui_2386 at 2539.047 kb on - strand at 2539.048 kb on + strand at 2539.098 kb on - strand, within Dsui_2386 at 2539.320 kb on - strand at 2539.331 kb on - strand at 2539.403 kb on - strand at 2539.451 kb on - strand at 2539.451 kb on - strand at 2539.625 kb on - strand, within Dsui_2386 at 2539.632 kb on - strand, within Dsui_2386 at 2539.632 kb on - strand, within Dsui_2386 at 2539.770 kb on - strand, within Dsui_2386 at 2539.786 kb on + strand at 2540.388 kb on - strand, within Dsui_2387 at 2540.559 kb on - strand at 2540.610 kb on + strand at 2540.637 kb on - strand at 2540.855 kb on - strand, within Dsui_2388 at 2540.979 kb on - strand, within Dsui_2388
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 2,538,098 - Dsui_2385 0.80 +0.1 2,538,103 - Dsui_2385 0.80 -0.7 2,538,121 + +0.7 2,538,128 - Dsui_2385 0.81 +0.6 2,538,370 - Dsui_2385 0.88 +0.1 2,538,556 - -0.8 2,538,648 + -1.3 2,539,039 + -0.9 2,539,047 - Dsui_2386 0.12 -0.3 2,539,047 - Dsui_2386 0.12 -0.9 2,539,047 - +0.7 2,539,048 + -0.9 2,539,098 - Dsui_2386 0.16 -1.3 2,539,320 - +0.7 2,539,331 - -0.9 2,539,403 - +0.7 2,539,451 - +0.7 2,539,451 - +0.7 2,539,625 - Dsui_2386 0.64 +0.7 2,539,632 - Dsui_2386 0.65 -0.3 2,539,632 - Dsui_2386 0.65 -0.9 2,539,770 - Dsui_2386 0.77 -1.9 2,539,786 + +0.7 2,540,388 - Dsui_2387 0.61 +0.7 2,540,559 - -0.9 2,540,610 + +0.7 2,540,637 - -1.3 2,540,855 - Dsui_2388 0.30 -2.6 2,540,979 - Dsui_2388 0.46 -0.3
Or see this region's nucleotide sequence