Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0686 and Dsui_0687 overlap by 17 nucleotides Dsui_0687 and Dsui_0688 are separated by 95 nucleotides Dsui_0688 and Dsui_0689 overlap by 1 nucleotides Dsui_0689 and Dsui_0690 are separated by 72 nucleotides Dsui_0690 and Dsui_0691 are separated by 160 nucleotides Dsui_0691 and Dsui_0692 are separated by 17 nucleotides
Dsui_0686: Dsui_0686 - tRNA nucleotidyltransferase/poly(A) polymerase, at 684,017 to 685,237
_0686
Dsui_0687: Dsui_0687 - Protein of unknown function (DUF2905), at 685,221 to 685,412
_0687
Dsui_0688: Dsui_0688 - glutathione S-transferase, at 685,508 to 686,125
_0688
Dsui_0689: Dsui_0689 - lysophospholipase, at 686,125 to 686,937
_0689
Dsui_0690: Dsui_0690 - hypothetical protein, at 687,010 to 687,204
_0690
Dsui_0691: Dsui_0691 - hypothetical protein, at 687,365 to 687,562
_0691
Dsui_0692: Dsui_0692 - hypothetical protein, at 687,580 to 688,434
_0692
Position (kb) 686
687 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 685.268 kb on - strand, within Dsui_0687 at 685.274 kb on - strand, within Dsui_0687 at 685.318 kb on - strand, within Dsui_0687 at 685.379 kb on + strand, within Dsui_0687 at 685.387 kb on - strand, within Dsui_0687 at 685.422 kb on - strand at 685.493 kb on + strand at 685.501 kb on - strand at 685.513 kb on - strand at 685.514 kb on + strand at 685.525 kb on - strand at 685.574 kb on + strand, within Dsui_0688 at 685.579 kb on + strand, within Dsui_0688 at 685.579 kb on + strand, within Dsui_0688 at 685.579 kb on + strand, within Dsui_0688 at 685.579 kb on + strand, within Dsui_0688 at 685.587 kb on - strand at 685.587 kb on - strand, within Dsui_0688 at 685.587 kb on - strand, within Dsui_0688 at 685.587 kb on - strand, within Dsui_0688 at 685.587 kb on - strand, within Dsui_0688 at 685.587 kb on - strand at 685.616 kb on - strand at 685.616 kb on - strand, within Dsui_0688 at 685.641 kb on - strand, within Dsui_0688 at 685.701 kb on + strand, within Dsui_0688 at 685.776 kb on + strand, within Dsui_0688 at 685.877 kb on - strand, within Dsui_0688 at 685.921 kb on + strand, within Dsui_0688 at 685.921 kb on + strand, within Dsui_0688 at 685.940 kb on + strand, within Dsui_0688 at 685.952 kb on + strand, within Dsui_0688 at 685.952 kb on + strand, within Dsui_0688 at 686.001 kb on + strand, within Dsui_0688 at 686.106 kb on + strand at 686.114 kb on - strand at 686.114 kb on - strand at 686.190 kb on + strand at 686.329 kb on + strand at 686.329 kb on + strand, within Dsui_0689 at 686.435 kb on - strand, within Dsui_0689 at 686.446 kb on + strand, within Dsui_0689 at 686.485 kb on + strand, within Dsui_0689 at 686.559 kb on + strand, within Dsui_0689 at 686.588 kb on - strand, within Dsui_0689 at 686.797 kb on + strand, within Dsui_0689 at 686.905 kb on + strand at 686.925 kb on - strand at 686.998 kb on + strand at 687.016 kb on - strand at 687.028 kb on - strand at 687.051 kb on + strand, within Dsui_0690 at 687.059 kb on - strand, within Dsui_0690 at 687.069 kb on - strand, within Dsui_0690 at 687.095 