Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0990 and Dsui_0991 overlap by 14 nucleotides Dsui_0991 and Dsui_0992 overlap by 20 nucleotides Dsui_0992 and Dsui_0993 overlap by 8 nucleotides Dsui_0993 and Dsui_0994 overlap by 1 nucleotides
Dsui_0990: Dsui_0990 - putative P-loop-containing kinase, at 1,017,863 to 1,018,735
_0990
Dsui_0991: Dsui_0991 - hypothetical protein, at 1,018,722 to 1,019,150
_0991
Dsui_0992: Dsui_0992 - putative membrane protein, at 1,019,131 to 1,019,706
_0992
Dsui_0993: Dsui_0993 - polyprenyl p-hydroxybenzoate/phenylacrylic acid decarboxylase, at 1,019,699 to 1,020,316
_0993
Dsui_0994: Dsui_0994 - peptidase S16, lon domain protein, at 1,020,316 to 1,020,957
_0994
Position (kb)
1019
1020 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1018.374 kb on + strand, within Dsui_0990 at 1018.764 kb on + strand at 1018.824 kb on - strand at 1018.935 kb on - strand at 1019.036 kb on - strand, within Dsui_0991 at 1019.279 kb on + strand, within Dsui_0992 at 1019.433 kb on + strand, within Dsui_0992 at 1019.488 kb on - strand at 1019.701 kb on + strand at 1019.709 kb on - strand at 1019.709 kb on - strand at 1019.709 kb on - strand at 1019.709 kb on - strand at 1020.458 kb on - strand, within Dsui_0994 at 1020.463 kb on - strand at 1020.500 kb on - strand, within Dsui_0994 at 1020.514 kb on + strand, within Dsui_0994
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 1,018,374 + Dsui_0990 0.59 +0.7 1,018,764 + +0.6 1,018,824 - +0.6 1,018,935 - +0.6 1,019,036 - Dsui_0991 0.73 -0.4 1,019,279 + Dsui_0992 0.26 +1.6 1,019,433 + Dsui_0992 0.52 +2.2 1,019,488 - +0.6 1,019,701 + +0.1 1,019,709 - -0.4 1,019,709 - -0.4 1,019,709 - -0.4 1,019,709 - -1.7 1,020,458 - Dsui_0994 0.22 +0.6 1,020,463 - +0.6 1,020,500 - Dsui_0994 0.29 -2.0 1,020,514 + Dsui_0994 0.31 +0.9
Or see this region's nucleotide sequence