Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0457 and Dsui_0458 are separated by 44 nucleotides Dsui_0458 and Dsui_0459 are separated by 26 nucleotides Dsui_0459 and Dsui_0460 are separated by 25 nucleotides Dsui_0460 and Dsui_0461 overlap by 4 nucleotides
Dsui_0457: Dsui_0457 - Protein of unknown function (DUF2782), at 461,826 to 462,176
_0457
Dsui_0458: Dsui_0458 - TIGR00730 family protein, at 462,221 to 462,949
_0458
Dsui_0459: Dsui_0459 - DNA polymerase I, at 462,976 to 465,708
_0459
Dsui_0460: Dsui_0460 - ADP-heptose:LPS heptosyltransferase, at 465,734 to 466,699
_0460
Dsui_0461: Dsui_0461 - glycosyltransferase, at 466,696 to 467,832
_0461
Position (kb) 462
463
464
465
466 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 462.132 kb on + strand, within Dsui_0457 at 462.243 kb on - strand at 462.254 kb on - strand at 462.259 kb on + strand at 462.267 kb on - strand at 462.273 kb on + strand at 462.329 kb on + strand, within Dsui_0458 at 462.334 kb on + strand, within Dsui_0458 at 462.334 kb on + strand at 462.342 kb on - strand, within Dsui_0458 at 462.342 kb on - strand, within Dsui_0458 at 462.492 kb on - strand, within Dsui_0458 at 462.517 kb on + strand at 462.525 kb on - strand, within Dsui_0458 at 462.601 kb on - strand, within Dsui_0458 at 462.728 kb on - strand, within Dsui_0458 at 462.754 kb on + strand, within Dsui_0458 at 462.762 kb on - strand, within Dsui_0458 at 462.774 kb on + strand at 462.791 kb on + strand, within Dsui_0458 at 462.819 kb on + strand at 462.827 kb on - strand, within Dsui_0458 at 462.887 kb on - strand at 462.914 kb on - strand at 462.917 kb on + strand at 463.824 kb on + strand, within Dsui_0459 at 464.259 kb on + strand, within Dsui_0459 at 465.115 kb on + strand, within Dsui_0459 at 465.178 kb on - strand at 465.213 kb on + strand, within Dsui_0459 at 465.545 kb on + strand at 465.812 kb on + strand at 465.820 kb on - strand at 465.888 kb on - strand, within Dsui_0460 at 465.940 kb on - strand, within Dsui_0460 at 466.153 kb on + strand at 466.448 kb on + strand, within Dsui_0460 at 466.642 kb on + strand at 466.650 kb on + strand at 466.650 kb on + strand at 466.650 kb on + strand at 466.650 kb on + strand at 466.652 kb on + strand at 466.658 kb on - strand at 466.658 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 462,132 + Dsui_0457 0.87 +0.4 462,243 - -0.5 462,254 - -0.4 462,259 + -1.2 462,267 - -0.8 462,273 + -0.6 462,329 + Dsui_0458 0.15 -1.2 462,334 + Dsui_0458 0.16 -1.6 462,334 + +0.4 462,342 - Dsui_0458 0.17 +0.8 462,342 - Dsui_0458 0.17 +0.4 462,492 - Dsui_0458 0.37 +0.0 462,517 + +0.4 462,525 - Dsui_0458 0.42 -1.5 462,601 - Dsui_0458 0.52 +0.6 462,728 - Dsui_0458 0.70 -0.1 462,754 + Dsui_0458 0.73 -1.6 462,762 - Dsui_0458 0.74 -0.4 462,774 + +0.4 462,791 + Dsui_0458 0.78 -0.6 462,819 + +0.4 462,827 - Dsui_0458 0.83 +0.2 462,887 - -0.5 462,914 - -0.7 462,917 + +0.6 463,824 + Dsui_0459 0.31 +0.5 464,259 + Dsui_0459 0.47 -2.0 465,115 + Dsui_0459 0.78 +0.0 465,178 - +0.4 465,213 + Dsui_0459 0.82 -1.6 465,545 + -1.3 465,812 + +3.8 465,820 - +3.9 465,888 - Dsui_0460 0.16 +3.8 465,940 - Dsui_0460 0.21 +4.2 466,153 + -0.6 466,448 + Dsui_0460 0.74 +0.4 466,642 + -1.9 466,650 + -0.6 466,650 + +0.4 466,650 + +0.4 466,650 + +0.4 466,652 + -2.6 466,658 - +3.9 466,658 - +4.2
Or see this region's nucleotide sequence