Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0684 and Dsui_0685 are separated by 30 nucleotides Dsui_0685 and Dsui_0686 are separated by 8 nucleotides Dsui_0686 and Dsui_0687 overlap by 17 nucleotides Dsui_0687 and Dsui_0688 are separated by 95 nucleotides Dsui_0688 and Dsui_0689 overlap by 1 nucleotides
Dsui_0684: Dsui_0684 - putative nucleoside-diphosphate sugar epimerase, at 682,383 to 683,342
_0684
Dsui_0685: Dsui_0685 - glutathione S-transferase, at 683,373 to 684,008
_0685
Dsui_0686: Dsui_0686 - tRNA nucleotidyltransferase/poly(A) polymerase, at 684,017 to 685,237
_0686
Dsui_0687: Dsui_0687 - Protein of unknown function (DUF2905), at 685,221 to 685,412
_0687
Dsui_0688: Dsui_0688 - glutathione S-transferase, at 685,508 to 686,125
_0688
Dsui_0689: Dsui_0689 - lysophospholipase, at 686,125 to 686,937
_0689
Position (kb) 684
685
686 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 683.019 kb on - strand, within Dsui_0684 at 683.019 kb on - strand, within Dsui_0684 at 683.023 kb on + strand, within Dsui_0684 at 683.031 kb on - strand, within Dsui_0684 at 683.031 kb on - strand, within Dsui_0684 at 683.043 kb on + strand, within Dsui_0684 at 683.046 kb on - strand, within Dsui_0684 at 683.047 kb on + strand, within Dsui_0684 at 683.135 kb on + strand, within Dsui_0684 at 683.278 kb on + strand at 683.492 kb on + strand, within Dsui_0685 at 683.520 kb on + strand, within Dsui_0685 at 683.800 kb on - strand at 683.830 kb on + strand, within Dsui_0685 at 683.899 kb on - strand, within Dsui_0685 at 685.268 kb on - strand, within Dsui_0687 at 685.274 kb on - strand, within Dsui_0687 at 685.318 kb on - strand, within Dsui_0687 at 685.379 kb on + strand, within Dsui_0687 at 685.387 kb on - strand, within Dsui_0687 at 685.422 kb on - strand at 685.493 kb on + strand at 685.501 kb on - strand at 685.513 kb on - strand at 685.514 kb on + strand at 685.525 kb on - strand at 685.574 kb on + strand, within Dsui_0688 at 685.579 kb on + strand, within Dsui_0688 at 685.579 kb on + strand, within Dsui_0688 at 685.579 kb on + strand, within Dsui_0688 at 685.579 kb on + strand, within Dsui_0688 at 685.587 kb on - strand at 685.587 kb on - strand, within Dsui_0688 at 685.587 kb on - strand, within Dsui_0688 at 685.587 kb on - strand, within Dsui_0688 at 685.587 kb on - strand, within Dsui_0688 at 685.587 kb on - strand at 685.616 kb on - strand at 685.616 kb on - strand, within Dsui_0688 at 685.641 kb on - strand, within Dsui_0688 at 685.701 kb on + strand, within Dsui_0688 at 685.776 kb on + strand, within Dsui_0688 at 685.877 kb on - strand, within Dsui_0688 at 685.921 kb on + strand, within Dsui_0688 at 685.921 kb on + strand, within Dsui_0688 at 685.940 kb on + strand, within Dsui_0688 at 685.952 kb on + strand, within Dsui_0688 at 685.952 kb on + strand, within Dsui_0688 at 686.001 kb on + strand, within Dsui_0688 at 686.106 kb on + strand at 686.114 kb on - strand at 686.114 kb on - strand at 686.190 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 683,019 - Dsui_0684 0.66 -0.6 683,019 - Dsui_0684 0.66 +0.4 683,023 + Dsui_0684 0.67 -0.0 683,031 - Dsui_0684 0.68 +1.1 683,031 - Dsui_0684 0.68 +0.4 683,043 + Dsui_0684 0.69 -0.1 683,046 - Dsui_0684 0.69 -1.6 683,047 + Dsui_0684 0.69 -0.0 683,135 + Dsui_0684 0.78 +0.1 683,278 + +0.1 683,492 + Dsui_0685 0.19 +0.3 683,520 + Dsui_0685 0.23 -0.4 683,800 - +0.4 683,830 + Dsui_0685 0.72 -0.1 683,899 - Dsui_0685 0.83 -1.6 685,268 - Dsui_0687 0.24 -0.5 685,274 - Dsui_0687 0.28 +0.2 685,318 - Dsui_0687 0.51 +0.7 685,379 + Dsui_0687 0.82 -0.9 685,387 - Dsui_0687 0.86 +1.2 685,422 - +0.1 685,493 + -1.3 685,501 - -1.2 685,513 - -1.2 685,514 + -3.2 685,525 - -1.6 685,574 + Dsui_0688 0.11 -3.8 685,579 + Dsui_0688 0.11 +2.5 685,579 + Dsui_0688 0.11 +0.8 685,579 + Dsui_0688 0.11 +0.8 685,579 + Dsui_0688 0.11 -2.8 685,587 - +0.4 685,587 - Dsui_0688 0.13 -2.2 685,587 - Dsui_0688 0.13 -0.6 685,587 - Dsui_0688 0.13 -2.0 685,587 - Dsui_0688 0.13 -2.0 685,587 - -0.6 685,616 - +0.4 685,616 - Dsui_0688 0.17 -2.0 685,641 - Dsui_0688 0.22 -2.2 685,701 + Dsui_0688 0.31 -0.4 685,776 + Dsui_0688 0.43 -1.1 685,877 - Dsui_0688 0.60 +0.4 685,921 + Dsui_0688 0.67 -0.8 685,921 + Dsui_0688 0.67 +0.2 685,940 + Dsui_0688 0.70 -0.9 685,952 + Dsui_0688 0.72 -0.3 685,952 + Dsui_0688 0.72 -1.9 686,001 + Dsui_0688 0.80 -0.4 686,106 + -2.2 686,114 - -1.6 686,114 - -1.6 686,190 + +0.1
Or see this region's nucleotide sequence