Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0673 and Dsui_0674 are separated by 34 nucleotides Dsui_0674 and Dsui_0675 are separated by 11 nucleotides Dsui_0675 and Dsui_0676 are separated by 77 nucleotides
Dsui_0673: Dsui_0673 - arginyl-tRNA synthetase, at 671,862 to 673,604
_0673
Dsui_0674: Dsui_0674 - cell division protein, at 673,639 to 674,295
_0674
Dsui_0675: Dsui_0675 - putative dithiol-disulfide isomerase involved in polyketide biosynthesis, at 674,307 to 674,966
_0675
Dsui_0676: Dsui_0676 - short-chain dehydrogenase of unknown substrate specificity, at 675,044 to 675,817
_0676
Position (kb) 673
674
675 Strain fitness (log2 ratio) -2
-1
0
1
2 at 673.617 kb on + strand at 673.617 kb on + strand at 673.629 kb on + strand at 673.636 kb on + strand at 674.250 kb on + strand at 674.250 kb on + strand at 674.312 kb on - strand at 674.336 kb on - strand at 674.400 kb on + strand, within Dsui_0675 at 674.469 kb on - strand at 674.516 kb on - strand at 674.595 kb on + strand, within Dsui_0675 at 674.708 kb on + strand, within Dsui_0675 at 674.878 kb on - strand at 674.884 kb on - strand at 674.906 kb on + strand at 675.059 kb on - strand at 675.060 kb on - strand at 675.067 kb on - strand at 675.067 kb on - strand at 675.137 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 673,617 + -0.1 673,617 + +0.3 673,629 + +0.6 673,636 + -0.3 674,250 + -1.1 674,250 + -1.5 674,312 - -1.5 674,336 - +0.4 674,400 + Dsui_0675 0.14 -2.0 674,469 - +0.4 674,516 - -0.6 674,595 + Dsui_0675 0.44 -2.1 674,708 + Dsui_0675 0.61 -2.4 674,878 - -0.6 674,884 - -1.2 674,906 + +0.1 675,059 - +0.8 675,060 - +0.4 675,067 - +0.1 675,067 - +0.6 675,137 - +1.9
Or see this region's nucleotide sequence