Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1175 and Dsui_1176 are separated by 283 nucleotides Dsui_1176 and Dsui_1177 are separated by 109 nucleotides Dsui_1177 and Dsui_1178 are separated by 102 nucleotides Dsui_1178 and Dsui_1179 overlap by 4 nucleotides
Dsui_1175: Dsui_1175 - cytochrome c-type biogenesis protein CcmI, at 1,229,533 to 1,230,396
_1175
Dsui_1176: Dsui_1176 - periplasmic nitrate/nitrite reductase c-type cytochrome, NapC/NirT family, at 1,230,680 to 1,231,270
_1176
Dsui_1177: Dsui_1177 - Ethylbenzene dehydrogenase, at 1,231,380 to 1,232,249
_1177
Dsui_1178: Dsui_1178 - ferredoxin-type protein NapF, at 1,232,352 to 1,232,852
_1178
Dsui_1179: Dsui_1179 - ribosome-associated heat shock protein implicated in recycling of 50S subunit, at 1,232,849 to 1,233,268
_1179
Position (kb)
1231
1232
1233 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1230.466 kb on + strand at 1230.525 kb on - strand at 1230.560 kb on + strand at 1230.568 kb on - strand at 1230.605 kb on - strand at 1230.648 kb on - strand at 1230.721 kb on - strand at 1230.722 kb on - strand at 1230.722 kb on - strand at 1230.754 kb on + strand, within Dsui_1176 at 1230.819 kb on + strand, within Dsui_1176 at 1230.825 kb on + strand, within Dsui_1176 at 1230.825 kb on + strand, within Dsui_1176 at 1230.875 kb on + strand, within Dsui_1176 at 1230.964 kb on - strand, within Dsui_1176 at 1230.995 kb on - strand, within Dsui_1176 at 1231.060 kb on + strand, within Dsui_1176 at 1231.073 kb on - strand, within Dsui_1176 at 1231.142 kb on + strand, within Dsui_1176 at 1231.203 kb on + strand, within Dsui_1176 at 1231.221 kb on + strand at 1231.227 kb on - strand at 1231.238 kb on + strand at 1231.238 kb on + strand at 1231.239 kb on - strand at 1231.246 kb on - strand at 1231.246 kb on - strand at 1231.246 kb on - strand at 1231.246 kb on - strand at 1231.292 kb on + strand at 1231.311 kb on + strand at 1231.380 kb on - strand at 1231.380 kb on - strand at 1231.408 kb on - strand at 1231.428 kb on - strand at 1231.470 kb on - strand, within Dsui_1177 at 1231.516 kb on - strand, within Dsui_1177 at 1231.544 kb on + strand, within Dsui_1177 at 1231.568 kb on + strand, within Dsui_1177 at 1231.569 kb on + strand, within Dsui_1177 at 1231.569 kb on + strand, within Dsui_1177 at 1231.570 kb on - strand, within Dsui_1177 at 1231.588 kb on + strand, within Dsui_1177 at 1231.680 kb on - strand, within Dsui_1177 at 1231.788 kb on - strand, within Dsui_1177 at 1231.858 kb on - strand, within Dsui_1177 at 1231.874 kb on + strand, within Dsui_1177 at 1231.958 kb on - strand, within Dsui_1177 at 1232.060 kb on + strand, within Dsui_1177 at 1232.068 kb on - strand, within Dsui_1177 at 1232.088 kb on - strand at 1232.197 kb on + strand at 1232.224 kb on - strand at 1232.306 kb on - strand at 1232.357 kb on - strand at 1232.357 kb on - strand at 1232.441 kb on + strand, within Dsui_1178 at 1232.633 kb on + strand, within Dsui_1178 at 1232.658 kb on + strand at 1232.914 kb on - strand, within Dsui_1179 at 1233.052 kb on + strand, within Dsui_1179 at 1233.105 kb on - strand, within Dsui_1179 at 1233.242 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 1,230,466 + -0.2 1,230,525 - -1.6 1,230,560 + -0.4 1,230,568 - -1.2 1,230,605 - +0.4 1,230,648 - +0.4 1,230,721 - -0.6 1,230,722 - +0.4 1,230,722 - +0.4 1,230,754 + Dsui_1176 0.13 +0.4 1,230,819 + Dsui_1176 0.24 +0.1 1,230,825 + Dsui_1176 0.25 +0.6 1,230,825 + Dsui_1176 0.25 +0.3 1,230,875 + Dsui_1176 0.33 -0.1 1,230,964 - Dsui_1176 0.48 -1.6 1,230,995 - Dsui_1176 0.53 -1.6 1,231,060 + Dsui_1176 0.64 +0.0 1,231,073 - Dsui_1176 0.66 -0.6 1,231,142 + Dsui_1176 0.78 -0.3 1,231,203 + Dsui_1176 0.88 -0.0 1,231,221 + -0.1 1,231,227 - +0.4 1,231,238 + -0.4 1,231,238 + -0.3 1,231,239 - +0.4 1,231,246 - +0.4 1,231,246 - +0.4 1,231,246 - +0.8 1,231,246 - +0.4 1,231,292 + +0.1 1,231,311 + +0.0 1,231,380 - -0.1 1,231,380 - -0.2 1,231,408 - -3.3 1,231,428 - -1.2 1,231,470 - Dsui_1177 0.10 -1.6 1,231,516 - Dsui_1177 0.16 -0.6 1,231,544 + Dsui_1177 0.19 +0.2 1,231,568 + Dsui_1177 0.22 +0.0 1,231,569 + Dsui_1177 0.22 +0.5 1,231,569 + Dsui_1177 0.22 +0.2 1,231,570 - Dsui_1177 0.22 -0.6 1,231,588 + Dsui_1177 0.24 +0.1 1,231,680 - Dsui_1177 0.34 +1.4 1,231,788 - Dsui_1177 0.47 +1.2 1,231,858 - Dsui_1177 0.55 -1.9 1,231,874 + Dsui_1177 0.57 -0.9 1,231,958 - Dsui_1177 0.66 -2.4 1,232,060 + Dsui_1177 0.78 +1.3 1,232,068 - Dsui_1177 0.79 -1.6 1,232,088 - -0.6 1,232,197 + -0.2 1,232,224 - +0.4 1,232,306 - +0.4 1,232,357 - -1.6 1,232,357 - +1.4 1,232,441 + Dsui_1178 0.18 -2.2 1,232,633 + Dsui_1178 0.56 -0.6 1,232,658 + -0.6 1,232,914 - Dsui_1179 0.15 +0.2 1,233,052 + Dsui_1179 0.48 -2.2 1,233,105 - Dsui_1179 0.61 +0.7 1,233,242 + -1.2
Or see this region's nucleotide sequence