Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2183 and Dsui_2184 are separated by 114 nucleotides Dsui_2184 and Dsui_2185 are separated by 71 nucleotides Dsui_2185 and Dsui_2186 are separated by 185 nucleotides Dsui_2186 and Dsui_2187 are separated by 70 nucleotides
Dsui_2183: Dsui_2183 - aldose 1-epimerase-like enzyme, at 2,318,747 to 2,319,619
_2183
Dsui_2184: Dsui_2184 - hypothetical protein, at 2,319,734 to 2,320,282
_2184
Dsui_2185: Dsui_2185 - DNA-3-methyladenine glycosylase I, at 2,320,354 to 2,320,926
_2185
Dsui_2186: Dsui_2186 - Protein of unknown function (DUF3567), at 2,321,112 to 2,321,345
_2186
Dsui_2187: Dsui_2187 - acyl-CoA synthetase/AMP-acid ligase, at 2,321,416 to 2,323,113
_2187
Position (kb)
2320
2321 Strain fitness (log2 ratio)
-2
-1
0
1 at 2319.369 kb on - strand, within Dsui_2183 at 2319.402 kb on - strand, within Dsui_2183 at 2319.564 kb on - strand at 2319.874 kb on + strand at 2319.977 kb on + strand, within Dsui_2184 at 2320.260 kb on - strand at 2320.299 kb on - strand at 2320.402 kb on + strand at 2320.425 kb on + strand, within Dsui_2185 at 2320.477 kb on - strand at 2320.619 kb on - strand, within Dsui_2185 at 2320.650 kb on + strand at 2320.697 kb on + strand at 2320.784 kb on + strand, within Dsui_2185 at 2320.901 kb on - strand at 2320.978 kb on + strand at 2321.011 kb on + strand at 2321.018 kb on - strand at 2321.045 kb on - strand at 2321.155 kb on + strand, within Dsui_2186 at 2321.317 kb on + strand, within Dsui_2186 at 2321.353 kb on - strand at 2321.435 kb on - strand at 2321.534 kb on + strand at 2321.542 kb on - strand at 2321.542 kb on - strand at 2321.660 kb on - strand at 2321.833 kb on - strand at 2321.852 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 2,319,369 - Dsui_2183 0.71 -1.5 2,319,402 - Dsui_2183 0.75 +0.9 2,319,564 - -1.1 2,319,874 + +0.9 2,319,977 + Dsui_2184 0.44 -0.1 2,320,260 - -1.9 2,320,299 - -1.1 2,320,402 + -0.1 2,320,425 + Dsui_2185 0.12 -0.7 2,320,477 - +0.9 2,320,619 - Dsui_2185 0.46 -0.1 2,320,650 + +0.9 2,320,697 + +0.9 2,320,784 + Dsui_2185 0.75 -0.7 2,320,901 - +0.9 2,320,978 + +0.9 2,321,011 + +0.9 2,321,018 - +0.9 2,321,045 - -0.1 2,321,155 + Dsui_2186 0.18 +0.9 2,321,317 + Dsui_2186 0.88 -0.1 2,321,353 - +0.9 2,321,435 - -1.1 2,321,534 + +0.9 2,321,542 - -1.1 2,321,542 - -0.1 2,321,660 - -0.7 2,321,833 - +0.9 2,321,852 + +0.9
Or see this region's nucleotide sequence