Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0593 and Dsui_0594 overlap by 8 nucleotides Dsui_0594 and Dsui_0595 overlap by 8 nucleotides Dsui_0595 and Dsui_0596 overlap by 8 nucleotides Dsui_0596 and Dsui_0597 are separated by 5 nucleotides
Dsui_0593: Dsui_0593 - putative transcriptional regulator, at 591,341 to 591,901
_0593
Dsui_0594: Dsui_0594 - RNAse H-fold protein YqgF, at 591,894 to 592,370
_0594
Dsui_0595: Dsui_0595 - pyrimidine operon attenuation protein/uracil phosphoribosyltransferase, at 592,363 to 592,884
_0595
Dsui_0596: Dsui_0596 - aspartate carbamoyltransferase, at 592,877 to 593,860
_0596
Dsui_0597: Dsui_0597 - dihydroorotase, multifunctional complex type, at 593,866 to 595,110
_0597
Position (kb) 592
593 Strain fitness (log2 ratio) -1
0
1 at 591.466 kb on + strand, within Dsui_0593 at 591.586 kb on - strand, within Dsui_0593 at 591.647 kb on + strand, within Dsui_0593 at 591.721 kb on + strand, within Dsui_0593 at 591.724 kb on - strand, within Dsui_0593 at 591.895 kb on + strand at 591.896 kb on + strand at 592.472 kb on + strand, within Dsui_0595 at 592.604 kb on + strand, within Dsui_0595 at 592.644 kb on + strand, within Dsui_0595 at 592.656 kb on + strand, within Dsui_0595
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 591,466 + Dsui_0593 0.22 -0.5 591,586 - Dsui_0593 0.44 +0.4 591,647 + Dsui_0593 0.55 -0.3 591,721 + Dsui_0593 0.68 -0.2 591,724 - Dsui_0593 0.68 +1.4 591,895 + +0.0 591,896 + +0.4 592,472 + Dsui_0595 0.21 -0.7 592,604 + Dsui_0595 0.46 -0.8 592,644 + Dsui_0595 0.54 -0.1 592,656 + Dsui_0595 0.56 -0.3
Or see this region's nucleotide sequence