Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1612 and Dsui_1613 are separated by 420 nucleotides Dsui_1613 and Dsui_1614 are separated by 112 nucleotides Dsui_1614 and Dsui_1615 are separated by 41 nucleotides Dsui_1615 and Dsui_1616 are separated by 58 nucleotides
Dsui_1612: Dsui_1612 - hypothetical protein, at 1,695,969 to 1,696,277
_1612
Dsui_1613: Dsui_1613 - cytochrome c553, at 1,696,698 to 1,697,384
_1613
Dsui_1614: Dsui_1614 - hypothetical protein, at 1,697,497 to 1,697,661
_1614
Dsui_1615: Dsui_1615 - hypothetical protein, at 1,697,703 to 1,697,885
_1615
Dsui_1616: Dsui_1616 - nitroreductase, at 1,697,944 to 1,698,534
_1616
Position (kb)
1696
1697
1698 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1695.754 kb on + strand at 1695.772 kb on + strand at 1695.785 kb on - strand at 1695.819 kb on + strand at 1695.827 kb on - strand at 1695.829 kb on - strand at 1695.865 kb on + strand at 1695.879 kb on - strand at 1696.000 kb on + strand, within Dsui_1612 at 1696.005 kb on + strand, within Dsui_1612 at 1696.008 kb on + strand, within Dsui_1612 at 1696.008 kb on + strand, within Dsui_1612 at 1696.016 kb on - strand, within Dsui_1612 at 1696.058 kb on + strand, within Dsui_1612 at 1696.161 kb on + strand, within Dsui_1612 at 1696.164 kb on - strand at 1696.194 kb on + strand, within Dsui_1612 at 1696.202 kb on - strand, within Dsui_1612 at 1696.271 kb on + strand at 1696.271 kb on + strand at 1696.279 kb on - strand at 1696.289 kb on + strand at 1696.307 kb on + strand at 1696.310 kb on - strand at 1696.336 kb on + strand at 1696.386 kb on - strand at 1696.447 kb on + strand at 1696.691 kb on - strand at 1696.746 kb on + strand at 1696.771 kb on + strand, within Dsui_1613 at 1696.908 kb on - strand, within Dsui_1613 at 1696.949 kb on + strand, within Dsui_1613 at 1696.949 kb on + strand at 1697.044 kb on + strand at 1697.044 kb on + strand, within Dsui_1613 at 1697.052 kb on - strand, within Dsui_1613 at 1697.113 kb on - strand, within Dsui_1613 at 1697.122 kb on - strand, within Dsui_1613 at 1697.172 kb on - strand, within Dsui_1613 at 1697.373 kb on - strand at 1697.489 kb on + strand at 1697.609 kb on + strand, within Dsui_1614 at 1697.622 kb on - strand, within Dsui_1614 at 1697.648 kb on + strand at 1697.814 kb on - strand, within Dsui_1615 at 1697.821 kb on - strand, within Dsui_1615 at 1697.834 kb on - strand, within Dsui_1615 at 1697.984 kb on + strand at 1698.260 kb on + strand at 1698.383 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 1,695,754 + +0.5 1,695,772 + +0.5 1,695,785 - -0.5 1,695,819 + -1.8 1,695,827 - -0.5 1,695,829 - -0.5 1,695,865 + -0.5 1,695,879 - -1.5 1,696,000 + Dsui_1612 0.10 -1.1 1,696,005 + Dsui_1612 0.12 +0.5 1,696,008 + Dsui_1612 0.13 -1.5 1,696,008 + Dsui_1612 0.13 -0.5 1,696,016 - Dsui_1612 0.15 -1.1 1,696,058 + Dsui_1612 0.29 +0.5 1,696,161 + Dsui_1612 0.62 +0.5 1,696,164 - +0.5 1,696,194 + Dsui_1612 0.73 +0.5 1,696,202 - Dsui_1612 0.75 -1.1 1,696,271 + +0.5 1,696,271 + +0.5 1,696,279 - -1.1 1,696,289 + -2.8 1,696,307 + -1.5 1,696,310 - -1.5 1,696,336 + -1.1 1,696,386 - +0.5 1,696,447 + +0.5 1,696,691 - -0.5 1,696,746 + +0.5 1,696,771 + Dsui_1613 0.11 -1.1 1,696,908 - Dsui_1613 0.31 +0.4 1,696,949 + Dsui_1613 0.37 +0.5 1,696,949 + +0.5 1,697,044 + -0.5 1,697,044 + Dsui_1613 0.50 -1.1 1,697,052 - Dsui_1613 0.52 +1.0 1,697,113 - Dsui_1613 0.60 -0.1 1,697,122 - Dsui_1613 0.62 +0.8 1,697,172 - Dsui_1613 0.69 -0.4 1,697,373 - -0.7 1,697,489 + -1.8 1,697,609 + Dsui_1614 0.68 +0.5 1,697,622 - Dsui_1614 0.76 +0.5 1,697,648 + +0.5 1,697,814 - Dsui_1615 0.61 +0.5 1,697,821 - Dsui_1615 0.64 -0.5 1,697,834 - Dsui_1615 0.72 -2.1 1,697,984 + -0.5 1,698,260 + -0.5 1,698,383 + +0.5
Or see this region's nucleotide sequence