Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1147 and Dsui_1148 are separated by 10 nucleotides Dsui_1148 and Dsui_1149 are separated by 178 nucleotides Dsui_1149 and Dsui_1150 are separated by 24 nucleotides Dsui_1150 and Dsui_1151 are separated by 104 nucleotides
Dsui_1147: Dsui_1147 - PAS domain S-box, at 1,202,101 to 1,203,474
_1147
Dsui_1148: Dsui_1148 - putative transcriptional regulator with CopG/Arc/MetJ DNA-binding domain and metal-binding domain, at 1,203,485 to 1,203,973
_1148
Dsui_1149: Dsui_1149 - Zn-dependent protease with chaperone function, at 1,204,152 to 1,205,033
_1149
Dsui_1150: Dsui_1150 - Sigma 54 modulation protein / S30EA ribosomal protein, at 1,205,058 to 1,205,441
_1150
Dsui_1151: Dsui_1151 - transcriptional regulator, at 1,205,546 to 1,206,466
_1151
Position (kb)
1204
1205
1206 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1203.203 kb on + strand, within Dsui_1147 at 1203.295 kb on + strand at 1203.302 kb on - strand, within Dsui_1147 at 1203.313 kb on - strand, within Dsui_1147 at 1203.315 kb on + strand, within Dsui_1147 at 1203.346 kb on + strand at 1203.630 kb on + strand, within Dsui_1148 at 1203.650 kb on - strand, within Dsui_1148 at 1203.846 kb on - strand, within Dsui_1148 at 1203.890 kb on - strand, within Dsui_1148 at 1204.109 kb on + strand at 1204.427 kb on + strand, within Dsui_1149 at 1204.435 kb on - strand, within Dsui_1149 at 1204.447 kb on - strand, within Dsui_1149 at 1204.561 kb on - strand, within Dsui_1149 at 1204.605 kb on + strand, within Dsui_1149 at 1204.622 kb on + strand, within Dsui_1149 at 1204.820 kb on + strand, within Dsui_1149 at 1204.859 kb on + strand at 1204.976 kb on + strand at 1204.977 kb on + strand at 1205.001 kb on + strand at 1205.035 kb on + strand at 1205.269 kb on + strand, within Dsui_1150 at 1205.479 kb on + strand at 1205.479 kb on + strand at 1205.487 kb on - strand at 1205.582 kb on - strand at 1205.660 kb on + strand, within Dsui_1151 at 1205.680 kb on + strand, within Dsui_1151 at 1205.688 kb on + strand, within Dsui_1151 at 1205.707 kb on - strand, within Dsui_1151 at 1205.770 kb on + strand, within Dsui_1151 at 1205.770 kb on + strand, within Dsui_1151 at 1205.778 kb on - strand, within Dsui_1151 at 1205.824 kb on + strand, within Dsui_1151 at 1205.844 kb on + strand, within Dsui_1151 at 1205.873 kb on - strand at 1205.886 kb on - strand, within Dsui_1151 at 1205.931 kb on - strand, within Dsui_1151 at 1205.992 kb on - strand, within Dsui_1151 at 1205.992 kb on - strand, within Dsui_1151 at 1206.002 kb on - strand, within Dsui_1151
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 1,203,203 + Dsui_1147 0.80 -0.5 1,203,295 + +0.5 1,203,302 - Dsui_1147 0.87 -0.6 1,203,313 - Dsui_1147 0.88 +0.2 1,203,315 + Dsui_1147 0.88 +0.5 1,203,346 + -1.1 1,203,630 + Dsui_1148 0.30 -2.5 1,203,650 - Dsui_1148 0.34 +0.4 1,203,846 - Dsui_1148 0.74 +0.2 1,203,890 - Dsui_1148 0.83 -0.8 1,204,109 + -1.1 1,204,427 + Dsui_1149 0.31 -0.5 1,204,435 - Dsui_1149 0.32 -1.1 1,204,447 - Dsui_1149 0.33 +0.5 1,204,561 - Dsui_1149 0.46 +0.9 1,204,605 + Dsui_1149 0.51 -0.5 1,204,622 + Dsui_1149 0.53 -1.5 1,204,820 + Dsui_1149 0.76 -3.8 1,204,859 + +0.5 1,204,976 + -2.3 1,204,977 + -1.1 1,205,001 + -0.5 1,205,035 + -0.5 1,205,269 + Dsui_1150 0.55 +0.5 1,205,479 + +0.5 1,205,479 + -0.7 1,205,487 - +0.5 1,205,582 - -2.5 1,205,660 + Dsui_1151 0.12 -0.9 1,205,680 + Dsui_1151 0.15 -1.1 1,205,688 + Dsui_1151 0.15 -0.6 1,205,707 - Dsui_1151 0.17 +0.5 1,205,770 + Dsui_1151 0.24 -0.5 1,205,770 + Dsui_1151 0.24 -0.3 1,205,778 - Dsui_1151 0.25 -0.5 1,205,824 + Dsui_1151 0.30 -0.4 1,205,844 + Dsui_1151 0.32 -0.5 1,205,873 - -0.5 1,205,886 - Dsui_1151 0.37 -0.5 1,205,931 - Dsui_1151 0.42 -1.9 1,205,992 - Dsui_1151 0.48 -2.9 1,205,992 - Dsui_1151 0.48 -1.9 1,206,002 - Dsui_1151 0.50 -0.5
Or see this region's nucleotide sequence