Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0653 and Dsui_0654 are separated by 238 nucleotides Dsui_0654 and Dsui_0655 are separated by 125 nucleotides Dsui_0655 and Dsui_0656 are separated by 57 nucleotides
Dsui_0653: Dsui_0653 - putative SAM-dependent methyltransferase, at 646,444 to 647,631
_0653
Dsui_0654: Dsui_0654 - hypothetical protein, at 647,870 to 648,325
_0654
Dsui_0655: Dsui_0655 - putative membrane protein, at 648,451 to 649,077
_0655
Dsui_0656: Dsui_0656 - chemotaxis protein histidine kinase-like protein, at 649,135 to 655,254
_0656
Position (kb) 647
648
649 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 646.905 kb on - strand, within Dsui_0653 at 647.054 kb on + strand, within Dsui_0653 at 647.062 kb on - strand, within Dsui_0653 at 647.079 kb on + strand at 647.106 kb on - strand, within Dsui_0653 at 647.197 kb on + strand at 647.252 kb on - strand, within Dsui_0653 at 647.304 kb on + strand, within Dsui_0653 at 647.304 kb on + strand, within Dsui_0653 at 647.312 kb on - strand, within Dsui_0653 at 647.417 kb on + strand, within Dsui_0653 at 647.445 kb on + strand, within Dsui_0653 at 647.445 kb on + strand, within Dsui_0653 at 647.534 kb on + strand at 647.604 kb on + strand at 647.866 kb on + strand at 647.940 kb on - strand at 647.955 kb on - strand, within Dsui_0654 at 647.967 kb on + strand, within Dsui_0654 at 647.989 kb on - strand, within Dsui_0654 at 647.990 kb on - strand, within Dsui_0654 at 648.044 kb on - strand at 648.101 kb on + strand, within Dsui_0654 at 648.138 kb on + strand, within Dsui_0654 at 648.144 kb on - strand at 648.185 kb on + strand, within Dsui_0654 at 648.190 kb on + strand, within Dsui_0654 at 648.193 kb on - strand, within Dsui_0654 at 648.211 kb on + strand, within Dsui_0654 at 648.225 kb on + strand at 648.226 kb on - strand, within Dsui_0654 at 648.274 kb on + strand, within Dsui_0654 at 648.282 kb on - strand at 648.399 kb on + strand at 648.421 kb on - strand at 648.421 kb on - strand at 648.423 kb on + strand at 648.448 kb on + strand at 648.497 kb on + strand at 648.505 kb on - strand at 648.582 kb on + strand, within Dsui_0655 at 648.592 kb on + strand, within Dsui_0655 at 648.600 kb on - strand, within Dsui_0655 at 648.622 kb on + strand, within Dsui_0655 at 648.675 kb on + strand, within Dsui_0655 at 648.712 kb on - strand, within Dsui_0655 at 648.910 kb on + strand, within Dsui_0655 at 649.004 kb on - strand, within Dsui_0655 at 649.004 kb on - strand, within Dsui_0655 at 649.006 kb on + strand, within Dsui_0655 at 649.014 kb on - strand, within Dsui_0655 at 649.072 kb on + strand at 649.073 kb on + strand at 649.080 kb on - strand at 649.157 kb on + strand at 649.157 kb on + strand at 649.157 kb on + strand at 649.211 kb on - strand at 649.215 kb on + strand at 649.216 kb on - strand at 649.243 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 646,905 - Dsui_0653 0.39 -0.6 647,054 + Dsui_0653 0.51 +1.4 647,062 - Dsui_0653 0.52 +0.4 647,079 + +0.4 647,106 - Dsui_0653 0.56 -1.4 647,197 + +0.4 647,252 - Dsui_0653 0.68 -2.2 647,304 + Dsui_0653 0.72 -1.9 647,304 + Dsui_0653 0.72 -0.6 647,312 - Dsui_0653 0.73 -1.9 647,417 + Dsui_0653 0.82 -0.6 647,445 + Dsui_0653 0.84 -1.2 647,445 + Dsui_0653 0.84 -0.6 647,534 + +0.4 647,604 + -0.6 647,866 + +1.8 647,940 - +0.4 647,955 - Dsui_0654 0.19 -0.6 647,967 + Dsui_0654 0.21 -0.8 647,989 - Dsui_0654 0.26 -1.2 647,990 - Dsui_0654 0.26 -2.6 648,044 - -0.6 648,101 + Dsui_0654 0.51 -1.9 648,138 + Dsui_0654 0.59 -0.6 648,144 - -1.2 648,185 + Dsui_0654 0.69 +0.4 648,190 + Dsui_0654 0.70 -1.2 648,193 - Dsui_0654 0.71 -0.6 648,211 + Dsui_0654 0.75 +0.4 648,225 + +0.4 648,226 - Dsui_0654 0.78 +0.4 648,274 + Dsui_0654 0.89 -0.6 648,282 - +0.4 648,399 + +0.4 648,421 - -2.8 648,421 - +0.4 648,423 + +0.4 648,448 + -0.2 648,497 + -1.9 648,505 - -0.6 648,582 + Dsui_0655 0.21 +0.4 648,592 + Dsui_0655 0.22 +2.8 648,600 - Dsui_0655 0.24 -0.6 648,622 + Dsui_0655 0.27 -0.9 648,675 + Dsui_0655 0.36 +0.1 648,712 - Dsui_0655 0.42 -1.9 648,910 + Dsui_0655 0.73 -0.4 649,004 - Dsui_0655 0.88 -1.2 649,004 - Dsui_0655 0.88 -3.7 649,006 + Dsui_0655 0.89 -1.2 649,014 - Dsui_0655 0.90 +0.4 649,072 + +0.4 649,073 + -0.8 649,080 - +1.4 649,157 + -1.1 649,157 + -1.2 649,157 + +0.4 649,211 - -1.2 649,215 + -2.8 649,216 - -2.0 649,243 - -1.5
Or see this region's nucleotide sequence