Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0463 and Dsui_0464 are separated by 6 nucleotides Dsui_0464 and Dsui_0465 overlap by 14 nucleotides Dsui_0465 and Dsui_0466 overlap by 26 nucleotides
Dsui_0463: Dsui_0463 - hypothetical protein, at 468,986 to 470,323
_0463
Dsui_0464: Dsui_0464 - hypothetical protein, at 470,330 to 471,286
_0464
Dsui_0465: Dsui_0465 - ADP-heptose:LPS heptosyltransferase, at 471,273 to 472,451
_0465
Dsui_0466: Dsui_0466 - glycosyltransferase, at 472,426 to 473,532
_0466
Position (kb) 471
472
473 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 470.275 kb on - strand at 470.366 kb on + strand at 470.446 kb on - strand, within Dsui_0464 at 470.679 kb on + strand, within Dsui_0464 at 470.707 kb on - strand, within Dsui_0464 at 470.757 kb on + strand at 470.758 kb on - strand, within Dsui_0464 at 470.818 kb on - strand, within Dsui_0464 at 470.893 kb on + strand, within Dsui_0464 at 470.918 kb on + strand, within Dsui_0464 at 470.973 kb on - strand, within Dsui_0464 at 471.108 kb on - strand, within Dsui_0464 at 471.157 kb on + strand at 471.168 kb on - strand, within Dsui_0464 at 471.236 kb on - strand at 471.271 kb on + strand at 471.439 kb on + strand at 471.683 kb on - strand, within Dsui_0465 at 471.761 kb on - strand, within Dsui_0465 at 471.786 kb on - strand, within Dsui_0465 at 472.212 kb on - strand, within Dsui_0465
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 470,275 - +1.0 470,366 + +0.7 470,446 - Dsui_0464 0.12 +0.3 470,679 + Dsui_0464 0.36 -3.1 470,707 - Dsui_0464 0.39 +0.3 470,757 + +0.4 470,758 - Dsui_0464 0.45 +0.9 470,818 - Dsui_0464 0.51 +0.1 470,893 + Dsui_0464 0.59 -0.6 470,918 + Dsui_0464 0.61 -0.6 470,973 - Dsui_0464 0.67 +1.7 471,108 - Dsui_0464 0.81 -0.1 471,157 + +0.4 471,168 - Dsui_0464 0.88 +0.4 471,236 - -0.1 471,271 + +0.4 471,439 + +0.4 471,683 - Dsui_0465 0.35 +1.3 471,761 - Dsui_0465 0.41 +1.3 471,786 - Dsui_0465 0.44 +2.3 472,212 - Dsui_0465 0.80 +1.6
Or see this region's nucleotide sequence