Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2048 and Dsui_2049 are separated by 93 nucleotides Dsui_2049 and Dsui_2050 are separated by 59 nucleotides Dsui_2050 and Dsui_2051 are separated by 270 nucleotides Dsui_2051 and Dsui_2052 overlap by 4 nucleotides
Dsui_2048: Dsui_2048 - ATPase component of ABC transporters with duplicated ATPase domain, at 2,168,464 to 2,170,146
_2048
Dsui_2049: Dsui_2049 - ABC-type uncharacterized transport system, permease and ATPase component, at 2,170,240 to 2,171,943
_2049
Dsui_2050: Dsui_2050 - hypothetical protein, at 2,172,003 to 2,172,377
_2050
Dsui_2051: Dsui_2051 - hypothetical protein, at 2,172,648 to 2,172,818
_2051
Dsui_2052: Dsui_2052 - hypothetical protein, at 2,172,815 to 2,173,012
_2052
Position (kb)
2170
2171
2172 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2169.273 kb on - strand, within Dsui_2048 at 2169.302 kb on + strand, within Dsui_2048 at 2169.302 kb on + strand, within Dsui_2048 at 2169.352 kb on - strand, within Dsui_2048 at 2169.364 kb on + strand, within Dsui_2048 at 2169.370 kb on - strand, within Dsui_2048 at 2169.372 kb on - strand, within Dsui_2048 at 2169.439 kb on - strand, within Dsui_2048 at 2169.476 kb on + strand, within Dsui_2048 at 2169.592 kb on - strand, within Dsui_2048 at 2169.816 kb on - strand, within Dsui_2048 at 2170.001 kb on + strand at 2170.131 kb on + strand at 2170.339 kb on - strand at 2170.375 kb on - strand at 2170.397 kb on - strand at 2170.453 kb on + strand, within Dsui_2049 at 2170.483 kb on - strand at 2170.616 kb on + strand at 2170.617 kb on + strand, within Dsui_2049 at 2170.617 kb on + strand, within Dsui_2049 at 2170.666 kb on - strand, within Dsui_2049 at 2170.685 kb on - strand, within Dsui_2049 at 2170.863 kb on + strand, within Dsui_2049 at 2170.996 kb on + strand at 2171.004 kb on - strand, within Dsui_2049 at 2171.128 kb on - strand, within Dsui_2049 at 2171.130 kb on + strand, within Dsui_2049 at 2171.130 kb on + strand, within Dsui_2049 at 2171.138 kb on - strand, within Dsui_2049 at 2171.138 kb on - strand, within Dsui_2049 at 2171.138 kb on - strand, within Dsui_2049 at 2171.153 kb on - strand, within Dsui_2049 at 2171.155 kb on - strand, within Dsui_2049 at 2171.166 kb on + strand, within Dsui_2049 at 2171.168 kb on - strand, within Dsui_2049 at 2171.174 kb on - strand, within Dsui_2049 at 2171.194 kb on - strand, within Dsui_2049 at 2171.214 kb on - strand, within Dsui_2049 at 2171.232 kb on - strand, within Dsui_2049 at 2171.270 kb on - strand at 2171.361 kb on - strand, within Dsui_2049 at 2171.390 kb on + strand, within Dsui_2049 at 2171.410 kb on - strand, within Dsui_2049 at 2171.480 kb on + strand at 2171.525 kb on - strand, within Dsui_2049 at 2171.578 