Experiment: Formate/Acetate-Thiosulfate (60/30-15 mM) + 0.1% Yeast Extract
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cas3 and DVUA0130 are separated by 13 nucleotides DVUA0130 and DVUA0131 overlap by 4 nucleotides
DVUA0129: cas3 - CRISPR-associated helicase Cas3 domain protein (TIGR), at 168,254 to 170,362
cas3
DVUA0130: DVUA0130 - CRISPR-associated protein, CT1134 family (TIGR), at 170,376 to 171,059
DVUA0130
DVUA0131: DVUA0131 - CRISPR-associated protein, CT1133 family (TIGR), at 171,056 to 172,894
DVUA0131
Position (kb) 170
171
172 Strain fitness (log2 ratio) -2
-1
0
1
2 at 169.392 kb on + strand, within cas3 at 169.392 kb on - strand, within cas3 at 169.405 kb on - strand, within cas3 at 169.438 kb on + strand, within cas3 at 169.438 kb on + strand, within cas3 at 169.446 kb on - strand, within cas3 at 169.480 kb on - strand, within cas3 at 169.486 kb on + strand, within cas3 at 169.532 kb on - strand, within cas3 at 169.661 kb on - strand, within cas3 at 169.747 kb on - strand, within cas3 at 169.795 kb on - strand, within cas3 at 169.820 kb on - strand, within cas3 at 169.854 kb on - strand, within cas3 at 169.879 kb on - strand, within cas3 at 169.879 kb on - strand, within cas3 at 169.879 kb on - strand, within cas3 at 169.937 kb on + strand, within cas3 at 169.966 kb on + strand, within cas3 at 169.966 kb on + strand, within cas3 at 169.966 kb on + strand, within cas3 at 169.974 kb on - strand, within cas3 at 170.115 kb on + strand, within cas3 at 170.123 kb on + strand, within cas3 at 170.166 kb on + strand at 170.166 kb on + strand at 170.166 kb on + strand at 170.174 kb on - strand at 170.174 kb on - strand at 170.179 kb on - strand at 170.181 kb on + strand at 170.268 kb on + strand at 170.304 kb on + strand at 170.333 kb on - strand at 170.404 kb on + strand at 170.425 kb on - strand at 170.489 kb on - strand, within DVUA0130 at 170.509 kb on + strand, within DVUA0130 at 170.510 kb on + strand, within DVUA0130 at 170.517 kb on - strand, within DVUA0130 at 170.560 kb on + strand, within DVUA0130 at 170.565 kb on - strand, within DVUA0130 at 170.702 kb on - strand, within DVUA0130 at 170.763 kb on + strand, within DVUA0130 at 170.796 kb on + strand, within DVUA0130 at 170.959 kb on - strand, within DVUA0130 at 171.135 kb on + strand at 171.153 kb on - strand at 171.355 kb on + strand, within DVUA0131 at 171.363 kb on - strand, within DVUA0131 at 171.379 kb on + strand, within DVUA0131 at 171.387 kb on - strand, within DVUA0131 at 171.404 kb on + strand, within DVUA0131 at 171.532 kb on + strand, within DVUA0131 at 171.585 kb on + strand, within DVUA0131 at 171.607 kb on - strand, within DVUA0131 at 171.607 kb on - strand, within DVUA0131 at 171.612 kb on + strand, within DVUA0131 at 171.655 kb on + strand, within DVUA0131 at 171.663 kb on - strand, within DVUA0131 at 171.712 kb on - strand, within DVUA0131 at 171.789 kb on + strand, within DVUA0131 at 171.800 kb on + strand, within DVUA0131 at 171.800 kb on + strand, within DVUA0131 at 171.838 kb on + strand, within DVUA0131 at 171.865 kb on + strand, within DVUA0131 at 171.873 kb on - strand, within DVUA0131 at 171.888 kb on - strand, within DVUA0131 at 171.893 kb on + strand, within DVUA0131 at 171.930 kb on - strand, within DVUA0131 at 171.991 kb on + strand, within DVUA0131
Per-strain Table
Position Strand Gene LocusTag Fraction Formate/Acetate-Thiosulfate (60/30-15 mM) + 0.1% Yeast Extract remove 169,392 + cas3 DVUA0129 0.54 -0.9 169,392 - cas3 DVUA0129 0.54 +1.5 169,405 - cas3 DVUA0129 0.55 -0.7 169,438 + cas3 DVUA0129 0.56 -0.2 169,438 + cas3 DVUA0129 0.56 +2.7 169,446 - cas3 DVUA0129 0.57 -0.2 169,480 - cas3 DVUA0129 0.58 +0.5 169,486 + cas3 DVUA0129 0.58 -0.4 169,532 - cas3 DVUA0129 0.61 -1.6 169,661 - cas3 DVUA0129 0.67 -1.2 169,747 - cas3 DVUA0129 0.71 +0.3 169,795 - cas3 DVUA0129 0.73 -0.6 169,820 - cas3 DVUA0129 0.74 +0.1 169,854 - cas3 DVUA0129 0.76 -0.3 169,879 - cas3 DVUA0129 0.77 -0.4 169,879 - cas3 DVUA0129 0.77 -1.6 169,879 - cas3 DVUA0129 0.77 -1.1 169,937 + cas3 DVUA0129 0.80 -0.1 169,966 + cas3 DVUA0129 0.81 -0.3 169,966 + cas3 DVUA0129 0.81 +0.2 169,966 + cas3 DVUA0129 0.81 -0.4 169,974 - cas3 DVUA0129 0.82 -0.2 170,115 + cas3 DVUA0129 0.88 -0.3 170,123 + cas3 DVUA0129 0.89 +0.4 170,166 + -0.5 170,166 + -0.8 170,166 + -0.2 170,174 - -0.3 170,174 - -0.8 170,179 - -0.1 170,181 + -0.5 170,268 + -0.4 170,304 + -0.3 170,333 - +0.4 170,404 + -0.0 170,425 - -0.3 170,489 - DVUA0130 0.17 -0.5 170,509 + DVUA0130 0.19 -0.6 170,510 + DVUA0130 0.20 -0.7 170,517 - DVUA0130 0.21 -0.1 170,560 + DVUA0130 0.27 -1.1 170,565 - DVUA0130 0.28 -0.1 170,702 - DVUA0130 0.48 -0.9 170,763 + DVUA0130 0.57 -0.1 170,796 + DVUA0130 0.61 -0.3 170,959 - DVUA0130 0.85 -0.9 171,135 + -0.1 171,153 - -0.8 171,355 + DVUA0131 0.16 -1.3 171,363 - DVUA0131 0.17 -0.2 171,379 + DVUA0131 0.18 +0.5 171,387 - DVUA0131 0.18 -0.8 171,404 + DVUA0131 0.19 -1.8 171,532 + DVUA0131 0.26 +0.2 171,585 + DVUA0131 0.29 -0.1 171,607 - DVUA0131 0.30 -0.3 171,607 - DVUA0131 0.30 -0.5 171,612 + DVUA0131 0.30 -0.8 171,655 + DVUA0131 0.33 +1.5 171,663 - DVUA0131 0.33 -0.4 171,712 - DVUA0131 0.36 +0.1 171,789 + DVUA0131 0.40 +0.5 171,800 + DVUA0131 0.40 -0.7 171,800 + DVUA0131 0.40 -1.3 171,838 + DVUA0131 0.43 -1.2 171,865 + DVUA0131 0.44 -0.7 171,873 - DVUA0131 0.44 -0.3 171,888 - DVUA0131 0.45 -2.2 171,893 + DVUA0131 0.46 -0.3 171,930 - DVUA0131 0.48 +1.3 171,991 + DVUA0131 0.51 -0.7
Or see this region's nucleotide sequence