Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1051 and Dsui_1052 are separated by 60 nucleotides Dsui_1052 and Dsui_1053 overlap by 4 nucleotides Dsui_1053 and Dsui_1054 are separated by 108 nucleotides
Dsui_1051: Dsui_1051 - nitrogen regulation protein NR(I), at 1,084,183 to 1,085,619
_1051
Dsui_1052: Dsui_1052 - birA, biotin-(acetyl-CoA-carboxylase) ligase, at 1,085,680 to 1,086,555
_1052
Dsui_1053: Dsui_1053 - pantothenate kinase, type III, at 1,086,552 to 1,087,310
_1053
Dsui_1054: Dsui_1054 - pyridoxal-phosphate dependent TrpB-like enzyme, at 1,087,419 to 1,088,783
_1054
Position (kb)
1085
1086
1087 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1084.727 kb on + strand, within Dsui_1051 at 1084.735 kb on - strand, within Dsui_1051 at 1084.738 kb on + strand, within Dsui_1051 at 1084.853 kb on + strand, within Dsui_1051 at 1084.863 kb on + strand, within Dsui_1051 at 1084.871 kb on - strand, within Dsui_1051 at 1085.073 kb on + strand, within Dsui_1051 at 1085.162 kb on + strand, within Dsui_1051 at 1085.169 kb on - strand, within Dsui_1051 at 1085.523 kb on + strand at 1085.575 kb on - strand at 1085.585 kb on - strand at 1085.593 kb on - strand at 1085.664 kb on + strand at 1085.671 kb on + strand at 1085.672 kb on - strand at 1085.674 kb on - strand at 1085.737 kb on - strand at 1085.748 kb on + strand at 1085.789 kb on - strand at 1086.557 kb on - strand at 1087.280 kb on - strand at 1087.388 kb on + strand at 1087.388 kb on + strand at 1087.388 kb on + strand at 1087.396 kb on - strand at 1087.429 kb on + strand at 1087.429 kb on + strand at 1087.429 kb on + strand at 1087.429 kb on + strand at 1087.463 kb on - strand at 1087.487 kb on + strand at 1087.495 kb on - strand at 1087.495 kb on - strand at 1087.547 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 1,084,727 + Dsui_1051 0.38 +0.8 1,084,735 - Dsui_1051 0.38 -0.3 1,084,738 + Dsui_1051 0.39 +0.1 1,084,853 + Dsui_1051 0.47 -1.8 1,084,863 + Dsui_1051 0.47 -1.8 1,084,871 - Dsui_1051 0.48 +0.3 1,085,073 + Dsui_1051 0.62 +1.2 1,085,162 + Dsui_1051 0.68 -1.0 1,085,169 - Dsui_1051 0.69 +0.6 1,085,523 + +0.6 1,085,575 - -1.4 1,085,585 - -0.4 1,085,593 - -0.4 1,085,664 + -1.4 1,085,671 + -0.2 1,085,672 - +0.6 1,085,674 - -0.4 1,085,737 - +0.6 1,085,748 + +0.5 1,085,789 - +0.6 1,086,557 - +0.6 1,087,280 - -1.8 1,087,388 + +0.3 1,087,388 + +1.2 1,087,388 + +0.9 1,087,396 - -0.4 1,087,429 + +2.7 1,087,429 + -2.4 1,087,429 + +1.1 1,087,429 + +2.4 1,087,463 - -1.0 1,087,487 + +0.5 1,087,495 - +0.6 1,087,495 - +0.6 1,087,547 + +0.6
Or see this region's nucleotide sequence