kb on - strand at 2171.670 kb on + strand, within Dsui_2049 at 2171.730 kb on - strand, within Dsui_2049 at 2171.805 kb on + strand at 2171.822 kb on + strand at 2171.850 kb on + strand at 2172.045 kb on - strand, within Dsui_2050 at 2172.106 kb on + strand, within Dsui_2050 at 2172.111 kb on + strand at 2172.114 kb on - strand, within Dsui_2050 at 2172.114 kb on - strand, within Dsui_2050 at 2172.119 kb on - strand, within Dsui_2050 at 2172.217 kb on - strand, within Dsui_2050 at 2172.386 kb on - strand at 2172.405 kb on - strand at 2172.667 kb on + strand, within Dsui_2051 at 2172.677 kb on + strand at 2172.729 kb on - strand, within Dsui_2051 at 2172.831 kb on + strand at 2172.908 kb on + strand, within Dsui_2052 at 2172.931 kb on - strand, within Dsui_2052
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 2,169,273 - Dsui_2048 0.48 +0.9 2,169,302 + Dsui_2048 0.50 -1.9 2,169,302 + Dsui_2048 0.50 +0.9 2,169,352 - Dsui_2048 0.53 +0.2 2,169,364 + Dsui_2048 0.53 +0.9 2,169,370 - Dsui_2048 0.54 +0.7 2,169,372 - Dsui_2048 0.54 -0.7 2,169,439 - Dsui_2048 0.58 +1.4 2,169,476 + Dsui_2048 0.60 +0.9 2,169,592 - Dsui_2048 0.67 -0.0 2,169,816 - Dsui_2048 0.80 -0.5 2,170,001 + -0.1 2,170,131 + -0.6 2,170,339 - +0.9 2,170,375 - +0.9 2,170,397 - +0.9 2,170,453 + Dsui_2049 0.12 -0.1 2,170,483 - +0.9 2,170,616 + +0.9 2,170,617 + Dsui_2049 0.22 -0.6 2,170,617 + Dsui_2049 0.22 -0.1 2,170,666 - Dsui_2049 0.25 -0.6 2,170,685 - Dsui_2049 0.26 -1.6 2,170,863 + Dsui_2049 0.37 -0.1 2,170,996 + +0.9 2,171,004 - Dsui_2049 0.45 +0.5 2,171,128 - Dsui_2049 0.52 -1.4 2,171,130 + Dsui_2049 0.52 -1.1 2,171,130 + Dsui_2049 0.52 -1.6 2,171,138 - Dsui_2049 0.53 -1.1 2,171,138 - Dsui_2049 0.53 +0.9 2,171,138 - Dsui_2049 0.53 -0.6 2,171,153 - Dsui_2049 0.54 -0.1 2,171,155 - Dsui_2049 0.54 +0.9 2,171,166 + Dsui_2049 0.54 -0.6 2,171,168 - Dsui_2049 0.54 +0.9 2,171,174 - Dsui_2049 0.55 -0.6 2,171,194 - Dsui_2049 0.56 -0.6 2,171,214 - Dsui_2049 0.57 -1.1 2,171,232 - Dsui_2049 0.58 -0.1 2,171,270 - -0.1 2,171,361 - Dsui_2049 0.66 -0.6 2,171,390 + Dsui_2049 0.67 +0.9 2,171,410 - Dsui_2049 0.69 -0.1 2,171,480 + +0.9 2,171,525 - Dsui_2049 0.75 +0.9 2,171,578 - +0.9 2,171,670 + Dsui_2049 0.84 -0.1 2,171,730 - Dsui_2049 0.87 -1.6 2,171,805 + -0.6 2,171,822 + -0.1 2,171,850 + -0.1 2,172,045 - Dsui_2050 0.11 -0.1 2,172,106 + Dsui_2050 0.27 -0.6 2,172,111 + +0.9 2,172,114 - Dsui_2050 0.30 -0.1 2,172,114 - Dsui_2050 0.30 -1.1 2,172,119 - Dsui_2050 0.31 -2.5 2,172,217 - Dsui_2050 0.57 -0.1 2,172,386 - -0.1 2,172,405 - -0.6 2,172,667 + Dsui_2051 0.11 -0.1 2,172,677 + +0.9 2,172,729 - Dsui_2051 0.47 +0.9 2,172,831 + -0.1 2,172,908 + Dsui_2052 0.47 -0.1 2,172,931 - Dsui_2052 0.59 -3.8
Or see this region's nucleotide sequence