Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1003 and Dsui_1004 are separated by 113 nucleotides Dsui_1004 and Dsui_1005 are separated by 88 nucleotides Dsui_1005 and Dsui_1006 are separated by 40 nucleotides
Dsui_1003: Dsui_1003 - phosphoribosylglycinamide formyltransferase 2, at 1,031,262 to 1,032,494
_1003
Dsui_1004: Dsui_1004 - conserved protein of DIM6/NTAB family, at 1,032,608 to 1,033,159
_1004
Dsui_1005: Dsui_1005 - tRNA-Gly, at 1,033,248 to 1,033,321
_1005
Dsui_1006: Dsui_1006 - transcriptional regulator, at 1,033,362 to 1,034,282
_1006
Position (kb)
1032
1033
1034 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1031.647 kb on + strand at 1031.725 kb on - strand, within Dsui_1003 at 1031.748 kb on + strand, within Dsui_1003 at 1031.783 kb on - strand, within Dsui_1003 at 1031.828 kb on + strand at 1031.828 kb on + strand, within Dsui_1003 at 1031.988 kb on - strand, within Dsui_1003 at 1032.031 kb on + strand, within Dsui_1003 at 1032.049 kb on + strand, within Dsui_1003 at 1032.229 kb on - strand, within Dsui_1003 at 1032.384 kb on + strand at 1032.394 kb on - strand at 1032.630 kb on + strand at 1032.710 kb on + strand, within Dsui_1004 at 1032.849 kb on + strand, within Dsui_1004 at 1032.884 kb on - strand at 1032.928 kb on - strand at 1033.006 kb on - strand at 1033.174 kb on + strand at 1033.257 kb on + strand, within Dsui_1005 at 1033.269 kb on + strand, within Dsui_1005 at 1033.277 kb on - strand at 1033.284 kb on + strand, within Dsui_1005 at 1033.349 kb on - strand at 1033.403 kb on - strand at 1033.413 kb on - strand at 1033.712 kb on - strand, within Dsui_1006 at 1033.737 kb on - strand, within Dsui_1006 at 1033.758 kb on - strand, within Dsui_1006 at 1033.845 kb on + strand, within Dsui_1006 at 1033.884 kb on + strand, within Dsui_1006 at 1033.908 kb on - strand, within Dsui_1006 at 1033.985 kb on + strand, within Dsui_1006 at 1033.990 kb on + strand at 1034.149 kb on - strand, within Dsui_1006 at 1034.159 kb on + strand, within Dsui_1006
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 1,031,647 + +0.6 1,031,725 - Dsui_1003 0.38 +0.6 1,031,748 + Dsui_1003 0.39 -1.0 1,031,783 - Dsui_1003 0.42 +1.7 1,031,828 + -0.4 1,031,828 + Dsui_1003 0.46 +0.6 1,031,988 - Dsui_1003 0.59 +1.8 1,032,031 + Dsui_1003 0.62 +0.6 1,032,049 + Dsui_1003 0.64 +0.6 1,032,229 - Dsui_1003 0.78 +1.0 1,032,384 + +0.6 1,032,394 - +1.0 1,032,630 + +3.5 1,032,710 + Dsui_1004 0.18 +2.5 1,032,849 + Dsui_1004 0.44 +3.6 1,032,884 - -1.0 1,032,928 - +0.6 1,033,006 - +0.6 1,033,174 + -0.1 1,033,257 + Dsui_1005 0.12 +0.3 1,033,269 + Dsui_1005 0.28 -0.3 1,033,277 - +0.6 1,033,284 + Dsui_1005 0.49 +0.4 1,033,349 - -2.0 1,033,403 - +1.0 1,033,413 - +1.2 1,033,712 - Dsui_1006 0.38 +0.1 1,033,737 - Dsui_1006 0.41 +0.1 1,033,758 - Dsui_1006 0.43 +0.7 1,033,845 + Dsui_1006 0.52 -1.4 1,033,884 + Dsui_1006 0.57 -0.2 1,033,908 - Dsui_1006 0.59 +0.0 1,033,985 + Dsui_1006 0.68 -1.7 1,033,990 + +0.6 1,034,149 - Dsui_1006 0.85 -0.1 1,034,159 + Dsui_1006 0.87 +0.6
Or see this region's nucleotide sequence