Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0270 and Dsui_0271 are separated by 77 nucleotides Dsui_0271 and Dsui_0272 overlap by 4 nucleotides Dsui_0272 and Dsui_0273 are separated by 9 nucleotides Dsui_0273 and Dsui_0274 are separated by 10 nucleotides Dsui_0274 and Dsui_0275 are separated by 216 nucleotides
Dsui_0270: Dsui_0270 - transcriptional regulator, at 271,668 to 272,336
_0270
Dsui_0271: Dsui_0271 - response regulator with CheY-like receiver domain and winged-helix DNA-binding domain, at 272,414 to 273,121
_0271
Dsui_0272: Dsui_0272 - osmosensitive K+ channel histidine kinase, at 273,118 to 275,814
_0272
Dsui_0273: Dsui_0273 - hypothetical protein, at 275,824 to 276,000
_0273
Dsui_0274: Dsui_0274 - hypothetical protein, at 276,011 to 276,313
_0274
Dsui_0275: Dsui_0275 - conserved hypothetical protein, proteobacterial, at 276,530 to 277,366
_0275
Position (kb) 273
274
275
276 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 272.167 kb on + strand, within Dsui_0270 at 272.226 kb on + strand, within Dsui_0270 at 272.478 kb on + strand at 272.502 kb on + strand at 272.751 kb on + strand at 272.830 kb on + strand, within Dsui_0271 at 272.988 kb on - strand, within Dsui_0271 at 273.012 kb on + strand, within Dsui_0271 at 273.012 kb on + strand, within Dsui_0271 at 273.020 kb on - strand, within Dsui_0271 at 273.034 kb on - strand, within Dsui_0271 at 273.034 kb on - strand, within Dsui_0271 at 273.402 kb on + strand, within Dsui_0272 at 273.420 kb on - strand, within Dsui_0272 at 273.459 kb on - strand, within Dsui_0272 at 273.532 kb on + strand at 273.540 kb on - strand at 273.585 kb on - strand, within Dsui_0272 at 273.607 kb on + strand at 273.615 kb on - strand, within Dsui_0272 at 273.729 kb on + strand at 273.799 kb on - strand, within Dsui_0272 at 273.842 kb on + strand, within Dsui_0272 at 273.999 kb on - strand, within Dsui_0272 at 274.014 kb on - strand, within Dsui_0272 at 274.220 kb on - strand at 274.257 kb on - strand at 274.259 kb on - strand, within Dsui_0272 at 274.272 kb on + strand at 274.280 kb on - strand at 274.368 kb on - strand, within Dsui_0272 at 274.484 kb on + strand at 274.535 kb on - strand at 274.536 kb on - strand, within Dsui_0272 at 274.540 kb on + strand at 274.548 kb on - strand, within Dsui_0272 at 274.775 kb on - strand at 274.947 kb on + strand at 275.131 kb on + strand, within Dsui_0272 at 275.134 kb on - strand, within Dsui_0272 at 275.149 kb on - strand, within Dsui_0272 at 275.307 kb on - strand, within Dsui_0272 at 275.317 kb on - strand at 275.381 kb on + strand, within Dsui_0272 at 275.474 kb on - strand at 275.548 kb on + strand at 275.582 kb on + strand at 275.589 kb on - strand at 275.600 kb on - strand at 275.637 kb on - strand at 275.697 kb on - strand at 275.743 kb on + strand at 275.855 kb on - strand, within Dsui_0273 at 275.855 kb on - strand, within Dsui_0273 at 276.002 kb on + strand at 276.030 kb on - strand at 276.095 kb on - strand, within Dsui_0274 at 276.374 kb on + strand at 276.464 kb on + strand at 276.464 kb on + strand at 276.485 kb on + strand at 276.582 kb on - strand at 276.811 kb on + strand, within Dsui_0275
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 272,167 + Dsui_0270 0.75 +0.9 272,226 + Dsui_0270 0.83 +1.0 272,478 + -0.5 272,502 + +0.5 272,751 + -0.5 272,830 + Dsui_0271 0.59 -1.1 272,988 - Dsui_0271 0.81 +0.2 273,012 + Dsui_0271 0.84 -2.1 273,012 + Dsui_0271 0.84 -0.5 273,020 - Dsui_0271 0.86 -1.5 273,034 - Dsui_0271 0.88 -1.1 273,034 - Dsui_0271 0.88 +0.9 273,402 + Dsui_0272 0.11 -0.5 273,420 - Dsui_0272 0.11 -0.5 273,459 - Dsui_0272 0.13 -1.9 273,532 + +0.5 273,540 - +0.5 273,585 - Dsui_0272 0.17 +1.8 273,607 + +0.5 273,615 - Dsui_0272 0.18 +2.3 273,729 + -0.5 273,799 - Dsui_0272 0.25 -1.1 273,842 + Dsui_0272 0.27 +0.5 273,999 - Dsui_0272 0.33 +0.5 274,014 - Dsui_0272 0.33 -2.5 274,220 - +0.5 274,257 - +0.5 274,259 - Dsui_0272 0.42 -2.3 274,272 + +0.5 274,280 - -0.5 274,368 - Dsui_0272 0.46 +0.5 274,484 + -0.5 274,535 - +0.5 274,536 - Dsui_0272 0.53 +0.5 274,540 + +0.5 274,548 - Dsui_0272 0.53 -2.9 274,775 - +0.5 274,947 + +0.5 275,131 + Dsui_0272 0.75 -0.5 275,134 - Dsui_0272 0.75 -0.1 275,149 - Dsui_0272 0.75 -1.1 275,307 - Dsui_0272 0.81 -0.5 275,317 - +0.5 275,381 + Dsui_0272 0.84 -1.1 275,474 - -0.5 275,548 + -0.5 275,582 + +0.5 275,589 - +1.1 275,600 - +2.1 275,637 - -1.8 275,697 - +0.5 275,743 + -1.1 275,855 - Dsui_0273 0.18 +0.5 275,855 - Dsui_0273 0.18 -1.1 276,002 + -1.5 276,030 - -0.5 276,095 - Dsui_0274 0.28 -0.1 276,374 + -0.5 276,464 + -1.1 276,464 + -0.1 276,485 + -1.1 276,582 - +0.5 276,811 + Dsui_0275 0.34 -0.5
Or see this region's nucleotide sequence