Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0500 and Dsui_0501 are separated by 5 nucleotides Dsui_0501 and Dsui_0502 are separated by 34 nucleotides Dsui_0502 and Dsui_0503 are separated by 46 nucleotides
Dsui_0500: Dsui_0500 - (p)ppGpp synthetase, RelA/SpoT family, at 501,253 to 503,484
_0500
Dsui_0501: Dsui_0501 - glycine/D-amino acid oxidase, deaminating, at 503,490 to 504,740
_0501
Dsui_0502: Dsui_0502 - enoyl-CoA hydratase/carnithine racemase, at 504,775 to 505,566
_0502
Dsui_0503: Dsui_0503 - Mg-dependent DNase, at 505,613 to 506,473
_0503
Position (kb) 503
504
505 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 502.502 kb on - strand, within Dsui_0500 at 502.502 kb on - strand, within Dsui_0500 at 503.002 kb on + strand, within Dsui_0500 at 503.066 kb on + strand, within Dsui_0500 at 504.780 kb on - strand at 504.780 kb on - strand at 504.808 kb on + strand at 504.828 kb on - strand at 504.830 kb on + strand at 504.945 kb on + strand, within Dsui_0502 at 505.044 kb on + strand, within Dsui_0502 at 505.052 kb on - strand, within Dsui_0502 at 505.060 kb on - strand, within Dsui_0502 at 505.244 kb on + strand, within Dsui_0502 at 505.325 kb on - strand, within Dsui_0502 at 505.340 kb on - strand at 505.407 kb on - strand, within Dsui_0502 at 505.410 kb on + strand, within Dsui_0502 at 505.410 kb on + strand, within Dsui_0502 at 505.618 kb on + strand at 505.713 kb on - strand at 505.713 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 502,502 - Dsui_0500 0.56 +3.0 502,502 - Dsui_0500 0.56 +3.7 503,002 + Dsui_0500 0.78 +4.5 503,066 + Dsui_0500 0.81 +5.1 504,780 - +0.4 504,780 - +0.4 504,808 + +0.4 504,828 - -0.5 504,830 + -0.2 504,945 + Dsui_0502 0.21 -0.3 505,044 + Dsui_0502 0.34 -0.3 505,052 - Dsui_0502 0.35 +0.4 505,060 - Dsui_0502 0.36 -2.6 505,244 + Dsui_0502 0.59 -0.3 505,325 - Dsui_0502 0.69 -2.1 505,340 - -0.6 505,407 - Dsui_0502 0.80 -0.2 505,410 + Dsui_0502 0.80 -0.1 505,410 + Dsui_0502 0.80 +1.1 505,618 + -0.2 505,713 - +0.4 505,713 - +0.4
Or see this region's nucleotide sequence