Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1223

Experiment: Aluminum chloride 0.00005 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1221 and Pf6N2E2_1222 are separated by 148 nucleotidesPf6N2E2_1222 and Pf6N2E2_1223 are separated by 153 nucleotidesPf6N2E2_1223 and Pf6N2E2_1224 are separated by 114 nucleotides Pf6N2E2_1221 - sodium/hydrogen exchanger family protein, at 1,427,660 to 1,428,997 _1221 Pf6N2E2_1222 - FIG00954310: hypothetical protein, at 1,429,146 to 1,429,682 _1222 Pf6N2E2_1223 - Amino acid transporters, at 1,429,836 to 1,430,066 _1223 Pf6N2E2_1224 - Two-component hybrid sensor and regulator, at 1,430,181 to 1,432,109 _1224 Position (kb) 1429 1430 1431Strain fitness (log2 ratio) -2 -1 0 1at 1428.842 kb on + strand, within Pf6N2E2_1221at 1428.842 kb on + strand, within Pf6N2E2_1221at 1428.971 kb on + strandat 1428.971 kb on + strandat 1428.971 kb on + strandat 1428.972 kb on - strandat 1428.999 kb on + strandat 1429.173 kb on - strandat 1429.174 kb on + strandat 1429.175 kb on - strandat 1429.175 kb on - strandat 1429.175 kb on - strandat 1429.175 kb on - strandat 1429.183 kb on + strandat 1429.183 kb on + strandat 1429.226 kb on + strand, within Pf6N2E2_1222at 1429.226 kb on + strand, within Pf6N2E2_1222at 1429.226 kb on + strand, within Pf6N2E2_1222at 1429.226 kb on + strand, within Pf6N2E2_1222at 1429.226 kb on + strand, within Pf6N2E2_1222at 1429.227 kb on - strand, within Pf6N2E2_1222at 1429.227 kb on - strand, within Pf6N2E2_1222at 1429.227 kb on - strand, within Pf6N2E2_1222at 1429.304 kb on - strand, within Pf6N2E2_1222at 1429.335 kb on + strand, within Pf6N2E2_1222at 1429.534 kb on + strand, within Pf6N2E2_1222at 1429.534 kb on + strand, within Pf6N2E2_1222at 1429.534 kb on + strand, within Pf6N2E2_1222at 1429.534 kb on + strand, within Pf6N2E2_1222at 1429.534 kb on + strand, within Pf6N2E2_1222at 1429.535 kb on - strand, within Pf6N2E2_1222at 1429.535 kb on - strand, within Pf6N2E2_1222at 1429.535 kb on - strand, within Pf6N2E2_1222at 1429.672 kb on - strandat 1429.720 kb on - strandat 1429.809 kb on - strandat 1429.809 kb on - strandat 1429.809 kb on - strandat 1429.843 kb on - strandat 1429.946 kb on + strand, within Pf6N2E2_1223at 1429.958 kb on + strand, within Pf6N2E2_1223at 1429.958 kb on + strand, within Pf6N2E2_1223at 1429.985 kb on + strand, within Pf6N2E2_1223at 1430.183 kb on - strandat 1430.183 kb on - strandat 1430.282 kb on - strandat 1430.407 kb on + strand, within Pf6N2E2_1224at 1430.613 kb on - strand, within Pf6N2E2_1224at 1430.798 kb on - strand, within Pf6N2E2_1224at 1430.989 kb on - strand, within Pf6N2E2_1224at 1431.027 kb on - strand, within Pf6N2E2_1224at 1431.061 kb on + strand, within Pf6N2E2_1224

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Per-strain Table

Position Strand Gene LocusTag Fraction Aluminum chloride 0.00005 M
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1,428,842 + Pf6N2E2_1221 0.88 +0.1
1,428,842 + Pf6N2E2_1221 0.88 -1.2
1,428,971 + +0.5
1,428,971 + +0.0
1,428,971 + +1.0
1,428,972 - -1.4
1,428,999 + -0.4
1,429,173 - +0.2
1,429,174 + -0.4
1,429,175 - +0.9
1,429,175 - -0.6
1,429,175 - -0.9
1,429,175 - +1.2
1,429,183 + +0.8
1,429,183 + -0.8
1,429,226 + Pf6N2E2_1222 0.15 +0.3
1,429,226 + Pf6N2E2_1222 0.15 +0.0
1,429,226 + Pf6N2E2_1222 0.15 -1.8
1,429,226 + Pf6N2E2_1222 0.15 +0.9
1,429,226 + Pf6N2E2_1222 0.15 +0.9
1,429,227 - Pf6N2E2_1222 0.15 +0.0
1,429,227 - Pf6N2E2_1222 0.15 +0.5
1,429,227 - Pf6N2E2_1222 0.15 -0.9
1,429,304 - Pf6N2E2_1222 0.29 +0.3
1,429,335 + Pf6N2E2_1222 0.35 -0.8
1,429,534 + Pf6N2E2_1222 0.72 +0.6
1,429,534 + Pf6N2E2_1222 0.72 +0.2
1,429,534 + Pf6N2E2_1222 0.72 -1.8
1,429,534 + Pf6N2E2_1222 0.72 +1.0
1,429,534 + Pf6N2E2_1222 0.72 +0.2
1,429,535 - Pf6N2E2_1222 0.72 -0.6
1,429,535 - Pf6N2E2_1222 0.72 -0.0
1,429,535 - Pf6N2E2_1222 0.72 -0.4
1,429,672 - -0.6
1,429,720 - -0.8
1,429,809 - +0.3
1,429,809 - +0.6
1,429,809 - -0.4
1,429,843 - +1.0
1,429,946 + Pf6N2E2_1223 0.48 -1.2
1,429,958 + Pf6N2E2_1223 0.53 -1.0
1,429,958 + Pf6N2E2_1223 0.53 +0.6
1,429,985 + Pf6N2E2_1223 0.65 -0.6
1,430,183 - +0.1
1,430,183 - -1.6
1,430,282 - +0.3
1,430,407 + Pf6N2E2_1224 0.12 +0.2
1,430,613 - Pf6N2E2_1224 0.22 +0.0
1,430,798 - Pf6N2E2_1224 0.32 +0.9
1,430,989 - Pf6N2E2_1224 0.42 +0.8
1,431,027 - Pf6N2E2_1224 0.44 -0.6
1,431,061 + Pf6N2E2_1224 0.46 -0.4

Or see this region's nucleotide sequence