kb on + strand, within Dsui_0690 at 687.110 kb on + strand, within Dsui_0690 at 687.129 kb on - strand, within Dsui_0690 at 687.144 kb on + strand at 687.159 kb on - strand, within Dsui_0690 at 687.214 kb on - strand at 687.288 kb on + strand at 687.294 kb on + strand at 687.311 kb on + strand at 687.311 kb on + strand at 687.311 kb on + strand at 687.311 kb on + strand at 687.311 kb on + strand at 687.319 kb on - strand at 687.319 kb on - strand at 687.342 kb on + strand at 687.348 kb on - strand at 687.386 kb on + strand, within Dsui_0691 at 687.466 kb on + strand, within Dsui_0691 at 687.539 kb on - strand, within Dsui_0691 at 687.556 kb on + strand at 687.581 kb on - strand at 687.647 kb on + strand at 687.655 kb on - strand at 687.716 kb on - strand, within Dsui_0692 at 687.748 kb on - strand at 687.756 kb on + strand, within Dsui_0692 at 687.786 kb on + strand, within Dsui_0692 at 687.856 kb on - strand, within Dsui_0692 at 687.873 kb on - strand, within Dsui_0692
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 685,268 - Dsui_0687 0.24 -0.5 685,274 - Dsui_0687 0.28 +0.2 685,318 - Dsui_0687 0.51 +0.7 685,379 + Dsui_0687 0.82 -0.9 685,387 - Dsui_0687 0.86 +1.2 685,422 - +0.1 685,493 + -1.3 685,501 - -1.2 685,513 - -1.2 685,514 + -3.2 685,525 - -1.6 685,574 + Dsui_0688 0.11 -3.8 685,579 + Dsui_0688 0.11 +2.5 685,579 + Dsui_0688 0.11 +0.8 685,579 + Dsui_0688 0.11 +0.8 685,579 + Dsui_0688 0.11 -2.8 685,587 - +0.4 685,587 - Dsui_0688 0.13 -2.2 685,587 - Dsui_0688 0.13 -0.6 685,587 - Dsui_0688 0.13 -2.0 685,587 - Dsui_0688 0.13 -2.0 685,587 - -0.6 685,616 - +0.4 685,616 - Dsui_0688 0.17 -2.0 685,641 - Dsui_0688 0.22 -2.2 685,701 + Dsui_0688 0.31 -0.4 685,776 + Dsui_0688 0.43 -1.1 685,877 - Dsui_0688 0.60 +0.4 685,921 + Dsui_0688 0.67 -0.8 685,921 + Dsui_0688 0.67 +0.2 685,940 + Dsui_0688 0.70 -0.9 685,952 + Dsui_0688 0.72 -0.3 685,952 + Dsui_0688 0.72 -1.9 686,001 + Dsui_0688 0.80 -0.4 686,106 + -2.2 686,114 - -1.6 686,114 - -1.6 686,190 + +0.1 686,329 + -0.6 686,329 + Dsui_0689 0.25 -1.5 686,435 - Dsui_0689 0.38 -2.0 686,446 + Dsui_0689 0.39 -1.5 686,485 + Dsui_0689 0.44 -0.3 686,559 + Dsui_0689 0.53 +0.1 686,588 - Dsui_0689 0.57 +0.4 686,797 + Dsui_0689 0.83 -1.2 686,905 + -0.9 686,925 - -0.7 686,998 + -0.4 687,016 - +0.3 687,028 - -0.6 687,051 + Dsui_0690 0.21 -0.5 687,059 - Dsui_0690 0.25 -0.3 687,069 - Dsui_0690 0.30 -0.1 687,095 + Dsui_0690 0.44 -1.1 687,110 + Dsui_0690 0.51 +0.3 687,129 - Dsui_0690 0.61 -0.2 687,144 + -0.7 687,159 - Dsui_0690 0.76 -0.6 687,214 - -0.3 687,288 + -1.2 687,294 + +1.7 687,311 + -1.7 687,311 + -0.2 687,311 + -0.7 687,311 + -0.7 687,311 + -1.2 687,319 - -2.0 687,319 - -2.2 687,342 + +0.3 687,348 - +0.3 687,386 + Dsui_0691 0.11 -1.2 687,466 + Dsui_0691 0.51 +0.3 687,539 - Dsui_0691 0.88 -2.7 687,556 + +0.3 687,581 - -2.5 687,647 + +0.3 687,655 - -1.2 687,716 - Dsui_0692 0.16 +1.3 687,748 - +0.3 687,756 + Dsui_0692 0.21 -2.2 687,786 + Dsui_0692 0.24 -1.2 687,856 - Dsui_0692 0.32 -0.7 687,873 - Dsui_0692 0.34 +1.2
Or see this region's nucleotide sequence