Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1209 and Dsui_1210 are separated by 80 nucleotides Dsui_1210 and Dsui_1211 are separated by 40 nucleotides Dsui_1211 and Dsui_1212 are separated by 93 nucleotides Dsui_1212 and Dsui_1213 are separated by 113 nucleotides
Dsui_1209: Dsui_1209 - RNA polymerase sigma factor, sigma-70 family, at 1,265,765 to 1,267,735
_1209
Dsui_1210: Dsui_1210 - DNA primase, catalytic core, at 1,267,816 to 1,269,570
_1210
Dsui_1211: Dsui_1211 - hypothetical protein, at 1,269,611 to 1,270,057
_1211
Dsui_1212: Dsui_1212 - ribosomal protein S21, at 1,270,151 to 1,270,363
_1212
Dsui_1213: Dsui_1213 - putative glycoprotease GCP, at 1,270,477 to 1,271,505
_1213
Position (kb)
1267
1268
1269
1270 Strain fitness (log2 ratio)
-1
0
1 at 1269.627 kb on + strand at 1269.634 kb on - strand at 1269.686 kb on - strand, within Dsui_1211 at 1269.686 kb on - strand, within Dsui_1211 at 1269.686 kb on - strand, within Dsui_1211 at 1269.741 kb on + strand, within Dsui_1211 at 1269.749 kb on - strand, within Dsui_1211 at 1270.028 kb on - strand at 1270.060 kb on - strand at 1270.148 kb on - strand at 1270.461 kb on + strand at 1270.469 kb on - strand at 1270.469 kb on - strand at 1270.469 kb on - strand at 1270.469 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 1,269,627 + +0.4 1,269,634 - +0.3 1,269,686 - Dsui_1211 0.17 -0.8 1,269,686 - Dsui_1211 0.17 +0.2 1,269,686 - Dsui_1211 0.17 -0.5 1,269,741 + Dsui_1211 0.29 -0.6 1,269,749 - Dsui_1211 0.31 -0.5 1,270,028 - -1.3 1,270,060 - +0.3 1,270,148 - +0.9 1,270,461 + +0.2 1,270,469 - +0.4 1,270,469 - -0.6 1,270,469 - -0.2 1,270,469 - -0.6
Or see this region's nucleotide sequence