Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0630 and Dsui_0631 are separated by 189 nucleotides Dsui_0631 and Dsui_0632 are separated by 50 nucleotides Dsui_0632 and Dsui_0633 are separated by 54 nucleotides
Dsui_0630: Dsui_0630 - ABC-type branched-chain amino acid transport system, periplasmic component, at 623,508 to 624,812
_0630
Dsui_0631: Dsui_0631 - cytochrome c551/c552, at 625,002 to 625,310
_0631
Dsui_0632: Dsui_0632 - thioredoxin domain-containing protein, at 625,361 to 627,409
_0632
Dsui_0633: Dsui_0633 - transcriptional regulator, at 627,464 to 628,369
_0633
Position (kb) 625
626
627
628 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 624.447 kb on - strand, within Dsui_0630 at 624.460 kb on + strand, within Dsui_0630 at 624.468 kb on - strand, within Dsui_0630 at 624.491 kb on - strand, within Dsui_0630 at 624.496 kb on + strand, within Dsui_0630 at 624.504 kb on - strand, within Dsui_0630 at 624.506 kb on + strand, within Dsui_0630 at 624.547 kb on - strand, within Dsui_0630 at 624.559 kb on - strand, within Dsui_0630 at 624.654 kb on + strand, within Dsui_0630 at 624.777 kb on + strand at 624.780 kb on - strand at 624.790 kb on - strand at 624.793 kb on + strand at 625.011 kb on + strand at 625.019 kb on - strand at 625.021 kb on + strand at 625.127 kb on - strand, within Dsui_0631 at 625.230 kb on + strand, within Dsui_0631 at 625.250 kb on + strand, within Dsui_0631 at 625.401 kb on + strand at 625.603 kb on + strand, within Dsui_0632 at 625.685 kb on - strand, within Dsui_0632 at 625.741 kb on - strand, within Dsui_0632 at 625.748 kb on + strand, within Dsui_0632 at 625.793 kb on + strand, within Dsui_0632 at 625.903 kb on + strand at 625.918 kb on + strand, within Dsui_0632 at 625.938 kb on + strand, within Dsui_0632 at 626.089 kb on - strand, within Dsui_0632 at 626.160 kb on + strand, within Dsui_0632 at 626.160 kb on + strand, within Dsui_0632 at 626.184 kb on + strand, within Dsui_0632 at 626.291 kb on + strand, within Dsui_0632 at 626.292 kb on + strand at 626.401 kb on - strand, within Dsui_0632 at 626.409 kb on - strand, within Dsui_0632 at 626.433 kb on + strand, within Dsui_0632 at 626.554 kb on + strand, within Dsui_0632 at 626.568 kb on + strand, within Dsui_0632 at 626.643 kb on - strand, within Dsui_0632 at 626.837 kb on + strand, within Dsui_0632 at 626.857 kb on + strand, within Dsui_0632 at 626.879 kb on - strand, within Dsui_0632 at 626.889 kb on - strand, within Dsui_0632 at 626.934 kb on + strand, within Dsui_0632 at 626.934 kb on + strand, within Dsui_0632 at 626.942 kb on - strand, within Dsui_0632 at 626.942 kb on - strand, within Dsui_0632 at 626.942 kb on - strand, within Dsui_0632 at 626.942 kb on - strand, within Dsui_0632 at 626.990 kb on + strand, within Dsui_0632 at 627.143 kb on - strand, within Dsui_0632 at 627.175 kb on + strand, within Dsui_0632 at 627.241 kb on - strand at 627.261 kb on - strand at 