Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1570 and Dsui_1571 are separated by 99 nucleotides Dsui_1571 and Dsui_1572 are separated by 83 nucleotides Dsui_1572 and Dsui_1573 are separated by 141 nucleotides
Dsui_1570: Dsui_1570 - amino-acid N-acetyltransferase, at 1,643,087 to 1,644,433
_1570
Dsui_1571: Dsui_1571 - Fe-S cluster protector protein, at 1,644,533 to 1,644,805
_1571
Dsui_1572: Dsui_1572 - hypothetical protein, at 1,644,889 to 1,645,296
_1572
Dsui_1573: Dsui_1573 - phosphate transport system regulatory protein PhoU, at 1,645,438 to 1,646,148
_1573
Position (kb)
1644
1645 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1644.412 kb on + strand at 1644.477 kb on + strand at 1644.490 kb on - strand at 1644.775 kb on + strand, within Dsui_1571 at 1644.775 kb on + strand, within Dsui_1571 at 1644.783 kb on - strand at 1644.783 kb on - strand at 1644.789 kb on - strand at 1644.794 kb on + strand at 1644.921 kb on + strand at 1645.013 kb on - strand, within Dsui_1572 at 1645.071 kb on + strand, within Dsui_1572 at 1645.071 kb on + strand, within Dsui_1572 at 1645.129 kb on - strand, within Dsui_1572 at 1645.186 kb on + strand, within Dsui_1572 at 1645.194 kb on - strand at 1645.216 kb on - strand, within Dsui_1572 at 1645.408 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 1,644,412 + -0.2 1,644,477 + +0.5 1,644,490 - -2.3 1,644,775 + Dsui_1571 0.89 +1.9 1,644,775 + Dsui_1571 0.89 +1.6 1,644,783 - +0.5 1,644,783 - -2.1 1,644,789 - +0.5 1,644,794 + +1.9 1,644,921 + -0.8 1,645,013 - Dsui_1572 0.30 -2.3 1,645,071 + Dsui_1572 0.45 -0.8 1,645,071 + Dsui_1572 0.45 +0.5 1,645,129 - Dsui_1572 0.59 -0.8 1,645,186 + Dsui_1572 0.73 +1.7 1,645,194 - +0.5 1,645,216 - Dsui_1572 0.80 -0.5 1,645,408 + -2.8
Or see this region's nucleotide sequence