Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0930 and Dsui_0931 are separated by 168 nucleotides Dsui_0931 and Dsui_0932 are separated by 15 nucleotides Dsui_0932 and Dsui_0933 are separated by 196 nucleotides
Dsui_0930: Dsui_0930 - transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains, at 958,448 to 959,797
_0930
Dsui_0931: Dsui_0931 - thiol-disulfide isomerase-like thioredoxin, at 959,966 to 960,472
_0931
Dsui_0932: Dsui_0932 - hypothetical protein, at 960,488 to 961,192
_0932
Dsui_0933: Dsui_0933 - DNA/RNA helicase, superfamily I, at 961,389 to 963,398
_0933
Position (kb) 959
960
961 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 959.064 kb on - strand, within Dsui_0930 at 959.064 kb on - strand, within Dsui_0930 at 959.185 kb on + strand, within Dsui_0930 at 959.246 kb on - strand, within Dsui_0930 at 959.502 kb on + strand, within Dsui_0930 at 959.533 kb on + strand, within Dsui_0930 at 959.537 kb on + strand, within Dsui_0930 at 959.568 kb on + strand, within Dsui_0930 at 959.620 kb on - strand, within Dsui_0930 at 959.695 kb on - strand at 959.700 kb on - strand at 959.747 kb on - strand at 959.747 kb on - strand at 959.747 kb on - strand at 959.784 kb on - strand at 959.819 kb on + strand at 959.835 kb on + strand at 959.858 kb on + strand at 959.858 kb on + strand at 959.866 kb on - strand at 959.868 kb on + strand at 959.944 kb on - strand at 959.950 kb on + strand at 959.950 kb on + strand at 959.972 kb on - strand at 960.035 kb on + strand, within Dsui_0931 at 960.090 kb on - strand, within Dsui_0931 at 960.090 kb on - strand, within Dsui_0931 at 960.100 kb on - strand, within Dsui_0931 at 960.124 kb on + strand, within Dsui_0931 at 960.169 kb on - strand, within Dsui_0931 at 960.196 kb on - strand, within Dsui_0931 at 960.213 kb on - strand, within Dsui_0931 at 960.273 kb on - strand, within Dsui_0931 at 960.349 kb on - strand, within Dsui_0931 at 960.445 kb on + strand at 960.445 kb on + strand at 960.448 kb on - strand at 960.453 kb on - strand at 960.453 kb on - strand at 960.575 kb on + strand, within Dsui_0932 at 960.597 kb on - strand, within Dsui_0932 at 960.663 kb on - strand, within Dsui_0932 at 960.711 kb on + strand, within Dsui_0932 at 960.870 kb on - strand, within Dsui_0932 at 961.001 kb on - strand, within Dsui_0932 at 961.032 kb on - strand at 961.087 kb on - strand, within Dsui_0932 at 961.089 kb on + strand, within Dsui_0932 at 961.089 kb on + strand, within Dsui_0932 at 961.089 kb on + strand, within Dsui_0932 at 961.097 kb on - strand, within Dsui_0932 at 961.097 kb on - strand, within Dsui_0932 at 961.122 kb on - strand at 961.177 kb on - strand at 961.346 kb on - strand at 961.404 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 959,064 - Dsui_0930 0.46 -1.0 959,064 - Dsui_0930 0.46 +0.5 959,185 + Dsui_0930 0.55 +0.3 959,246 - Dsui_0930 0.59 -1.0 959,502 + Dsui_0930 0.78 +0.2 959,533 + Dsui_0930 0.80 +0.0 959,537 + Dsui_0930 0.81 -0.4 959,568 + Dsui_0930 0.83 +0.1 959,620 - Dsui_0930 0.87 -0.5 959,695 - +0.5 959,700 - -1.0 959,747 - -2.0 959,747 - +0.5 959,747 - -1.3 959,784 - -0.5 959,819 + +0.0 959,835 + +0.7 959,858 + -1.2 959,858 + +0.5 959,866 - +0.5 959,868 + +0.8 959,944 - -2.6 959,950 + -0.5 959,950 + +3.1 959,972 - -0.0 960,035 + Dsui_0931 0.14 -2.0 960,090 - Dsui_0931 0.24 -0.4 960,090 - Dsui_0931 0.24 +0.5 960,100 - Dsui_0931 0.26 +1.8 960,124 + Dsui_0931 0.31 -0.5 960,169 - Dsui_0931 0.40 +0.3 960,196 - Dsui_0931 0.45 -0.7 960,213 - Dsui_0931 0.49 +0.1 960,273 - Dsui_0931 0.61 +0.7 960,349 - Dsui_0931 0.76 +1.5 960,445 + +0.5 960,445 + +0.2 960,448 - +1.0 960,453 - +0.2 960,453 - +0.4 960,575 + Dsui_0932 0.12 -2.2 960,597 - Dsui_0932 0.15 -1.8 960,663 - Dsui_0932 0.25 -0.5 960,711 + Dsui_0932 0.32 -0.3 960,870 - Dsui_0932 0.54 +0.5 961,001 - Dsui_0932 0.73 +0.5 961,032 - -1.0 961,087 - Dsui_0932 0.85 +0.5 961,089 + Dsui_0932 0.85 +0.2 961,089 + Dsui_0932 0.85 -2.2 961,089 + Dsui_0932 0.85 -0.4 961,097 - Dsui_0932 0.86 -1.8 961,097 - Dsui_0932 0.86 +0.5 961,122 - +0.5 961,177 - +0.5 961,346 - -1.3 961,404 + +0.7
Or see this region's nucleotide sequence