Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0386 and Dsui_0387 are separated by 55 nucleotides Dsui_0387 and Dsui_0388 are separated by 88 nucleotides Dsui_0388 and Dsui_0389 are separated by 12 nucleotides
Dsui_0386: Dsui_0386 - thiol-disulfide isomerase-like thioredoxin, at 385,974 to 386,522
_0386
Dsui_0387: Dsui_0387 - 3-dehydroquinate dehydratase, type II, at 386,578 to 387,078
_0387
Dsui_0388: Dsui_0388 - acetyl-CoA carboxylase, biotin carboxyl carrier protein, at 387,167 to 387,616
_0388
Dsui_0389: Dsui_0389 - acetyl-CoA carboxylase, biotin carboxylase subunit, at 387,629 to 388,996
_0389
Position (kb) 387
388 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 386.170 kb on + strand, within Dsui_0386 at 386.170 kb on + strand, within Dsui_0386 at 386.178 kb on - strand at 386.180 kb on + strand, within Dsui_0386 at 386.214 kb on - strand, within Dsui_0386 at 386.214 kb on - strand, within Dsui_0386 at 386.229 kb on - strand, within Dsui_0386 at 386.280 kb on - strand, within Dsui_0386 at 386.295 kb on + strand, within Dsui_0386 at 386.295 kb on + strand, within Dsui_0386 at 386.303 kb on - strand, within Dsui_0386 at 386.428 kb on + strand, within Dsui_0386 at 386.461 kb on - strand, within Dsui_0386 at 386.570 kb on - strand at 386.575 kb on + strand at 386.613 kb on + strand at 387.086 kb on + strand at 387.086 kb on + strand at 387.086 kb on + strand at 387.134 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 386,170 + Dsui_0386 0.36 -1.8 386,170 + Dsui_0386 0.36 -0.5 386,178 - +0.5 386,180 + Dsui_0386 0.38 -0.4 386,214 - Dsui_0386 0.44 +0.5 386,214 - Dsui_0386 0.44 -1.1 386,229 - Dsui_0386 0.46 -0.5 386,280 - Dsui_0386 0.56 -1.9 386,295 + Dsui_0386 0.58 -0.1 386,295 + Dsui_0386 0.58 -4.5 386,303 - Dsui_0386 0.60 +0.5 386,428 + Dsui_0386 0.83 +0.9 386,461 - Dsui_0386 0.89 +0.5 386,570 - -2.9 386,575 + +4.0 386,613 + +3.6 387,086 + +3.0 387,086 + +3.3 387,086 + +3.7 387,134 + +2.5
Or see this region's nucleotide sequence