Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0656 and Dsui_0657 are separated by 33 nucleotides Dsui_0657 and Dsui_0658 are separated by 79 nucleotides Dsui_0658 and Dsui_0659 are separated by 14 nucleotides Dsui_0659 and Dsui_0660 are separated by 21 nucleotides
Dsui_0656: Dsui_0656 - chemotaxis protein histidine kinase-like protein, at 649,135 to 655,254
_0656
Dsui_0657: Dsui_0657 - methyl-accepting chemotaxis protein, at 655,288 to 657,486
_0657
Dsui_0658: Dsui_0658 - chemotaxis signal transduction protein, at 657,566 to 658,078
_0658
Dsui_0659: Dsui_0659 - response regulator with CheY-like receiver domain and winged-helix DNA-binding domain, at 658,093 to 658,455
_0659
Dsui_0660: Dsui_0660 - response regulator with CheY-like receiver domain and winged-helix DNA-binding domain, at 658,477 to 658,875
_0660
Position (kb) 655
656
657
658 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 654.356 kb on + strand, within Dsui_0656 at 654.402 kb on + strand at 654.414 kb on + strand at 654.589 kb on - strand, within Dsui_0656 at 654.758 kb on + strand at 654.791 kb on + strand at 654.824 kb on + strand at 654.882 kb on + strand at 655.011 kb on + strand at 655.022 kb on + strand at 655.051 kb on - strand at 655.070 kb on - strand at 655.213 kb on - strand at 655.224 kb on - strand at 655.252 kb on + strand at 655.304 kb on - strand at 655.304 kb on - strand at 655.384 kb on + strand at 655.491 kb on + strand at 655.749 kb on + strand, within Dsui_0657 at 655.817 kb on - strand, within Dsui_0657 at 655.817 kb on - strand, within Dsui_0657 at 655.868 kb on - strand, within Dsui_0657 at 655.901 kb on + strand at 655.920 kb on - strand, within Dsui_0657 at 655.996 kb on + strand, within Dsui_0657 at 656.035 kb on - strand, within Dsui_0657 at 656.085 kb on - strand, within Dsui_0657 at 656.089 kb on + strand, within Dsui_0657 at 656.102 kb on - strand, within Dsui_0657 at 656.131 kb on - strand, within Dsui_0657 at 656.137 kb on - strand, within Dsui_0657 at 656.220 kb on - strand, within Dsui_0657 at 656.350 kb on + strand, within Dsui_0657 at 656.351 kb on + strand, within Dsui_0657 at 656.465 kb on - strand, within Dsui_0657 at 656.486 kb on - strand, within Dsui_0657 at 656.593 kb on - strand, within Dsui_0657 at 656.638 kb on - strand, within Dsui_0657 at 656.783 kb on - strand, within Dsui_0657 at 656.788 kb on - strand, within Dsui_0657 at 656.863 kb on - strand, within Dsui_0657 at 656.891 kb on + strand, within Dsui_0657 at 656.897 kb on + strand, within Dsui_0657 at 656.905 kb on - strand, within Dsui_0657 at 656.974 kb on + strand, within Dsui_0657 at 657.064 kb on - strand, within Dsui_0657 at 657.089 kb on + strand, within Dsui_0657 at 657.140 kb on + strand at 657.247 kb on - strand, within Dsui_0657 at 657.267 kb on - strand at 657.362 kb on + strand at 657.602 kb on + strand at 657.619 kb on + strand at 657.662 kb on + strand, within Dsui_0658 at 657.791 kb on - strand, within Dsui_0658 at 657.866 kb on - strand, within Dsui_0658 at 657.966 kb on - strand, within Dsui_0658 at 658.090 kb on + strand at 658.096 kb on - strand at 658.251 kb on + strand at 658.316 kb on - strand, within Dsui_0659 at 658.328 kb on + strand at 658.457 kb on + strand at 658.458 kb on - strand at 658.459 kb on - strand at 658.486 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 654,356 + Dsui_0656 0.85 +1.6 654,402 + -1.2 654,414 + +4.1 654,589 - Dsui_0656 0.89 +2.3 654,758 + +4.3 654,791 + +2.0 654,824 + +2.4 654,882 + +4.5 655,011 + +4.1 655,022 + +3.4 655,051 - +2.7 655,070 - +4.8 655,213 - +1.7 655,224 - +3.5 655,252 + +2.9 655,304 - -2.1 655,304 - -2.9 655,384 + +5.1 655,491 + +2.4 655,749 + Dsui_0657 0.21 +3.4 655,817 - Dsui_0657 0.24 +4.6 655,817 - Dsui_0657 0.24 +3.5 655,868 - Dsui_0657 0.26 +4.1 655,901 + +2.8 655,920 - Dsui_0657 0.29 +4.2 655,996 + Dsui_0657 0.32 +4.3 656,035 - Dsui_0657 0.34 +4.0 656,085 - Dsui_0657 0.36 +4.4 656,089 + Dsui_0657 0.36 +3.6 656,102 - Dsui_0657 0.37 +4.1 656,131 - Dsui_0657 0.38 +4.1 656,137 - Dsui_0657 0.39 +4.3 656,220 - Dsui_0657 0.42 +3.6 656,350 + Dsui_0657 0.48 -0.6 656,351 + Dsui_0657 0.48 +3.1 656,465 - Dsui_0657 0.54 +4.9 656,486 - Dsui_0657 0.54 +4.3 656,593 - Dsui_0657 0.59 +4.0 656,638 - Dsui_0657 0.61 +4.1 656,783 - Dsui_0657 0.68 +4.5 656,788 - Dsui_0657 0.68 +5.2 656,863 - Dsui_0657 0.72 +5.0 656,891 + Dsui_0657 0.73 +1.4 656,897 + Dsui_0657 0.73 +0.4 656,905 - Dsui_0657 0.74 +4.5 656,974 + Dsui_0657 0.77 +2.0 657,064 - Dsui_0657 0.81 +3.7 657,089 + Dsui_0657 0.82 +0.4 657,140 + +5.0 657,247 - Dsui_0657 0.89 +3.8 657,267 - +4.6 657,362 + -0.6 657,602 + +0.4 657,619 + +0.4 657,662 + Dsui_0658 0.19 -0.6 657,791 - Dsui_0658 0.44 +1.3 657,866 - Dsui_0658 0.58 +0.3 657,966 - Dsui_0658 0.78 +1.7 658,090 + -0.6 658,096 - -0.0 658,251 + +0.4 658,316 - Dsui_0659 0.61 +6.1 658,328 + +0.4 658,457 + +0.4 658,458 - +3.6 658,459 - +3.2 658,486 - +2.1
Or see this region's nucleotide sequence