627.381 kb on - strand at 627.462 kb on + strand at 627.497 kb on - strand at 627.627 kb on - strand, within Dsui_0633 at 627.673 kb on - strand, within Dsui_0633 at 627.741 kb on - strand, within Dsui_0633 at 627.883 kb on - strand, within Dsui_0633 at 627.943 kb on - strand, within Dsui_0633 at 627.988 kb on + strand, within Dsui_0633 at 627.996 kb on - strand, within Dsui_0633 at 627.996 kb on - strand, within Dsui_0633 at 627.996 kb on - strand, within Dsui_0633 at 627.999 kb on + strand, within Dsui_0633 at 628.004 kb on + strand, within Dsui_0633 at 628.012 kb on - strand, within Dsui_0633 at 628.012 kb on - strand, within Dsui_0633 at 628.075 kb on - strand, within Dsui_0633 at 628.135 kb on + strand, within Dsui_0633 at 628.215 kb on + strand, within Dsui_0633 at 628.340 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 624,447 - Dsui_0630 0.72 -0.4 624,460 + Dsui_0630 0.73 -2.6 624,468 - Dsui_0630 0.74 -0.2 624,491 - Dsui_0630 0.75 -0.4 624,496 + Dsui_0630 0.76 -2.2 624,504 - Dsui_0630 0.76 +0.3 624,506 + Dsui_0630 0.76 +0.4 624,547 - Dsui_0630 0.80 -0.2 624,559 - Dsui_0630 0.81 -0.5 624,654 + Dsui_0630 0.88 -2.2 624,777 + +0.4 624,780 - -0.4 624,790 - +0.2 624,793 + +0.4 625,011 + +0.8 625,019 - -0.2 625,021 + -2.2 625,127 - Dsui_0631 0.40 +0.6 625,230 + Dsui_0631 0.74 +0.8 625,250 + Dsui_0631 0.80 -2.6 625,401 + -0.6 625,603 + Dsui_0632 0.12 -1.6 625,685 - Dsui_0632 0.16 +0.1 625,741 - Dsui_0632 0.19 -0.4 625,748 + Dsui_0632 0.19 -0.6 625,793 + Dsui_0632 0.21 -1.2 625,903 + +0.4 625,918 + Dsui_0632 0.27 -1.6 625,938 + Dsui_0632 0.28 +0.4 626,089 - Dsui_0632 0.36 +1.0 626,160 + Dsui_0632 0.39 -2.0 626,160 + Dsui_0632 0.39 +0.4 626,184 + Dsui_0632 0.40 -2.0 626,291 + Dsui_0632 0.45 +0.4 626,292 + +1.9 626,401 - Dsui_0632 0.51 -0.1 626,409 - Dsui_0632 0.51 -0.4 626,433 + Dsui_0632 0.52 -0.6 626,554 + Dsui_0632 0.58 +0.4 626,568 + Dsui_0632 0.59 -2.2 626,643 - Dsui_0632 0.63 -0.8 626,837 + Dsui_0632 0.72 -1.2 626,857 + Dsui_0632 0.73 -3.0 626,879 - Dsui_0632 0.74 +0.1 626,889 - Dsui_0632 0.75 -0.2 626,934 + Dsui_0632 0.77 -2.0 626,934 + Dsui_0632 0.77 -3.2 626,942 - Dsui_0632 0.77 -0.3 626,942 - Dsui_0632 0.77 -1.2 626,942 - Dsui_0632 0.77 +0.4 626,942 - Dsui_0632 0.77 -0.6 626,990 + Dsui_0632 0.80 +0.4 627,143 - Dsui_0632 0.87 -0.5 627,175 + Dsui_0632 0.89 -1.6 627,241 - -0.4 627,261 - -0.2 627,381 - -0.1 627,462 + +0.4 627,497 - +0.5 627,627 - Dsui_0633 0.18 +0.1 627,673 - Dsui_0633 0.23 -0.8 627,741 - Dsui_0633 0.31 -0.4 627,883 - Dsui_0633 0.46 -0.9 627,943 - Dsui_0633 0.53 -0.1 627,988 + Dsui_0633 0.58 +0.4 627,996 - Dsui_0633 0.59 +0.4 627,996 - Dsui_0633 0.59 +0.8 627,996 - Dsui_0633 0.59 -0.4 627,999 + Dsui_0633 0.59 -1.2 628,004 + Dsui_0633 0.60 +0.4 628,012 - Dsui_0633 0.60 +0.2 628,012 - Dsui_0633 0.60 -0.4 628,075 - Dsui_0633 0.67 -0.3 628,135 + Dsui_0633 0.74 +0.4 628,215 + Dsui_0633 0.83 -0.6 628,340 - +0.5
Or see this region's nucleotide sequence