Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1988 and Dsui_1989 are separated by 135 nucleotides Dsui_1989 and Dsui_1990 are separated by 73 nucleotides Dsui_1990 and Dsui_1991 overlap by 35 nucleotides Dsui_1991 and Dsui_1992 are separated by 15 nucleotides Dsui_1992 and Dsui_1993 are separated by 94 nucleotides
Dsui_1988: Dsui_1988 - transcriptional regulator, at 2,097,554 to 2,098,528
_1988
Dsui_1989: Dsui_1989 - ribosomal protein S6, at 2,098,664 to 2,099,047
_1989
Dsui_1990: Dsui_1990 - primosomal replication protein N, at 2,099,121 to 2,099,411
_1990
Dsui_1991: Dsui_1991 - ribosomal protein S18, at 2,099,377 to 2,099,655
_1991
Dsui_1992: Dsui_1992 - ribosomal protein L9, at 2,099,671 to 2,100,120
_1992
Dsui_1993: Dsui_1993 - replicative DNA helicase, at 2,100,215 to 2,101,696
_1993
Position (kb)
2099
2100 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2098.522 kb on + strand at 2098.522 kb on + strand at 2098.522 kb on + strand at 2098.522 kb on + strand at 2098.522 kb on + strand at 2098.530 kb on - strand at 2098.530 kb on - strand at 2098.530 kb on - strand at 2099.769 kb on + strand, within Dsui_1992 at 2099.891 kb on + strand, within Dsui_1992 at 2100.163 kb on + strand at 2100.218 kb on - strand at 2100.228 kb on + strand at 2100.228 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 2,098,522 + +0.1 2,098,522 + +0.5 2,098,522 + +0.0 2,098,522 + +0.8 2,098,522 + +1.2 2,098,530 - -0.5 2,098,530 - +1.1 2,098,530 - +1.1 2,099,769 + Dsui_1992 0.22 -4.0 2,099,891 + Dsui_1992 0.49 -1.9 2,100,163 + +1.7 2,100,218 - -2.9 2,100,228 + +1.0 2,100,228 + +0.5
Or see this region's nucleotide sequence