Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1200 and Dsui_1201 are separated by 22 nucleotides Dsui_1201 and Dsui_1202 are separated by 4 nucleotides Dsui_1202 and Dsui_1203 overlap by 23 nucleotides Dsui_1203 and Dsui_1204 are separated by 23 nucleotides
Dsui_1200: Dsui_1200 - glycyl-tRNA synthetase, tetrameric type, beta subunit, at 1,252,378 to 1,254,621
_1200
Dsui_1201: Dsui_1201 - histidinol-phosphate phosphatase family protein, at 1,254,644 to 1,255,192
_1201
Dsui_1202: Dsui_1202 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 1,255,197 to 1,255,940
_1202
Dsui_1203: Dsui_1203 - putative metal-dependent hydrolase, at 1,255,918 to 1,256,694
_1203
Dsui_1204: Dsui_1204 - lactoylglutathione lyase, at 1,256,718 to 1,257,104
_1204
Position (kb)
1255
1256 Strain fitness (log2 ratio)
-2
-1
0
1 at 1256.077 kb on - strand, within Dsui_1203 at 1256.089 kb on - strand, within Dsui_1203 at 1256.121 kb on - strand, within Dsui_1203 at 1256.166 kb on - strand, within Dsui_1203 at 1256.171 kb on - strand, within Dsui_1203 at 1256.181 kb on + strand, within Dsui_1203 at 1256.384 kb on + strand, within Dsui_1203 at 1256.392 kb on - strand, within Dsui_1203 at 1256.430 kb on - strand, within Dsui_1203 at 1256.430 kb on - strand, within Dsui_1203 at 1256.646 kb on + strand at 1256.670 kb on + strand at 1256.759 kb on - strand, within Dsui_1204 at 1256.769 kb on + strand, within Dsui_1204 at 1256.769 kb on - strand, within Dsui_1204 at 1256.892 kb on - strand, within Dsui_1204 at 1256.911 kb on + strand, within Dsui_1204 at 1256.929 kb on + strand, within Dsui_1204
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 1,256,077 - Dsui_1203 0.20 -1.6 1,256,089 - Dsui_1203 0.22 -1.9 1,256,121 - Dsui_1203 0.26 +0.4 1,256,166 - Dsui_1203 0.32 -1.2 1,256,171 - Dsui_1203 0.33 +0.4 1,256,181 + Dsui_1203 0.34 -0.1 1,256,384 + Dsui_1203 0.60 -0.5 1,256,392 - Dsui_1203 0.61 +0.4 1,256,430 - Dsui_1203 0.66 +0.4 1,256,430 - Dsui_1203 0.66 -2.7 1,256,646 + +0.4 1,256,670 + +0.7 1,256,759 - Dsui_1204 0.11 +0.4 1,256,769 + Dsui_1204 0.13 +0.1 1,256,769 - Dsui_1204 0.13 +0.4 1,256,892 - Dsui_1204 0.45 -0.6 1,256,911 + Dsui_1204 0.50 -0.3 1,256,929 + Dsui_1204 0.55 -0.2
Or see this region's nucleotide sequence