Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0681 and Dsui_0682 are separated by 161 nucleotides Dsui_0682 and Dsui_0683 are separated by 161 nucleotides Dsui_0683 and Dsui_0684 are separated by 105 nucleotides
Dsui_0681: Dsui_0681 - ABC-type Fe3+-hydroxamate transport system, periplasmic component, at 678,563 to 679,372
_0681
Dsui_0682: Dsui_0682 - 5,10-methenyltetrahydrofolate synthetase, at 679,534 to 680,127
_0682
Dsui_0683: Dsui_0683 - soluble lytic murein transglycosylase-like protein, at 680,289 to 682,277
_0683
Dsui_0684: Dsui_0684 - putative nucleoside-diphosphate sugar epimerase, at 682,383 to 683,342
_0684
Position (kb)
680
681
682
683 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 679.331 kb on + strand at 679.339 kb on - strand at 680.121 kb on - strand at 680.173 kb on + strand at 680.181 kb on - strand at 680.186 kb on - strand at 680.254 kb on + strand at 680.257 kb on - strand at 680.299 kb on + strand at 680.306 kb on - strand at 680.338 kb on + strand at 680.346 kb on - strand at 680.502 kb on - strand, within Dsui_0683 at 680.525 kb on + strand, within Dsui_0683 at 680.545 kb on - strand at 680.593 kb on - strand at 680.642 kb on + strand, within Dsui_0683 at 680.738 kb on + strand, within Dsui_0683 at 680.804 kb on - strand, within Dsui_0683 at 680.816 kb on - strand, within Dsui_0683 at 680.899 kb on - strand at 680.913 kb on + strand, within Dsui_0683 at 680.913 kb on + strand, within Dsui_0683 at 680.913 kb on + strand, within Dsui_0683 at 680.918 kb on + strand, within Dsui_0683 at 681.007 kb on - strand, within Dsui_0683 at 681.150 kb on + strand, within Dsui_0683 at 681.239 kb on + strand, within Dsui_0683 at 681.276 kb on - strand at 681.312 kb on - strand, within Dsui_0683 at 681.386 kb on + strand, within Dsui_0683 at 681.526 kb on + strand, within Dsui_0683 at 681.593 kb on + strand, within Dsui_0683 at 681.671 kb on - strand, within Dsui_0683 at 681.727 kb on + strand, within Dsui_0683 at 681.757 kb on - strand, within Dsui_0683 at 681.861 kb on - strand, within Dsui_0683 at 681.948 kb on - strand, within Dsui_0683 at 681.952 kb on + strand, within Dsui_0683 at 681.962 kb on + strand, within Dsui_0683 at 681.991 kb on + strand, within Dsui_0683 at 682.015 kb on - strand, within Dsui_0683 at 682.168 kb on - strand at 682.198 kb on - strand at 682.234 kb on - strand at 682.308 kb on + strand at 682.328 kb on - strand at 682.355 kb on - strand at 682.356 kb on + strand at 682.361 kb on - strand at 682.369 kb on + strand at 682.377 kb on - strand at 682.518 kb on + strand, within Dsui_0684 at 682.664 kb on + strand, within Dsui_0684 at 682.672 kb on - strand at 682.750 kb on - strand, within Dsui_0684 at 682.768 kb on + strand, within Dsui_0684 at 682.881 kb on + strand, within Dsui_0684 at 682.891 kb on + strand, within Dsui_0684 at 682.899 kb on - strand, within Dsui_0684 at 682.901 kb on + strand, within Dsui_0684 at 682.909 kb on - strand, within Dsui_0684 at 682.914 kb on - strand, within Dsui_0684 at 682.917 kb on + strand, within Dsui_0684 at 682.974 kb on + strand, within Dsui_0684 at 683.001 kb on - strand at 683.011 kb on + strand, within Dsui_0684 at 683.019 kb on - strand, within Dsui_0684 at 683.019 kb on - strand, within Dsui_0684 at 683.023 kb on + strand, within Dsui_0684 at 683.031 kb on - strand, within Dsui_0684 at 683.031 kb on - strand, within Dsui_0684 at 683.043 kb on + strand, within Dsui_0684 at 683.046 kb on - strand, within Dsui_0684 at 683.047 kb on + strand, within Dsui_0684 at 683.135 kb on + strand, within Dsui_0684
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 679,331 + +0.7 679,339 - -3.1 680,121 - -0.4 680,173 + -0.6 680,181 - +0.6 680,186 - +0.3 680,254 + +0.6 680,257 - -0.7 680,299 + -2.7 680,306 - -1.2 680,338 + -2.9 680,346 - -0.6 680,502 - Dsui_0683 0.11 -1.2 680,525 + Dsui_0683 0.12 -2.4 680,545 - +0.4 680,593 - +0.4 680,642 + Dsui_0683 0.18 -2.4 680,738 + Dsui_0683 0.23 -1.4 680,804 - Dsui_0683 0.26 +0.4 680,816 - Dsui_0683 0.26 -1.6 680,899 - +0.4 680,913 + Dsui_0683 0.31 -0.9 680,913 + Dsui_0683 0.31 -2.2 680,913 + Dsui_0683 0.31 -2.9 680,918 + Dsui_0683 0.32 -3.0 681,007 - Dsui_0683 0.36 -1.6 681,150 + Dsui_0683 0.43 -1.8 681,239 + Dsui_0683 0.48 -2.5 681,276 - +0.4 681,312 - Dsui_0683 0.51 -0.6 681,386 + Dsui_0683 0.55 -3.2 681,526 + Dsui_0683 0.62 -2.3 681,593 + Dsui_0683 0.66 -2.7 681,671 - Dsui_0683 0.69 -0.6 681,727 + Dsui_0683 0.72 -2.7 681,757 - Dsui_0683 0.74 -0.6 681,861 - Dsui_0683 0.79 -1.2 681,948 - Dsui_0683 0.83 -0.6 681,952 + Dsui_0683 0.84 -2.8 681,962 + Dsui_0683 0.84 -0.9 681,991 + Dsui_0683 0.86 -1.7 682,015 - Dsui_0683 0.87 -0.6 682,168 - -0.6 682,198 - -2.2 682,234 - -0.6 682,308 + +0.1 682,328 - -0.6 682,355 - -0.6 682,356 + -0.3 682,361 - -0.3 682,369 + +0.1 682,377 - -0.6 682,518 + Dsui_0684 0.14 -0.2 682,664 + Dsui_0684 0.29 +0.5 682,672 - +0.4 682,750 - Dsui_0684 0.38 -1.6 682,768 + Dsui_0684 0.40 +0.3 682,881 + Dsui_0684 0.52 -0.1 682,891 + Dsui_0684 0.53 -0.3 682,899 - Dsui_0684 0.54 -0.6 682,901 + Dsui_0684 0.54 +1.0 682,909 - Dsui_0684 0.55 -2.2 682,914 - Dsui_0684 0.55 +1.0 682,917 + Dsui_0684 0.56 +0.7 682,974 + Dsui_0684 0.62 -0.2 683,001 - +0.4 683,011 + Dsui_0684 0.65 +0.4 683,019 - Dsui_0684 0.66 -0.6 683,019 - Dsui_0684 0.66 +0.4 683,023 + Dsui_0684 0.67 -0.0 683,031 - Dsui_0684 0.68 +1.1 683,031 - Dsui_0684 0.68 +0.4 683,043 + Dsui_0684 0.69 -0.1 683,046 - Dsui_0684 0.69 -1.6 683,047 + Dsui_0684 0.69 -0.0 683,135 + Dsui_0684 0.78 +0.1
Or see this region's nucleotide sequence