Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0424 and Dsui_0425 overlap by 4 nucleotides Dsui_0425 and Dsui_0426 are separated by 6 nucleotides
Dsui_0424: Dsui_0424 - phosphoketolase, at 427,689 to 430,109
_0424
Dsui_0425: Dsui_0425 - putative Fe-S oxidoreductase, at 430,106 to 430,381
_0425
Dsui_0426: Dsui_0426 - transcriptional regulator, at 430,388 to 431,302
_0426
Position (kb) 430
431 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 429.124 kb on + strand, within Dsui_0424 at 429.288 kb on - strand at 429.306 kb on - strand, within Dsui_0424 at 429.352 kb on + strand, within Dsui_0424 at 429.381 kb on + strand, within Dsui_0424 at 429.384 kb on - strand, within Dsui_0424 at 429.442 kb on + strand, within Dsui_0424 at 429.522 kb on + strand, within Dsui_0424 at 429.676 kb on + strand, within Dsui_0424 at 429.715 kb on + strand, within Dsui_0424 at 429.758 kb on + strand, within Dsui_0424 at 429.758 kb on + strand, within Dsui_0424 at 429.776 kb on - strand, within Dsui_0424 at 429.877 kb on + strand at 429.885 kb on - strand at 429.915 kb on - strand at 429.915 kb on - strand at 429.917 kb on + strand at 429.943 kb on + strand at 430.020 kb on - strand at 430.020 kb on - strand at 430.060 kb on + strand at 430.096 kb on - strand at 430.103 kb on + strand at 430.118 kb on + strand at 430.218 kb on + strand, within Dsui_0425 at 430.235 kb on + strand, within Dsui_0425 at 430.439 kb on + strand at 430.558 kb on + strand, within Dsui_0426 at 430.575 kb on + strand, within Dsui_0426 at 430.583 kb on - strand, within Dsui_0426 at 430.600 kb on - strand, within Dsui_0426 at 430.652 kb on + strand, within Dsui_0426 at 430.655 kb on - strand, within Dsui_0426 at 430.695 kb on - strand, within Dsui_0426 at 430.700 kb on - strand, within Dsui_0426 at 430.811 kb on - strand, within Dsui_0426 at 430.878 kb on - strand, within Dsui_0426 at 430.883 kb on - strand, within Dsui_0426 at 430.996 kb on - strand, within Dsui_0426 at 431.055 kb on + strand at 431.182 kb on + strand, within Dsui_0426 at 431.191 kb on - strand, within Dsui_0426 at 431.233 kb on - strand at 431.254 kb on - strand at 431.307 kb on - strand at 431.310 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 429,124 + Dsui_0424 0.59 +0.3 429,288 - +0.4 429,306 - Dsui_0424 0.67 -1.1 429,352 + Dsui_0424 0.69 -0.6 429,381 + Dsui_0424 0.70 -0.2 429,384 - Dsui_0424 0.70 +1.0 429,442 + Dsui_0424 0.72 +0.6 429,522 + Dsui_0424 0.76 -0.7 429,676 + Dsui_0424 0.82 -0.2 429,715 + Dsui_0424 0.84 +1.1 429,758 + Dsui_0424 0.85 +0.1 429,758 + Dsui_0424 0.85 +0.5 429,776 - Dsui_0424 0.86 +0.1 429,877 + +0.1 429,885 - -0.7 429,915 - +0.4 429,915 - -0.9 429,917 + +0.1 429,943 + +0.4 430,020 - -0.6 430,020 - +0.4 430,060 + +0.0 430,096 - -2.6 430,103 + -0.2 430,118 + +0.5 430,218 + Dsui_0425 0.41 +0.8 430,235 + Dsui_0425 0.47 +0.4 430,439 + +0.5 430,558 + Dsui_0426 0.19 -3.0 430,575 + Dsui_0426 0.20 +0.2 430,583 - Dsui_0426 0.21 -0.5 430,600 - Dsui_0426 0.23 +0.4 430,652 + Dsui_0426 0.29 +0.0 430,655 - Dsui_0426 0.29 -0.0 430,695 - Dsui_0426 0.34 -0.1 430,700 - Dsui_0426 0.34 +0.3 430,811 - Dsui_0426 0.46 +0.7 430,878 - Dsui_0426 0.54 -0.5 430,883 - Dsui_0426 0.54 +0.7 430,996 - Dsui_0426 0.66 -0.4 431,055 + -1.2 431,182 + Dsui_0426 0.87 -1.6 431,191 - Dsui_0426 0.88 +0.6 431,233 - -0.1 431,254 - +0.3 431,307 - +0.4 431,310 - -1.6
Or see this region's nucleotide sequence