Experiment: ALP with Fusidic 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0112 and Dsui_0113 are separated by 21 nucleotides Dsui_0113 and Dsui_0114 are separated by 13 nucleotides
Dsui_0112: Dsui_0112 - serine/threonine protein kinase, at 110,720 to 112,012
_0112
Dsui_0113: Dsui_0113 - hypothetical protein, at 112,034 to 112,297
_0113
Dsui_0114: Dsui_0114 - ammonium transporter, at 112,311 to 113,750
_0114
Position (kb)
112
113 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 111.045 kb on + strand, within Dsui_0112 at 111.048 kb on + strand, within Dsui_0112 at 111.148 kb on - strand, within Dsui_0112 at 111.171 kb on - strand, within Dsui_0112 at 111.262 kb on - strand, within Dsui_0112 at 111.274 kb on + strand, within Dsui_0112 at 111.335 kb on - strand, within Dsui_0112 at 111.440 kb on - strand, within Dsui_0112 at 111.472 kb on + strand, within Dsui_0112 at 111.480 kb on - strand, within Dsui_0112 at 111.504 kb on - strand, within Dsui_0112 at 111.507 kb on + strand, within Dsui_0112 at 111.515 kb on - strand, within Dsui_0112 at 111.532 kb on + strand, within Dsui_0112 at 111.540 kb on - strand at 111.654 kb on - strand at 111.656 kb on + strand, within Dsui_0112 at 111.664 kb on - strand, within Dsui_0112 at 111.738 kb on - strand, within Dsui_0112 at 111.753 kb on + strand, within Dsui_0112 at 111.866 kb on + strand, within Dsui_0112 at 111.867 kb on + strand, within Dsui_0112 at 111.877 kb on + strand, within Dsui_0112 at 111.885 kb on - strand at 111.892 kb on + strand at 111.905 kb on + strand at 111.910 kb on + strand at 111.988 kb on - strand at 112.040 kb on - strand at 112.052 kb on - strand at 112.059 kb on + strand at 112.060 kb on - strand at 112.228 kb on + strand, within Dsui_0113 at 112.236 kb on - strand, within Dsui_0113 at 112.268 kb on - strand at 112.299 kb on + strand at 112.300 kb on - strand at 112.300 kb on - strand at 112.300 kb on - strand at 112.385 kb on + strand at 112.389 kb on - strand at 112.431 kb on + strand at 112.434 kb on - strand at 112.562 kb on + strand, within Dsui_0114 at 112.562 kb on + strand, within Dsui_0114 at 112.570 kb on - strand, within Dsui_0114 at 112.601 kb on - strand at 112.613 kb on + strand, within Dsui_0114 at 112.620 kb on + strand, within Dsui_0114 at 112.623 kb on - strand, within Dsui_0114 at 112.740 kb on + strand at 112.748 kb on - strand, within Dsui_0114 at 112.763 kb on - strand, within Dsui_0114 at 112.886 kb on - strand at 112.913 kb on - strand, within Dsui_0114 at 112.918 kb on - strand, within Dsui_0114 at 112.928 kb on - strand, within Dsui_0114 at 112.935 kb on + strand, within Dsui_0114 at 112.941 kb on + strand at 112.949 kb on - strand, within Dsui_0114 at 112.966 kb on + strand at 112.990 kb on + strand, within Dsui_0114 at 112.990 kb on + strand at 112.990 kb on + strand, within Dsui_0114 at 113.030 kb on + strand, within Dsui_0114 at 113.115 kb on + strand at 113.146 kb on + strand at 113.173 kb on - strand, within Dsui_0114 at 113.187 kb on + strand, within Dsui_0114 at 113.255 kb on + strand at 113.287 kb on - strand, within Dsui_0114
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Fusidic 0.01 mg/ml remove 111,045 + Dsui_0112 0.25 -0.3 111,048 + Dsui_0112 0.25 -1.4 111,148 - Dsui_0112 0.33 -1.3 111,171 - Dsui_0112 0.35 +0.2 111,262 - Dsui_0112 0.42 -1.6 111,274 + Dsui_0112 0.43 -0.8 111,335 - Dsui_0112 0.48 +0.7 111,440 - Dsui_0112 0.56 +0.7 111,472 + Dsui_0112 0.58 +0.7 111,480 - Dsui_0112 0.59 -2.1 111,504 - Dsui_0112 0.61 -2.8 111,507 + Dsui_0112 0.61 +1.1 111,515 - Dsui_0112 0.61 -0.8 111,532 + Dsui_0112 0.63 -0.3 111,540 - +0.7 111,654 - +0.7 111,656 + Dsui_0112 0.72 +0.3 111,664 - Dsui_0112 0.73 +0.7 111,738 - Dsui_0112 0.79 -1.3 111,753 + Dsui_0112 0.80 -1.3 111,866 + Dsui_0112 0.89 -0.8 111,867 + Dsui_0112 0.89 -1.6 111,877 + Dsui_0112 0.89 +0.7 111,885 - +0.7 111,892 + -1.3 111,905 + -1.3 111,910 + +1.0 111,988 - +0.7 112,040 - -1.6 112,052 - -1.3 112,059 + -0.3 112,060 - -0.3 112,228 + Dsui_0113 0.73 +0.7 112,236 - Dsui_0113 0.77 -0.3 112,268 - +0.7 112,299 + +0.7 112,300 - +0.7 112,300 - -2.4 112,300 - +0.7 112,385 + -0.3 112,389 - -2.1 112,431 + +0.7 112,434 - +0.7 112,562 + Dsui_0114 0.17 +0.7 112,562 + Dsui_0114 0.17 -2.1 112,570 - Dsui_0114 0.18 -0.3 112,601 - +0.7 112,613 + Dsui_0114 0.21 +0.7 112,620 + Dsui_0114 0.21 -0.3 112,623 - Dsui_0114 0.22 -0.3 112,740 + -0.8 112,748 - Dsui_0114 0.30 -0.8 112,763 - Dsui_0114 0.31 -0.8 112,886 - +0.7 112,913 - Dsui_0114 0.42 -0.3 112,918 - Dsui_0114 0.42 -0.3 112,928 - Dsui_0114 0.43 +0.7 112,935 + Dsui_0114 0.43 -0.3 112,941 + +0.7 112,949 - Dsui_0114 0.44 -1.3 112,966 + +0.7 112,990 + Dsui_0114 0.47 +0.7 112,990 + +0.7 112,990 + Dsui_0114 0.47 -1.3 113,030 + Dsui_0114 0.50 -0.3 113,115 + +2.3 113,146 + +0.7 113,173 - Dsui_0114 0.60 -1.3 113,187 + Dsui_0114 0.61 +0.0 113,255 + +0.7 113,287 - Dsui_0114 0.68 -0.3
Or see this region's nucleotide sequence